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PPARα Antibody (H-2) - Western Blotting - Image 379499 — PPARα 抗体 (H-2): sc-398394. A-10 (A), U-87 MG (B), Hep G2 (C), NCI-H292 (D), HEL 92. 1. 7 (E) 和 AN3 CA (F) 全细胞裂解液中 PPARα 表达的 Western 印迹分析.

PPARα 抗体 (H-2): sc-398394. A-10 (A), U-87 MG (B), Hep G2 (C), NCI-H292 (D), HEL 92. 1. 7 (E) 和 AN3 CA (F) 全细胞裂解液中 PPARα 表达的 Western 印迹分析.

PPARα 抗体 (H-2): sc-398394

Name: PPAR&alpha;抗体 (H-2)
Brand: SCBT
SKU: sc-398394
Price: 316.0 USD
Availability: InStock

4.6(31)

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说明书

PPAR alpha 抗体 H-2 是小鼠单克隆 IgG_2a κ，PPARα抗体, 在174篇文献中引用，规格为200 µg/ml
免疫human物种的PPARα的氨基酸1-98
PPAR alpha 抗体 (H-2) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 PPARα
抗PPAR alpha抗体（H-2）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
可用于凝胶迁移和ChIP的TransCruz试剂(sc-398394 X, 200 µg/0.1 ml)
关于如何获取PPARα (H-2): sc-398394的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 2a BP-HRP">m-IgG_2a BP-HRP和m-IgGκ BP-HRP是PPARα Antibody (H-2) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与PPARα Antibody (H-2) 打包提供（请参阅下面的订购信息）。

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PPARα 抗体 (H-2) 参考文献:

PPARα的综合生理学和系统生物学。 | Kersten, S. 2014. Mol Metab. 3: 354-71. PMID: 24944896
天然产物靶向PPARα的潜力。 | Rigano, D., et al. 2017. Acta Pharm Sin B. 7: 427-438. PMID: 28752027
饮食过量通过肠道PPARα调节肠道上皮的吸收和表面。 | Stojanović, O., et al. 2021. Nat Commun. 12: 7031. PMID: 34857752
激活PPARα在眼部缺血性疾病中的治疗作用。 | Lee, D., et al. 2023. Histol Histopathol. 38: 391-401. PMID: 36305579
选择性激活PPARα可维持棕色脂肪细胞的产热能力。 | Egusa, G., et al. 2023. iScience. 26: 107143. PMID: 37456852
过氧化物酶体增殖激活受体（PPARs）的差异表达：成年大鼠 PPAR-α, -beta 和-gamma 的组织分布。 | Braissant, O., et al. 1996. Endocrinology. 137: 354-66. PMID: 8536636
过氧化物酶体增殖激活受体α基因的表达受到压力的刺激，并遵循昼夜节律。 | Lemberger, T., et al. 1996. J Biol Chem. 271: 1764-9. PMID: 8576180
多种 PPAR 异构体对脂肪生成的不同激活作用。 | Brun, RP., et al. 1996. Genes Dev. 10: 974-84. PMID: 8608944
大鼠肝脏中过氧化物酶体增殖激活受体-α mRNA 的调控。 | Sterchele, PF., et al. 1996. Arch Biochem Biophys. 326: 281-9. PMID: 8611035
孤儿核荷尔蒙受体 LXR alpha 与过氧化物酶体增殖激活受体相互作用，抑制过氧化物酶体增殖因子信号传导。 | Miyata, KS., et al. 1996. J Biol Chem. 271: 9189-92. PMID: 8621574
甲状腺激素和过氧化物酶体增殖物激活受体在调节过氧化物酶体增殖物反应基因中的相互关系 | Hunter, J., et al. 1996. Mol Cell Endocrinol. 116: 213-21. PMID: 8647322
过氧化物酶体增殖激活受体（PPAR）在小鼠结肠粘膜中的表达。 | Mansén, A., et al. 1996. Biochem Biophys Res Commun. 222: 844-51. PMID: 8651933

