Date published: 2025-9-9
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SERCA2 Antibody (F-1) - Western Blotting - Image 352702
SERCA2 抗体 (F-1): sc-376235. A-431 (A), K-562 (B), THP-1 (C), A-673 (D), MCF7 (E) 和 NIH/3T3 (F) 全细胞裂解液中 SERCA2 表达的 Western 印迹分析.
SERCA2 抗体 (F-1): sc-376235. A-431 (A), K-562 (B), THP-1 (C), A-673 (D), MCF7 (E) 和 NIH/3T3 (F) 全细胞裂解液中 SERCA2 表达的 Western 印迹分析.

SERCA2 抗体 (F-1): sc-376235

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寫評論提問

说明书
  • SERCA2抗体(F-1)是小鼠单克隆IgG1 κ, 在53篇文献中引用，规格为200 µg/ml
  • 特异性抗原位于human物种的SERCA2的N-terminus的氨基酸2-29之间
  • 抗-SERCA2 抗体 (F-1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 SERCA2; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗SERCA2抗体（F-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取SERCA2 (F-1): sc-376235的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP是SERCA2 Antibody (F-1) 适用于 WB 和 IHC(P) 应用。 的首选辅助检测试剂。该试剂现与SERCA2 Antibody (F-1) 搭配使用（请参阅下面的订购信息）。

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描述
基因信息
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研究信息

関連項目

额外SERCA抗体，包括SERCA1, SERCA2和SERCA3

SERCA2抗体（F-1）是一种IgG1 κ小鼠单克隆SERCA2抗体，可通过WB、IP、IF、IHC(P)和ELISA检测鼠、大鼠和人源的SERCA2。SERCA2抗体（F-1）既可以是非偶联的抗SERCA2抗体形式，也可以是多种偶联形式的抗SERCA2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。ATP依赖的钙泵部分负责维持细胞质中游离钙的低浓度。位于细胞内细胞器中的ATP泵由一系列结构相关的酶编码，称为肌质网或内质网钙（SERCA）ATP酶。横纹肌的肌质网是一个特殊的细胞内膜系统，在肌肉的收缩和松弛中起着关键作用。SERCAs介导Ca2+进入细胞内储存。SERCA介导的Ca2+摄取诱导并维持肌肉松弛。SERCA1基因仅在II型（快）骨骼肌中表达。SERCA2基因受到组织依赖性处理的影响，负责产生在I型（慢）骨骼、心脏和平滑肌中表达的SERCA2a肌肉特异性形式，以及在所有细胞类型中表达的SERCA2b异型。SERCA3基因的特征不明显，存在于非肌肉细胞中。SERCA2在调节心脏收缩功能中发挥重要作用。SERCA3是一种在多种细胞类型中表达的异型，包括血小板、淋巴细胞和肥大细胞。SERCA1、SERCA2和SERCA3都经历选择性剪接。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SERCA2 抗体 (F-1) 参考文献:

