PFKFB4 Antibody (A-1): sc-514792

PFK-2 tes Antibody (A-1) is a mouse monoclonal IgG3 kappa light chain antibody that detects PFK-2 tes of human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-PFK-2 tes antibody (A-1) is available in a non-conjugated form, specifically designed for versatile applications in research. PFK-2 tes, also known as PFKFB4 (6-phosphofructo-2-kinase/fructose-2,6-biphosphatase 4), is a 469 amino acid cytoplasmic enzyme that plays a crucial role in glycolysis regulation by synthesizing and degrading fructose 2,6-bisphosphate, a potent allosteric activator of phosphofructokinase-1. PFK-2 tes is primarily expressed in the testis and functions as a homodimer, with activity modulated through phosphorylation. Notably, PFK-2 tes expression increases under hypoxic conditions via a hypoxia-inducible factor 1-alpha (HIF-1α) dependent mechanism, highlighting PFK-2 tes importance in cellular metabolism during low oxygen availability. Elevated levels of PFK-2 tes have been observed in various cancer cell lines, suggesting PFK-2 tes involvement in the Warburg effect, where cancer cells preferentially utilize glycolysis for ATP production even in the presence of oxygen. This metabolic shift supports rapid cell proliferation and survival in tumor microenvironments, making PFK-2 tes a potential target for therapeutic intervention in cancer metabolism.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

PFKFB4 Antibody (A-1) References:

1. Cloning, expression and chromosomal localization of a human testis 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase gene.  |  Manzano, A., et al. 1999. Gene. 229: 83-9. PMID: 10095107
2. Specific expression of pfkfb4 gene in spermatogonia germ cells and analysis of its 5'-flanking region.  |  Gómez, M., et al. 2005. FEBS Lett. 579: 357-62. PMID: 15642344
3. Overexpression of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4 in the human breast and colon malignant tumors.  |  Minchenko, OH., et al. 2005. Biochimie. 87: 1005-10. PMID: 15925437
4. Expression and hypoxia-responsiveness of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase 4 in mammary gland malignant cell lines.  |  Minchenko, OH., et al. 2005. Acta Biochim Pol. 52: 881-8. PMID: 16025159
5. Splice isoform of 6-phosphofructo-2-kinase/fructose-2,6-bisphosphatase-4: expression and hypoxic regulation.  |  Minchenko, OH., et al. 2005. Mol Cell Biochem. 280: 227-34. PMID: 16311927
6. Hypoxic regulation of PFKFB-3 and PFKFB-4 gene expression in gastric and pancreatic cancer cell lines and expression of PFKFB genes in gastric cancers.  |  Bobarykina, AY., et al. 2006. Acta Biochim Pol. 53: 789-99. PMID: 17143338
7. Nuclear targeting of 6-phosphofructo-2-kinase (PFKFB3) increases proliferation via cyclin-dependent kinases.  |  Yalcin, A., et al. 2009. J Biol Chem. 284: 24223-32. PMID: 19473963
8. Cloning of cDNA encoding for a novel isozyme of fructose 6-phosphate, 2-kinase/fructose 2,6-bisphosphatase from human placenta.  |  Sakai, A., et al. 1996. J Biochem. 119: 506-11. PMID: 8830046

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-PFKFB4 Antibody (A-1) has 0 citations in a variety of scientific publications.

Primary antibodies like Anti-PFKFB4 Antibody (A-1) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

PFKFB4 Antibody (A-1) Specifications

Alternate Product Name: PFKFB4

Product Clone Name: A-1

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG₃ kappa light chain

Amino Acids: 181-196

Epitope Target: PFKFB4

Epitope Species: human

Product Reactivity/Detection: PFKFB4

Species Reactivity/Detection: human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG₃ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 0 citations in various scientific publications

Available Conjugates/Forms for PFKFB4 Antibody (A-1): P: Blocking Peptide