Date published: 2025-9-9
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PTEN Antibody (A2B1) - Western Blotting - Image 393454
PTEN 抗体 (A2B1) Alexa Fluor® 647: sc-7974 AF647. C3H/10T1/2 (A), HeLa (B) 和 MCF7 (C) 全细胞裂解液中 PTEN 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.
PTEN 抗体 (A2B1) Alexa Fluor® 647: sc-7974 AF647. C3H/10T1/2 (A), HeLa (B) 和 MCF7 (C) 全细胞裂解液中 PTEN 表达的直接荧光西部印迹分析. 用 UltraCruz® 阻断试剂: sc-516214 阻断. 用 Cruz Marker MW Tag-Alexa Fluor® 488 检测 Cruz Marker™ 分子量标准: sc-516790.

PTEN 抗体 (A2B1): sc-7974

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说明书
  • PTEN 抗体 A2B1 是小鼠单克隆 IgG1 κ，PTEN抗体, 在545篇文献中引用，规格为200 µg/ml
  • 免疫human来源PTEN位于C-terminus 的388-400氨基酸
  • PTEN 抗体 (A2B1) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 PTEN
  • 抗PTEN抗体（A2B1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取PTEN (A2B1): sc-7974的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 PTEN 抗体 (A2B1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与PTEN 抗体 (A2B1) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

PTEN抗体（A2B1）是一种小鼠单克隆IgG1 kappa轻链抗体，专门用于检测PTEN，也称为磷酸酶和Tensin同源物、MMAC1或TEP1。PTEN小鼠单克隆抗体（A2B1）靶向PTEN的C端表位，识别氨基酸388-400，对包括小鼠、大鼠和人类在内的多种物种均有效。PTEN (A2B1) 抗体可作为脂质和蛋白磷酸酶发挥作用，通过拮抗 PI3K/AKT 信号通路，在调节细胞生长、增殖和存活等过程方面发挥重要作用。PTEN 缺失或突变常见于多种癌症，包括胶质母细胞瘤、乳腺癌、肾癌和前列腺癌，这凸显了其在维持正常细胞功能和预防肿瘤发生方面的重要性。PTEN单克隆抗体（A2B1）用途广泛，适用于蛋白质印迹、免疫沉淀、免疫荧光、免疫组织化学（包括石蜡包埋样本）和酶联免疫吸附测定，是癌症研究和PTEN相关通路研究的重要工具。此外，PTEN (A2B1) 单克隆抗体有多种结合形式，如琼脂糖、HRP、PE、FITC和多种Alexa Fluor®结合物，可满足各种实验需求。PTEN小鼠单克隆抗体（A2B1）能够进行精确检测和分析，有助于加深对癌症发展和进展的分子机制以及靶向治疗潜力的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

PTEN 抗体 (A2B1) 参考文献:

  1. PTEN。  |  Worby, CA. and Dixon, JE. 2014. Annu Rev Biochem. 83: 641-69. PMID: 24905788
  2. 胶质瘤恶性阶段的克隆基因组改变。  |  James, CD., et al. 1988. Cancer Res. 48: 5546-51. PMID: 2901288
  3. PTEN：肿瘤抑制剂和代谢调节剂。  |  Chen, CY., et al. 2018. Front Endocrinol (Lausanne). 9: 338. PMID: 30038596
  4. 癌症中PTEN缺失的机制：一切皆因多样性。  |  Álvarez-Garcia, V., et al. 2019. Semin Cancer Biol. 59: 66-79. PMID: 30738865
  5. 基质中的PTEN。  |  Thies, KA., et al. 2019. Cold Spring Harb Perspect Med. 9: PMID: 31427286
  6. 染色质重塑中的PTEN。  |  Yang, J. and Yin, Y. 2020. Cold Spring Harb Perspect Med. 10: PMID: 31570373
  7. 恶性人类胶质瘤的特定染色体异常。  |  Bigner, SH., et al. 1988. Cancer Res. 48: 405-11. PMID: 3335011
  8. PTEN，一种在人类脑癌, 乳腺癌和前列腺癌中发生突变的假定蛋白酪氨酸磷酸酶基因。  |  Li, J., et al. 1997. Science. 275: 1943-7. PMID: 9072974
  9. 在染色体 10q23.3 上发现了一个在多种晚期癌症中发生突变的候选肿瘤抑制基因 MMAC1。  |  Steck, PA., et al. 1997. Nat Genet. 15: 356-62. PMID: 9090379
  10. 考登病（一种遗传性乳腺癌和甲状腺癌综合征）中 PTEN 基因的种系突变。  |  Liaw, D., et al. 1997. Nat Genet. 16: 64-7. PMID: 9140396
  11. 考登病患者中 PTEN/MMAC1 基因的种系突变。  |  Nelen, MR., et al. 1997. Hum Mol Genet. 6: 1383-7. PMID: 9259288
  12. PTEN 抑制胶质瘤细胞的生长需要功能性磷酸酶催化域。  |  Furnari, FB., et al. 1997. Proc Natl Acad Sci U S A. 94: 12479-84. PMID: 9356475

订购信息

产品名称产品编号规格价格数量收藏夹

PTEN 抗体 (A2B1)

sc-7974
200 µg/ml
$316.00

PTEN (A2B1): m-IgG Fc BP-HRP 套装

sc-528194
200 µg Ab; 10 µg BP
$354.00

PTEN (A2B1): m-IgGκ BP-HRP 套装

sc-520516
200 µg Ab, 40 µg BP
$354.00

PTEN (A2B1): m-IgG1 BP-HRP 套装

sc-542809
200 µg Ab; 20 µg BP
$354.00

PTEN 抗体 (A2B1) AC

sc-7974 AC
500 µg/ml, 25% agarose
$416.00

PTEN 抗体 (A2B1) HRP

sc-7974 HRP
200 µg/ml
$316.00

PTEN 抗体 (A2B1) FITC

sc-7974 FITC
200 µg/ml
$330.00

PTEN 抗体 (A2B1) PE

sc-7974 PE
200 µg/ml
$343.00

PTEN 抗体 (A2B1) Alexa Fluor® 488

sc-7974 AF488
200 µg/ml
$357.00

PTEN 抗体 (A2B1) Alexa Fluor® 546

sc-7974 AF546
200 µg/ml
$357.00

PTEN 抗体 (A2B1) Alexa Fluor® 594

sc-7974 AF594
200 µg/ml
$357.00

PTEN 抗体 (A2B1) Alexa Fluor® 647

sc-7974 AF647
200 µg/ml
$357.00

PTEN 抗体 (A2B1) Alexa Fluor® 680

sc-7974 AF680
200 µg/ml
$357.00

PTEN 抗体 (A2B1) Alexa Fluor® 790

sc-7974 AF790
200 µg/ml
$357.00

Hello, I want to know if you have any published figures with this antibody used for immunochemistry on human frozen brain tissue and on human FFPE brain tissue ? Many thanks in advance, Sincerely

Asked by: AnnaGabriellaICM
Thank you for your question. Here is a selection of relevant publications: PMIDs 19336457, 12904991, 15998902, 30287549.
Answered by: Tech Support Europe
Date published: 2023-01-16

Good morning. I used PTEN, sc7974 on prostate tissue. It gave me positive staining in extraglandular tissue and not in glandular structures. Could you guide me for proper steps to obtain immunostaining in the cells of prostatic glands? Thank you

Asked by: Nhelal
Thank you for your question. Please contact our Technical Service Department or your local distributor for assistance in troubleshooting your IHC reuslts with this antibody.
Answered by: Tech Service
Date published: 2019-09-26

Good Morning. I want to use PTEN monoclonal for IHC(P) on prostate tissue. I need your advice, Which product is more suitable: sc7974, sc133242, or sc393186? thanks

Asked by: Nhelal
Thank you for your question. All three of these PTEN antibodies are recommended for IHC(P) applications. PTEN Antibody (A2B1): sc-7974 is featured in over 300 publications, which you can find right here on the product page.
Answered by: Tech Support Europe
Date published: 2024-12-17

Hallo, when I tried to WB with BT-549(PTEN null as negative control) and Hela(Positive control), I find that non specific bands near 60 kDa in both BT-549 and Hela.  Also, in this datasheet, there are 3 bands in Hela. Which is tha authentic bands?