  1. SERCA2调节非CF和CF气道上皮细胞对臭氧的反应。  |  Ahmad, S., et al. 2011. PLoS One. 6: e27451. PMID: 22096575
  2. 达里尔病小鼠模型中的SERCA2单基因不足会导致选择性心力衰竭。  |  Prasad, V., et al. 2015. Biomed Res Int. 2015: 251598. PMID: 26064889
  3. HIF-1对miR-29c的调节会损害SERCA2的表达和心脏收缩力。  |  Williams, AL., et al. 2019. Am J Physiol Heart Circ Physiol. 316: H554-H565. PMID: 30575439
  4. LncEDCH1通过与SERCA2相互作用，改善线粒体功能，减少肌肉萎缩。  |  Cai, B., et al. 2022. Mol Ther Nucleic Acids. 27: 319-334. PMID: 35024244
  5. 脂肪细胞特异性Ca2+泵SERCA2的缺失会损害全身代谢功能，并揭示白色和棕色脂肪组织的不同代谢灵活性。  |  Bauzá-Thorbrügge, M., et al. 2022. Mol Metab. 63: 101535. PMID: 35760318
  6. 远程缺血后适应通过SERCA2/内质网应激介导的细胞凋亡减轻脑缺血损伤。  |  Chen, W., et al. 2024. Microsc Res Tech. 87: 424-433. PMID: 37897375
  7. 血小板中第三种肌浆/内质网 Ca2+ ATP 酶（SERCA3）两种同工酶的表达。PL/IM430单克隆抗体可能识别SERCA3b异构体。  |  Bobe, R., et al. 1998. FEBS Lett. 423: 259-64. PMID: 9512369
  8. 钙 ATP 酶与呼吸肌功能  |  Aubier, M. and Viires, N. 1998. Eur Respir J. 11: 758-66. PMID: 9596133
  9. 小鼠 SERCA 3 基因 3' 端的特征和 mRNA 剪接变体的组织分布。  |  Ozog, A., et al. 1998. FEBS Lett. 427: 349-52. PMID: 9637255
  10. 表达快肌骨骼肌肌浆网 Ca2+-ATP 酶的转基因心脏心肌收缩力增强，Ca2+ 转运功能增强。  |  Loukianov, E., et al. 1998. Circ Res. 83: 889-97. PMID: 9797337
  11. 主动脉瓣狭窄诱导的大鼠心脏肥大中 Ca2+ 泵和 Ca2+ 释放通道的细胞分布。  |  Anger, M., et al. 1998. Circulation. 98: 2477-86. PMID: 9832495
  12. 替代剪接的人类 SERCA3 转录本的功能特征。  |  Poch, E., et al. 1998. Am J Physiol. 275: C1449-58. PMID: 9843705

订购信息

产品名称产品编号规格价格数量收藏夹

SERCA2 抗体 (F-1)

sc-376235
200 µg/ml
$316.00

SERCA2 (F-1): m-IgG Fc BP-HRP 套装

sc-551136
200 µg Ab; 10 µg BP
$354.00

SERCA2 抗体 (F-1) AC

sc-376235 AC
500 µg/ml, 25% agarose
$416.00

SERCA2 抗体 (F-1) HRP

sc-376235 HRP
200 µg/ml
$316.00

SERCA2 抗体 (F-1) FITC

sc-376235 FITC
200 µg/ml
$330.00

SERCA2 抗体 (F-1) PE

sc-376235 PE
200 µg/ml
$343.00

SERCA2 抗体 (F-1) Alexa Fluor® 488

sc-376235 AF488
200 µg/ml
$357.00

SERCA2 抗体 (F-1) Alexa Fluor® 546

sc-376235 AF546
200 µg/ml
$357.00

SERCA2 抗体 (F-1) Alexa Fluor® 594

sc-376235 AF594
200 µg/ml
$357.00

SERCA2 抗体 (F-1) Alexa Fluor® 647

sc-376235 AF647
200 µg/ml
$357.00

SERCA2 抗体 (F-1) Alexa Fluor® 680

sc-376235 AF680
200 µg/ml
$357.00

SERCA2 抗体 (F-1) Alexa Fluor® 790

sc-376235 AF790
200 µg/ml
$357.00

SERCA2 (F-1) 中和勝肽

sc-376235 P
100 µg/0.5 ml
$68.00

这个多大规格啊，价格和货期可以发一下吗

Asked by: Anonymous
感谢您的提问。SERCA2 抗体 (F-1): sc-376235的规格为200 ug/ml, 1ml 的体积。价格和货期，请咨询您当地的经销商，或者联系我们，电话：021-60936350，或者邮箱：asia@scbio.cn。 Thank you for your question. SERCA2 antibody (F-1): sc-376235 is provided at 200 ug/ml, the volumn is 1ml. As to the quotation and lead time, please contact your local distributors or SCBT Shanghai office via phone #: 021-60936350, or email address: asia@scbio.cn.
Answered by: Technical Service SH
Date published: 2023-11-13

What blocking solution (milk og BSA) is reccomended for this antibody? And what dilution would you reccomend for western blotting? Incubation over night?

