Date published: 2025-9-8
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Rock-2 Antibody (D-11) - Western Blotting - Image 305692
Rock-2 抗体 (D-11): sc-398519. HeLa (A), A-673 (B), Y79 (C), MCF7 (D) 和 Jurkat (E) 全细胞裂解液中 Rock-2 表达的 Western 印迹分析.
Rock-2 抗体 (D-11): sc-398519. HeLa (A), A-673 (B), Y79 (C), MCF7 (D) 和 Jurkat (E) 全细胞裂解液中 Rock-2 表达的 Western 印迹分析.

Rock-2 抗体 (D-11): sc-398519

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寫評論提問

说明书
  • Rock-2 抗体 D-11 是小鼠单克隆 IgG2a κ，Rock-2抗体, 在42篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列775-860位于human物种的Rock-2的内部区域内
  • Rock-2 抗体 (D-11) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Rock-2
  • 抗Rock-2抗体（D-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Rock-2 (D-11): sc-398519的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG2a BP-HRP和m-IgGκ BP-HRP是Rock-2 Antibody (D-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Rock-2 Antibody (D-11) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

附加Rock抗体，包括Rock-1和Rock-2

Rock-2抗体（D-11）是一种IgG2a κ小鼠单克隆Rock-2抗体（也称为ROCK2抗体、Rho相关卷曲螺旋蛋白激酶2抗体），可通过WB、IP、IF和ELISA方法检测小鼠、大鼠和人类来源的Rock-2蛋白。Rock-2抗体（D-11）既有非偶联抗Rock-2抗体形式，也有多种偶联形式的抗Rock-2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。Ras相关的小GTP酶Rho负责调节基于肌动蛋白的细胞骨架结构，包括应力纤维、局部粘附和收缩环装置。Rho蛋白作为分子开关，能够开启和关闭细胞质分裂。尽管对Rho下游的信号传导知之甚少，但已经描述了许多假定的Rho效应蛋白，包括rhophilin、Rhotekin、citron和丝氨酸/苏氨酸激酶、蛋白激酶N。另外还描述了两种Rho激活的丝氨酸/苏氨酸激酶，分别命名为Rock-1和Rock-2（也称为Roka），为含Rho相关卷曲蛋白激酶。Rock-1和Rock-2与肌强直性营养不良激酶在结构上具有相似性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Rock-2 抗体 (D-11) 参考文献:

  1. 欧亚班诱导的细胞凋亡和 Rho 激酶：一个新的 caspase-2 裂解位点和 Rock-2 片段。  |  Ark, M., et al. 2010. Apoptosis. 15: 1494-506. PMID: 20661774
  2. ROCK-2 与成肌细胞迁移过程中的病灶粘附成熟有关。  |  Goetsch, KP., et al. 2014. J Cell Biochem. 115: 1299-307. PMID: 24700588
  3. 电针联合药物治疗对脑缺血大鼠纹状体RhoA/ROCK-2信号通路的影响。  |  Liu, M., et al. 2023. Brain Res Bull. 205: 110828. PMID: 38029846
  4. 硫化氢介导的ROCK2抑制对缺血性脑损伤具有血管保护作用。  |  Chen, Y., et al. 2024. Am J Physiol Cell Physiol.. PMID: 39716722
  5. 一种新型丝氨酸/苏氨酸激酶与 Ras 相关的 RhoA GTPase 结合，从而将激酶转运到外周膜。  |  Leung, T., et al. 1995. J Biol Chem. 270: 29051-4. PMID: 7493923
  6. 大鼠睾丸脂肪酸活化蛋白激酶（PKN）的纯化和表征。  |  Kitagawa, M., et al. 1995. Biochem J. 310 (Pt 2): 657-64. PMID: 7654208
  7. 蛋白激酶 N（PKN）和 PKN 相关蛋白 rhophilin 是小 GTPase Rho 的靶标。  |  Watanabe, G., et al. 1996. Science. 271: 645-8. PMID: 8571126
  8. 确定丝氨酸-苏氨酸激酶蛋白激酶 N 是 Rho 的假定靶标。  |  Amano, M., et al. 1996. Science. 271: 648-50. PMID: 8571127
  9. PKN氨基末端，N端独特基团对其酶活性的作用。  |  Kitagawa, M., et al. 1996. Biochem Biophys Res Commun. 220: 963-8. PMID: 8607876
  10. 小 GTP 结合蛋白 Rho 与一个与肌营养不良症激酶同源的 160 kDa Ser/Thr 蛋白激酶结合并激活该激酶。  |  Ishizaki, T., et al. 1996. EMBO J. 15: 1885-93. PMID: 8617235
  11. PKN 与神经丝蛋白的头棒结构域结合并使其磷酸化。  |  Mukai, H., et al. 1996. J Biol Chem. 271: 9816-22. PMID: 8621664
  12. RhoA 与 PKN 氨基末端，N 端之间相互作用的特征。  |  Shibata, H., et al. 1996. FEBS Lett. 385: 221-4. PMID: 8647255

