Date published: 2025-9-7
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Parkin Antibody (PRK8) - Western Blotting - Image 388249
Parkin 抗体 (PRK8): sc-32282. 大鼠脑组织提取物中 Parkin 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 680: sc-516180.
Parkin 抗体 (PRK8): sc-32282. 大鼠脑组织提取物中 Parkin 表达的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 使用的检测试剂: m-IgGκ BP-CFL 680: sc-516180.

Parkin 抗体 (PRK8): sc-32282

4.5(48)
寫評論提問

说明书
  • Parkin 抗体 PRK8 是小鼠单克隆 IgG2b κ，Parkin抗体, 在319篇文献中引用，规格为200 µg/ml
  • 针对重组human Parkin
  • Parkin 抗体 (PRK8) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 Parkin
  • 抗Parkin抗体（PRK8）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Parkin (PRK8): sc-32282的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 Parkin 抗体 (PRK8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Parkin 抗体 (PRK8) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

Parkin抗体（PRK8）是一种小鼠单克隆IgG2b kappa轻链抗体，靶向Parkin，也称为PRKN、PARK2或parkin RBR E3泛素蛋白连接酶，已证实对人类、小鼠和大鼠样本有反应。Parkin是由PARK2基因编码的465个氨基酸的E3泛素连接酶，对于泛素化过程至关重要，泛素化过程会标记蛋白质以使其降解，通过消除受损或错误折叠的蛋白质来维持细胞内环境稳定。Parkin在线粒体自噬中尤为重要，线粒体自噬是指选择性自噬去除功能失调的线粒体，这对于保持线粒体的质量和功能至关重要。PARK2基因的突变与常染色体隐性遗传性青少年帕金森病有关，这凸显了Parkin在神经元健康中的重要性。抗Parkin抗体（PRK8）是针对重组人Parkin的，靶向蛋白质C端区域内的表位。Parkin (PRK8) 单克隆抗体可用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）。Parkin 在大脑各区域的神经元中均有表达，包括基底神经节、中脑、小脑和大脑皮层，其功能在物种间进化上保持不变。抗帕金抗体（PRK8）是研究帕金功能及其在细胞过程和神经退行性疾病研究中的参与情况的重要工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Parkin 抗体 (PRK8) 参考文献:

  1. 人脑和外周白细胞中 parkin 基因的差异表达。  |  Sunada, Y., et al. 1998. Neurosci Lett. 254: 180-2. PMID: 10214987
  2. Parkin通过其E3泛素蛋白连接酶活性抑制折叠蛋白应激诱导的细胞死亡。  |  Imai, Y., et al. 2000. J Biol Chem. 275: 35661-4. PMID: 10973942
  3. 在神经细胞和非神经细胞中，Parkin 与肌动蛋白丝相关联。  |  Huynh, DP., et al. 2000. Ann Neurol. 48: 737-44. PMID: 11079537
  4. 成年小鼠大脑中 Parkin 的表达  |  Stichel, CC., et al. 2000. Eur J Neurosci. 12: 4181-94. PMID: 11122330
  5. 脊椎动物和无脊椎动物大脑中 Parkin 蛋白的免疫检测：使用特异性抗体的比较研究。  |  Horowitz, JM., et al. 2001. J Chem Neuroanat. 21: 75-93. PMID: 11173222
  6. 帕金森和帕金森病  |  Mizuno, Y., et al. 2001. Curr Opin Neurol. 14: 477-82. PMID: 11470964
  7. Parkin 的 E3 泛素蛋白连接酶活性依赖于 RING 手指（TRIAD）元件的协同作用。  |  Rankin, CA., et al. 2001. J Biomed Sci. 8: 421-9. PMID: 11549885

订购信息

产品名称产品编号规格价格数量收藏夹

Parkin 抗体 (PRK8)