Asked by: taro333
Thank you for your question. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Tech Service
Date published: 2019-02-18

Hello, we are looking for PTEN Antibody to stained our sample which is goat ovary tissue in form of IHC-P. I wish you give me information that PTEN A2B1 (or any of your product) will be reactive to our sample. Thankyou.

Asked by: Dimas Reza
Thank you for your question. Unfortunately, we are not aware of any citations using this, or any of our other PTEN antibodies for detection in goat samples. Please contact our Technical Service Department with the protein sequence or accession number for the goat PTEN protein and we would be happy to help you perform sequence analysis to predict any antibodies that may cross-react with your species of interest.
Answered by: Technical Support
Date published: 2018-12-11

WB的稀释比例是多少

Asked by: 2333333
感谢您的提问。推荐用于WB的稀释度是1:100-1:1000，关于此问题的详细细节，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。 Thank you for your inquiry. The recommended dilution for WB is 1:100-1:1000. For more detailed information about this question, please contact our Asian Technical Service team at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 15
Date published: 2018-02-01

Good afternoon, Would it be possible to get the mentioned free 10 µg Sample of the anti-PTEN antibody PTEN (A2B1): sc-7974 ? Best regards, Ana Lopes

Asked by: Ana Lopes
Thank you for your question. Yes, we are happy to provide you with a free 10 µg sample of this antibody. Please call our European Support office toll-free at 00800 4573 8000, email europe@scbt.com, or contact us by live chat to request the free sample.
Answered by: Technical Support
Date published: 2017-07-28

I'll soon use Pten antibody for IF of Pten expression in mouse tissues so that I wonder if there's big difference between sc-7974 and sc-133197. Please advise.

Asked by: Jessica77
Thank you for your question. The main difference between PTEN (A2B1): sc-7974 and PTEN (B-1): sc-133197 is the immunogen sequence that they were raised against. Both antibodies are recommended for use in mouse tissue by IF. If you require further assistance, please contact Technical Service.
Answered by: Santa Cruz TS
Date published: 2017-05-19
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Rated 5 out of 5 by from 检索很方便我在查询需要的抗体，用起来比较方便。到底是买哪一种，还需要再斟酌斟酌。另外想申请个小的试用装，看看好不好用。
Date published: 2023-04-17
Rated 5 out of 5 by from ASSISTANT PROFESSORThis Ab give nice clean band on Western blotting. We are happy with this Ab.
Date published: 2021-06-30
Rated 4 out of 5 by from Very good for WBTested in SHSY5Y cell line at 1:1000 dilution in 5% BSA. Got 1 band at expected size.
Date published: 2021-05-05
Rated 4 out of 5 by from Good detectionI detected the bands but they were not very intense. Maybe it was due to the cell type (lung adenocarcinoma)
Date published: 2021-03-17
Rated 5 out of 5 by from Goodthis antibody works well for western blot with MEF
Date published: 2021-03-10
Rated 5 out of 5 by from good abGot this ab as a free sample, works very fine on mouse brain lysates. Clear cut band at the right molecular weight, extremely satisfied.
Date published: 2021-02-03
Rated 5 out of 5 by from Great antibodyYou can get great western blot images by using this antibody.
Date published: 2020-08-13
Rated 5 out of 5 by from good antibody for WBgood antibody for WB. PTEN shows up clear at where it should be.
Date published: 2019-09-23
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PTEN 抗体 (A2B1) is rated 4.7 out of 5 by 72.
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