Asked by: Øyvind
Thank you for your question. We recommend using 5% milk in TBST (available for purchase as TBS Blotto A: sc-2333) for both blocking and antibody incubations. We recommend a starting dilution of 1:200 for Western blotting with a 1 hour incubation at room temperature, or overnight at 4C.
Answered by: Technical Support
Date published: 2018-11-30

Can this SERCA2 antibody be used for IP? What is the recommended dilution? Thanks.

Asked by: ZTan
Thank you for your question. This SERCA2 antibody is fine for Immunoprecipitation, and you can find the recommended protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody per 1 ml of cell lysate. If you have any further questions, please reach out to Technical support at scbt@scbt.com, or via phone at 800-457-3801, ext 2 or via chat directly on our website, www.scbt.com.
Answered by: Tech Service
Date published: 2022-11-15

Hello, Would it be possible to obtain FREE 10 µg sample of SERCA2 (F-1) HRP: sc-376235 HRP. I've never used primary antibody already conjugated with HRP but I'd like to give it a try.

Asked by: Tomasz Bednarski
For a limited time, we are offering a free 10 µg sample of the unconjugated antibody. We do not provide samples of the conjugated antibody, but it is available for purchase and covered 100% by our product warranty. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-11-17

What is the total volume provided of SERCA2 (F-1):sc-376235 monoclonal antibody?

Asked by: jenni
Thank you for your question. This antibody is provided as 200 µg in 1 ml total volume of PBS with < 0.1% sodium azide and 0.1% gelatin.
Answered by: Technical Support 7
Date published: 2017-01-13

Can SERCA2 (F-1): sc-376235 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenni
Thank you for your question. Yes, SERCA2 (F-1): sc-376235 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 7
Date published: 2017-01-13
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Rated 5 out of 5 by from WB 1:1000，it works well抗体1:1000稀释用于WB， 曝光条带清晰，回收后使用，依旧能获得满意的条带，表现比abcam同靶点好。
Date published: 2024-04-17
Rated 5 out of 5 by from trés bonne détection en immunofluorescenceBonne détection sur des marquages de cellules MCF7
Date published: 2018-02-19
Rated 4 out of 5 by from Works great for western blots!I've used this at 1:500 on mouse pancreatic islets with great results, with very little background.
Date published: 2018-02-13
Rated 5 out of 5 by from Works very well in mouse muscleWorks very well for western blotting on mouse skeletal muscle samples - 1:1000 3% BSA.
Date published: 2018-01-11
Rated 3 out of 5 by from Reliable antibody working well on mouse samplesThe antibody produces several bands ranging from 90 to 130 kDa as indicated on the data sheet.
Date published: 2017-11-27
Rated 5 out of 5 by from Great Antibody - SERCA2 (F-1)I am really happy that we got clear band with SERCA2 (F-1). As our samples are porcine origin, it is hard to find the right one. I will recommend this antibody to other users.
Date published: 2017-10-18
Rated 4 out of 5 by from Strong signal of SERCA2 in WBUsed at 1:100 000 dilution. samples were not reduced or heated. 100 ug of rat LV/heart lysates loaded onto 8% gel. Transfer onto 0.2 nm nitrocellulose membrane and probed with 1:10000 LiCor IRDye 800CW anti-mouse seconday antibody.
Date published: 2017-09-25
Rated 5 out of 5 by from Work well in WBI've got a 10ug sample of this antibody. It works well in WB at 1:200, MDA-MB-231 cells, T47D cells.
Date published: 2017-09-19
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SERCA2 抗体 (F-1) is rated 4.7 out of 5 by 20.
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