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
PPARα 抗体 (H-2)	sc-398394	200 µg/ml	$316.00
PPARα (H-2): m-IgG Fc BP-HRP 套装	sc-530783	200 µg Ab; 10 µg BP	$354.00
PPARα (H-2): m-IgGκ BP-HRP 套装	sc-524324	200 µg Ab, 40 µg BP	$354.00
PPARα (H-2): m-IgG_2a BP-HRP 套装	sc-547586	200 µg Ab; 10 µg BP	$354.00
PPARα 抗体 (H-2) X	sc-398394 X	200 µg/0.1 ml	$316.00
PPARα 抗体 (H-2) AC	sc-398394 AC	500 µg/ml, 25% agarose	$416.00
PPARα 抗体 (H-2) HRP	sc-398394 HRP	200 µg/ml	$316.00
PPARα 抗体 (H-2) FITC	sc-398394 FITC	200 µg/ml	$330.00
PPARα 抗体 (H-2) PE	sc-398394 PE	200 µg/ml	$343.00
PPARα 抗体 (H-2) Alexa Fluor^® 488	sc-398394 AF488	200 µg/ml	$357.00
PPARα 抗体 (H-2) Alexa Fluor^® 546	sc-398394 AF546	200 µg/ml	$357.00
PPARα 抗体 (H-2) Alexa Fluor^® 594	sc-398394 AF594	200 µg/ml	$357.00
PPARα 抗体 (H-2) Alexa Fluor^® 647	sc-398394 AF647	200 µg/ml	$357.00
PPARα 抗体 (H-2) Alexa Fluor^® 680	sc-398394 AF680	200 µg/ml	$357.00
PPARα 抗体 (H-2) Alexa Fluor^® 790	sc-398394 AF790	200 µg/ml	$357.00

In my experiments, I see multiple bands with this antibody, and a much stronger band above 55 kDa. In your pictures, I also see extra bands, but MWs aren’t specified. How do I know if these are real PTMs or non-specific?

Asked by: Moritz

Thank you for your question. For assistance please contact Technical Service. Please call 800-457-3801 or email scbt@scbt.com.

Answered by: BlakeJ

Date published: 2025-07-17

the antibody detects a strong signal larger than 55 kda in my cells. also double bands are shown in your example pictures. how do you explain these double bands, although the expected size of ppar alpha is 55 kda?

Asked by: Theresa

Thank you for your question. PPARα Antibody (H-2): sc-398394 is against amino acid range 1-98 of PPARα of human origin and will recognize any alternative splice variants possessing this particular sequence. Post-translational modifications of the protein will also contribute to its observed molecular weight in western blots.

Answered by: Tech Support Europe

Date published: 2025-06-04

请问这只抗体是否用knock out小鼠验证过，与PPARβ和PPARγ有交叉反应吗

Asked by: chen bangdang

感谢您的提问。如果您能直接拨打(+86) 021-60936350，或者发邮件到我们亚洲服务部的邮箱asia@scbio.cn我们就这个问题和其他相关问题进行更多的互动讨论，那将会很有帮助。 Thank you for your question.It would be helpful if you could call us directly at (+86) 021-60936350 or email our Asia Service department at asia@scbio.cn for more interactive discussions on this and other related issues.

Answered by: Zhanglong

Date published: 2025-01-13

Can antibody PPARa (H-2) 398384 be diluted in TBS-T?

Asked by: Simone

Thank you for your question. For applications that require blocking we recommend using UltraCruz® Blocking Reagent: sc-516214, milk TBS-T, or BSA in TBS-T as diluents. Otherwise, just TBS-T is suitable for diluting the antibody as well.

Answered by: Tech Support Europe

Date published: 2024-11-11

I want to ask you if i can get just 30 micro from PPAR alpha ??

Asked by: huda jw

Thank you for your question. Anti-PPARα Antibody (H-2): sc-398394 is available as 200 µg in 1 ml or as a sample with 10 µg in 50 µl. Please contact customercare to place an order for the former or reach out to our technical service team to arrange for a free sample of the antibody.

Answered by: Tech Support Europe

Date published: 2022-01-26

sc-39839多少钱一支

Asked by: 贝贝老师

Thank you for your question. It would be helpful if you could call us, allowing for a more interactive discussion of this and other related questions.

Answered by: Technical Support

Date published: 2021-07-23

Hi I need to stain PPar alpha on tissues from rabbit aortas for IHC. is this antibody good for it? Would it be better the unconiugated for or the HRP one? Thanks.

Asked by: Letizia

Thank you for your question. Please contact your local distributor or technical service for further assistance.

Answered by: Tech Service 1

Date published: 2021-01-28

I am processing Chromatin samples from mouse liver hepatocytes. I would like to know what would be the recommended dilution? I did not purchase the Trans Cruz antibody so starting with a diluted concentration.

Asked by: Sharlene5

Thank you for your question. Here is a link to the recommended protocol for ChIP: https://www.scbt.com/scbt/resources/protocols/chromatin-immunoprecipitation-assays We recommend using 0.2–2 µg of antibody for 100–500 µg supernatant .

Answered by: Technical Support

Date published: 2019-02-23

PPARα 抗体 (H-2) Questions - page 2

Rated 5 out of 5 by col77 from (H-2) X works well for EMSAThe H-2 X antibody works well for EMSA with huh-7 nuclear protein

Date published: 2023-06-28

Rated 5 out of 5 by huiruiyr from 条带完美实验结果挺满意的，条带挺完美的，还准备再试试别的实验，单克隆抗体效果就是好。下次还会继续选购santa产品，

Date published: 2022-02-08

Rated 4 out of 5 by JohnH from Good antibodyMultiple bands were detected but still expected size band was detected

Date published: 2021-12-03

Rated 5 out of 5 by Rosa from great antibodyVery specific antibody for Western Blot, no extra bands

Date published: 2021-07-06

Rated 4 out of 5 by paolam06 from buon funzionamentosono rimasta soddisfatta dell' anticorpo PPARα (H-2)

Date published: 2020-11-18

Rated 5 out of 5 by clarita from ottimo funzionamentoSoddisfatti dell'acquisto. L'anticorpo funziona molto bene.