sc-32282
200 µg/ml
$316.00

Parkin (PRK8): m-IgG Fc BP-HRP 套装

sc-528453
200 µg Ab; 10 µg BP
$354.00

Parkin (PRK8): m-IgGκ BP-HRP 套装

sc-520834
200 µg Ab, 40 µg BP
$354.00

Parkin (PRK8): m-IgG2b BP-HRP 套装

sc-550038
200 µg Ab; 10 µg BP
$354.00

Parkin 抗体 (PRK8) AC

sc-32282 AC
500 µg/ml, 25% agarose
$416.00

Parkin 抗体 (PRK8) HRP

sc-32282 HRP
200 µg/ml
$316.00

Parkin 抗体 (PRK8) FITC

sc-32282 FITC
200 µg/ml
$330.00

Parkin 抗体 (PRK8) PE

sc-32282 PE
200 µg/ml
$343.00

Parkin 抗体 (PRK8) Alexa Fluor® 488

sc-32282 AF488
200 µg/ml
$357.00

Parkin 抗体 (PRK8) Alexa Fluor® 546

sc-32282 AF546
200 µg/ml
$357.00

Parkin 抗体 (PRK8) Alexa Fluor® 594

sc-32282 AF594
200 µg/ml
$357.00

Parkin 抗体 (PRK8) Alexa Fluor® 647

sc-32282 AF647
200 µg/ml
$357.00

Parkin 抗体 (PRK8) Alexa Fluor® 680

sc-32282 AF680
200 µg/ml
$357.00

Parkin 抗体 (PRK8) Alexa Fluor® 790

sc-32282 AF790
200 µg/ml
$357.00

What % of milk to you recommend? I get a lot of background in 1% milk and it is hard to tell where the band is. Is it ok to boil samples? Thank you!

Asked by: rml294
Thank you for your question. We recommend 5% milk in TBST and boiling samples 3-5 minutes at 95 degrees.
Answered by: BlakeJ
Date published: 2023-06-14

I am trying to find out some good IF images in a reputed journal. Although in the reference you have mentioned in 2 Sci Advance journal. Unfortunately, I haven't found any IF-image in those papers. Do you have some good reference images?

Asked by: Sanjuumass01
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-03-13

Which is the immunogen of anti-Parkin SC-32282?

Asked by: ElenaM
Thank you for your question. SC-32282 was raised against amino acids 399-465 of human Parkin.
Answered by: Tech Support Europe
Date published: 2022-05-10

Hello. I have tried the parkin antibody at 1:50, 1:100, 1:200 dilutions for immunofluorescent staining using retinal cryosections and agar-embedded vibratome slices with no detectable signal. Do you have any recommendations for troubleshooting? Thanks.

Asked by: tshetty
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-02-09

Hallo, wir würden gerne den Parkin Antikörper (PRK8, sc-32282) ein mal testen. Wäre es möglich eine Probegröße davon zu bekommen? Vielen Dank im Voraus.

Asked by: Anonymous
Vielen Dank für Ihre Anfrage. Bitte kontaktieren Sie unseren Technischen Service für ein Sample dieses Antikörpers.
Answered by: Tech Support Europe
Date published: 2020-09-07

Hi. I am using this antibody to detect parkin in my cells (SH-SY5Y cells) via ICC. However, I am not able to detect any signal (I have used up to 1:50 dilution and have used both milk and bsa). Could you possibly give me an optimal protocol for ICC?

Asked by: Penn
Thank you for your question. A link to our recommended protocol is provided below. A dilution of 1:50 sounds appropriate. Please contact our Technical Service Department and we would be happy to help you troubleshoot this antibody. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2019-02-22

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: Cweed
Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. SC-32282 is currently available conjugated to FITC, AF488, and AF647.
Answered by: TechService7
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary for IHC?

Asked by: Cweed
We recommend using one of our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-15
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Rated 5 out of 5 by from Works well for WBWorks well on neonatal mouse lungs at concentration of 30ug. Ab dilution will be 1:500 for overnight @ 4oC.
Date published: 2024-12-10
Rated 4 out of 5 by from Great for WBIt is a good antibody in WB. It works in dilution 1:1000.
Date published: 2024-03-20
Rated 2 out of 5 by from Does not work for IF.This product works for WB nicely. However, IF does not work.
Date published: 2023-10-18
Rated 5 out of 5 by from Great for WBProvides a strong signal for WB (tested in human cell lines, 1:1000 dilution).
Date published: 2023-10-15
Rated 5 out of 5 by from 結果拍出來很漂亮用在神經細胞實驗上跑western blotting，在對的分子量上，終於拍出漂亮的結果超級開心~~
Date published: 2023-05-17
Rated 4 out of 5 by from 条带明显应用在BV-2细胞上WB的检测PARKIN，在目标位置的条带很明显，没有杂带，同时背景很干净，推荐购买。
Date published: 2022-07-26
Rated 5 out of 5 by from Good for cyto-stainningI got nice immunostaining images. For rat-cardiomyoblast, H9c2. I used it 1:100.
Date published: 2022-06-01
Rated 5 out of 5 by from Excellent functionI bought this product a week ago. It has excellent functions. The experiment is going well. I will continue to support Santa
Date published: 2021-11-24
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Parkin 抗体 (PRK8) is rated 4.5 out of 5 by 48.
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