Date published: 2025-9-7

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SEC22B 抗体 (29-F7): sc-101267

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说明书
  • SEC22B抗体(29-F7)是小鼠单克隆IgG2a λ SEC22B抗体, 在22篇文献中引用,规格为100 µg/ml
  • 针对部分重组蛋白,其氨基酸序列在SEC22B的human起源的1-110范围内
  • 推荐用于 mouse, rat 和 human 来源的SEC22B WB, IP, IF 和 ELISA检测
  • 关于如何获取SEC22B (29-F7): sc-101267免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRP是SEC22B Antibody (29-F7) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与SEC22B Antibody (29-F7) 打包提供(请参阅下面的订购信息)。

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関連項目

SEC22B抗体(29-F7)是一种IgG2a λ小鼠单克隆SEC22B抗体(也被称为SEC22B抗体),可通过WB、IP、IF和ELISA检测到小鼠、大鼠和人类来源的SEC22B蛋白。SEC22B抗体(29-F7)可作为非偶联抗SEC22B抗体使用。酿酒酵母蛋白Sec22p作为运输囊泡的v-SNARE起作用,并在高尔基体和内质网(ER)之间的逆行和前向囊泡运输中发挥作用。Sec22p有三个哺乳动物同源物,即SEC22A、SEC22B和SEC22C。SEC22B(SEC22囊泡转运蛋白同源物B,酿酒酵母),也被称为SEC22L1(SEC22囊泡转运蛋白样1)或ERS-24,是一种属于突触囊泡蛋白家族的单次跨膜IV型膜蛋白。SEC22B包含一个v-SNARE卷曲螺旋同源结构域和一个longin结构域。SEC22B定位于内质网-高尔基体中间区室,并存在于内质网衍生囊泡上,作为v-SNARE起作用,是内质网-高尔基体转运所必需的。位于高尔基前中间区室的同源t-SNARE由Syntaxin 5、Bet1和GS27组成。此外,SEC22B可与Syntaxin 18、Nip1和USE1(p31)一起存在于SNARE复合物中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

SEC22B 抗体 (29-F7) 参考文献:

  1. 军团菌颠覆了 Rab1 和 Sec22b 的功能,创建了一个复制细胞器。  |  Kagan, JC., et al. 2004. J Exp Med. 199: 1201-11. PMID: 15117975
  2. J774 巨噬细胞不同程度地利用内质网膜进行吞噬。  |  Becker, T., et al. 2005. Proc Natl Acad Sci U S A. 102: 4022-6. PMID: 15753287
  3. hsc70伴侣调节ER/高尔基体SNARE复合体形成的证据  |  Joglekar, AP. and Hay, JC. 2005. Eur J Cell Biol. 84: 529-42. PMID: 16003907
  4. ER出口位点的免疫电子断层扫描显示了游离的COPII包被运输载体的存在。  |  Zeuschner, D., et al. 2006. Nat Cell Biol. 8: 377-83. PMID: 16531996
  5. 内质网(ER)定域 SNARE 蛋白 syntaxin 18 参与 ER 介导的吞噬作用。  |  Hatsuzawa, K., et al. 2006. Mol Biol Cell. 17: 3964-77. PMID: 16790498
  6. 鉴定酵母 R-SNARE Nyv1p 是一种新型含长蛋白结构域的蛋白质。  |  Wen, W., et al. 2006. Mol Biol Cell. 17: 4282-99. PMID: 16855025
  7. 依赖于 Sec22b 的内质网 Q-SNARE 蛋白组装。  |  Aoki, T., et al. 2008. Biochem J. 410: 93-100. PMID: 17979832
  8. 鉴定VLDL转运囊泡与肝顺-高尔基体融合所需的SNARE复合物  |  Siddiqi, S., et al. 2010. Biochem J. 429: 391-401. PMID: 20450495
  9. 肝癌患者通过SEC22B+囊泡途径参与非常规蛋白分泌  |  Yamada, K., et al. 2022. Proc Natl Acad Sci U S A. 119: e2202730119. PMID: 36044553
  10. Sec22b是浆细胞维持的关键和非冗余调节因子。  |  Bonaud, A., et al. 2023. Proc Natl Acad Sci U S A. 120: e2213056120. PMID: 36595686
  11. 浆细胞的积累需要一个 Sec(22b)。  |  Mulder, J., et al. 2023. Immunol Cell Biol. 101: 285-288. PMID: 36789450
  12. Sec22b/ERS-24 与前高尔基体中间区室的形态和功能缔合作用。  |  Zhang, T., et al. 1999. Mol Biol Cell. 10: 435-53. PMID: 9950687

订购信息

产品名称产品编号规格价格数量收藏夹

SEC22B 抗体 (29-F7)

sc-101267
100 µg/ml
$333.00

SEC22B (29-F7): m-IgG Fc BP-HRP 套装

sc-539746
100 µg Ab; 10 µg BP
$354.00

SEC22B (29-F7): m-IgG2a BP-HRP 套装

sc-546781
100 µg Ab; 10 µg BP
$354.00

Can this antibody be used to detect SEC22B in canine samples?

Asked by: AbPolly
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 9
Date published: 2017-01-12

Can sc-516102 be used with this antibody?

Asked by: AbPolly
Thank you for your question. sc-516102 is specific to the kappa light chain, and this antibody is lambda light chain. sc-516132 would be the appropriate HRP secondary for this antibody. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 9
Date published: 2017-01-12
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Rated 5 out of 5 by from Single band in WBWestern blot に使用しました。Human、mouseどちらの細胞でも綺麗に検出することができました。
Date published: 2020-08-24
Rated 1 out of 5 by from No IF signals in mice/rat mandible ameloblastsThis antibody doesn't show bindings on PFA fixed and paraffin embedded sections from mice/rat mandible ameloblasts.
Date published: 2017-07-06
Rated 3 out of 5 by from Not usable on J774A.1 cellsProduces dim diffuse staining with green blobs that I can't use for determining co-localization with intracellular pathogens in J774A.1 cells. On the other hand, it was a free sample, so no harm done.
Date published: 2017-05-11
Rated 5 out of 5 by from Strong westernVery nice western blot data; strong signal with low background
Date published: 2017-01-31
Rated 5 out of 5 by from Great detection.This antibody is reactive with mouse, rat, and human. It will provide strong WB bands and specific signal in your IF. This antibody has been published with several times.
Date published: 2017-01-30
Rated 4 out of 5 by from Produced good immunofluorescence nuclearProduced good immunofluorescence nuclear and cytoplasmic staining in paraformaldehyde-fixed HeLa cells. -SCBT QC
Date published: 2015-02-25
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SEC22B 抗体 (29-F7) is rated 3.9 out of 5 by 7.
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