Date published: 2020-11-06

Rated 4 out of 5 by MK56 from GreatThe antibody is worked well. I`m happy with the results.

Date published: 2020-06-08

Rated 5 out of 5 by Anonymous from Good workThe antibody worked very well in our previous experiment for IHC staining.

Date published: 2019-10-17

PPARα 抗体 (H-2) Reviews - page 2

PPARα 抗体 (H-2) is rated 4.6 out of 5 by 31.

Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。

种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	PPARA	Entrez Gene ID: 5465	22q13.31	XM_005261655, XM_005261656, XM_006724269, XM_006724270, XM_011530239, XM_011530240, XM_011530241, XM_011530242, XM_011530243, XM_011530244, XM_011530245, XM_017028839, XM_017028840, NM_001001928, NM_005036, NM_001001929, NM_001001930, NM_032644	UniProt ID: Q07869	170998
Mouse	Ppara	Entrez Gene ID: 19013	15 E2	XM_006520619, XM_006520620, XM_006520621, XM_006520622, XM_006520623, XM_006520624, XM_011245516, XM_011245517, XM_011245519, NM_001113418, NM_011144	UniProt ID: P23204	N/A

PPARα 抗体 (H-2): sc-398394

PPARα 抗体 (H-2) 参考文献:

PPARα 抗体 (H-2) 规格

替代产品名称: PPAR alpha

产品克隆名称: H-2

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG2a kappa light chain

氨基酸: 1-98

抗原表位靶标: PPARα

抗原表位种属: human

产品反应性/检测: PPARα

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 174篇各种科学出版物的引文

PPARα 抗体 (H-2)

PPARα (H-2): m-IgG Fc BP-HRP 套装

PPARα (H-2): m-IgGκ BP-HRP 套装

PPARα (H-2): m-IgG2a BP-HRP 套装

PPARα 抗体 (H-2) X

PPARα 抗体 (H-2) AC

PPARα 抗体 (H-2) HRP

PPARα 抗体 (H-2) FITC

PPARα 抗体 (H-2) PE

PPARα 抗体 (H-2) Alexa Fluor® 488

PPARα 抗体 (H-2) Alexa Fluor® 546

PPARα 抗体 (H-2) Alexa Fluor® 594

PPARα 抗体 (H-2) Alexa Fluor® 647

PPARα 抗体 (H-2) Alexa Fluor® 680

PPARα 抗体 (H-2) Alexa Fluor® 790

In my experiments, I see multiple bands with this antibody, and a much stronger band above 55 kDa. In your pictures, I also see extra bands, but MWs aren&rsquo;t specified. How do I know if these are real PTMs or non-specific?

the antibody detects a strong signal larger than 55 kda in my cells. also double bands are shown in your example pictures. how do you explain these double bands, although the expected size of ppar alpha is 55 kda?

请问这只抗体是否用knock out小鼠验证过，与PPAR&beta;和PPAR&gamma;有交叉反应吗

Can antibody PPARa (H-2) 398384 be diluted in TBS-T?

I want to ask you if i can get just 30 micro from PPAR alpha ??

sc-39839多少钱一支

Hi I need to stain PPar alpha on tissues from rabbit aortas for IHC. is this antibody good for it? Would it be better the unconiugated for or the HRP one? Thanks.

I am processing Chromatin samples from mouse liver hepatocytes. I would like to know what would be the recommended dilution? I did not purchase the Trans Cruz antibody so starting with a diluted concentration.

抗体同型: IgG_2a kappa light chain

瓶的信息: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

PPARα (H-2): m-IgG_2a BP-HRP 套装

PPARα 抗体 (H-2) Alexa Fluor^® 488

PPARα 抗体 (H-2) Alexa Fluor^® 546

PPARα 抗体 (H-2) Alexa Fluor^® 594

PPARα 抗体 (H-2) Alexa Fluor^® 647

PPARα 抗体 (H-2) Alexa Fluor^® 680

PPARα 抗体 (H-2) Alexa Fluor^® 790

In my experiments, I see multiple bands with this antibody, and a much stronger band above 55 kDa. In your pictures, I also see extra bands, but MWs aren’t specified. How do I know if these are real PTMs or non-specific?

请问这只抗体是否用knock out小鼠验证过，与PPARβ和PPARγ有交叉反应吗