订购信息

产品名称产品编号规格价格数量收藏夹

Rock-2 抗体 (D-11)

sc-398519
200 µg/ml
$316.00

Rock-2 (D-11): m-IgG Fc BP-HRP 套装

sc-530829
200 µg Ab; 10 µg BP
$354.00

Rock-2 (D-11): m-IgGκ BP-HRP 套装

sc-524375
200 µg Ab, 40 µg BP
$354.00

Rock-2 (D-11): m-IgG2a BP-HRP 套装

sc-547605
200 µg Ab; 10 µg BP
$354.00

Rock-2 抗体 (D-11) AC

sc-398519 AC
500 µg/ml, 25% agarose
$416.00

Rock-2 抗体 (D-11) HRP

sc-398519 HRP
200 µg/ml
$316.00

Rock-2 抗体 (D-11) FITC

sc-398519 FITC
200 µg/ml
$330.00

Rock-2 抗体 (D-11) PE

sc-398519 PE
200 µg/ml
$343.00

Rock-2 抗体 (D-11) Alexa Fluor® 488

sc-398519 AF488
200 µg/ml
$357.00

Rock-2 抗体 (D-11) Alexa Fluor® 546

sc-398519 AF546
200 µg/ml
$357.00

Rock-2 抗体 (D-11) Alexa Fluor® 594

sc-398519 AF594
200 µg/ml
$357.00

Rock-2 抗体 (D-11) Alexa Fluor® 647

sc-398519 AF647
200 µg/ml
$357.00

Rock-2 抗体 (D-11) Alexa Fluor® 680

sc-398519 AF680
200 µg/ml
$357.00

Rock-2 抗体 (D-11) Alexa Fluor® 790

sc-398519 AF790
200 µg/ml
$357.00

Has this antibody been used for WB/IF using neuroblastoma cells e.g. SK-N-SH?

Asked by: Vaani
Thank you for your question. It would be helpful if you could call us directly at 800-457-3801 ext. 1, allowing for a more interactive discussion of this and other related questions.
Answered by: Blake J
Date published: 2021-08-25

ROCK1 and ROCK2 exhibit 65% overall identity and 92% within the kinase domain. Can Rock-2 (D-11): sc-398519 monoclonal antibody distinguish human ROCK1 and ROCK2 in Western blot?

Asked by: CooperSen
Please contact Technical Service
Answered by: Tech Sales
Date published: 2021-05-12

Can Rock-2 (D-11): sc-398519 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: TinTin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Rock-2 (D-11): sc-398519 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24
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Rated 4 out of 5 by from WB ROCK2Used for detection by immunoblot of human ROCK1: sensitivity OK, low background
Date published: 2021-12-02
Rated 4 out of 5 by from Rock2 antibody for Western blottingsc-398519 works well for quantitative Western blot analysis of porcine renal proximal tubule cells.
Date published: 2019-04-06
Rated 4 out of 5 by from Very good in WB!!!I bought it 2 months ago! Works perfect in human HCC1954 cell line for at least 15 ug lysate!
Date published: 2018-02-01
Rated 4 out of 5 by from Très bon anticorpsJ'ai essayé cet anticorps la semaine dernière, il marche bien en Western Blot.
Date published: 2017-12-06
Rated 4 out of 5 by from OKit works well in WB ,but is not suitable for IP in NIH-3T3
Date published: 2017-11-22
Rated 5 out of 5 by from specific/sharp bands detectedThis antibody was tested on human aortic endothelial cells post various treatments. It gives specific band with no background.
Date published: 2017-09-14
Rated 5 out of 5 by from very good!I used this antibody for my WB experiment.It worked great and I got one clean WB band.
Date published: 2017-05-03
Rated 5 out of 5 by from Excellent antibody for WB in mouse kidneyI performed western blotting using mouse renal homogenate. The antibody works great and I was able to detect a strong band at 160 KDa. I am extremely happy that this antibody works very well
Date published: 2017-05-02
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Rock-2 抗体 (D-11) is rated 4.5 out of 5 by 14.
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