p130 Antibody (F-8): sc-365163
- p130 Antibody (F-8) is a mouse monoclonal IgG1 κ provided at 200 µg/ml
- specific for an epitope mapping between amino acids 1115-1139 at the C-terminus of p130raised against a peptide mapping at the C-terminus of p130 of human origin of human origin
- recommended for detection of p130 of mouse, rat and human origin by WB, IP, IF and ELISA; also reactive with additional species, including and canine, bovine and porcine
- TransCruz reagent for ChIP application (sc-365163 X, 200 µg/0.1 ml)
- m-IgG Fc BP-HRP, m-IgG1 BP-HRP and m-IgGκ BP-HRP are the preferred secondary detection reagents for p130 Antibody (F-8) for WB applications. These reagents are now offered in bundles with p130 Antibody (F-8) (see ordering information below).
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SEE ALSO...
p130 Antibody (F-8) is a mouse monoclonal IgG1 antibody that detects p130 in mouse, rat, and human samples through applications such as western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). p130, a member of the retinoblastoma (Rb) protein family, plays a crucial role in regulating the cell cycle and maintaining cellular quiescence. p130 is primarily located in the nucleus, where p130 interacts with E2F transcription factors to inhibit their activity, thereby preventing the transcription of genes required for cell cycle progression. This nuclear localization is vital for p130′s function, as nuclear localization allows p130 to effectively control the transition from the G0 phase to the G1 phase of the cell cycle, ensuring that cells do not prematurely enter the cycle and proliferate uncontrollably. Additionally, p130 undergoes specific post-translational modifications, including phosphorylation on serine and threonine residues, which are essential for regulatory functions and are particularly important during cellular responses to growth signals. Anti-p130 antibody (F-8) is an invaluable tool for researchers studying cell cycle regulation and the mechanisms underlying cellular proliferation in various biological contexts.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
p130 Antibody (F-8) References:
- Nucleocytoplasmic shuttling of p130/RBL2: novel regulatory mechanism. | Chestukhin, A., et al. 2002. Mol Cell Biol. 22: 453-68. PMID: 11756542
- pRb2/p130 decreases sensitivity to apoptosis induced by camptothecin and doxorubicin but not by taxol. | Tonini, T., et al. 2004. Clin Cancer Res. 10: 8085-93. PMID: 15585644
- Increased expression of p130 in Alzheimer disease. | Previll, LA., et al. 2007. Neurochem Res. 32: 639-44. PMID: 17006760
- Acetylation of retinoblastoma like protein2 (Rb2/p130) in tumor tissues. | Khan, ZN., et al. 2013. Asian Pac J Cancer Prev. 14: 2255-8. PMID: 23725122
- RBL2/p130 is a direct AKT target and is required to induce apoptosis upon AKT inhibition in lung cancer and mesothelioma cell lines. | Pentimalli, F., et al. 2018. Oncogene. 37: 3657-3671. PMID: 29606701
- Proteasomal degradation of p130 facilitate cell cycle deregulation and impairment of cellular differentiation in high-risk Human Papillomavirus 16 and 18 E7 transfected cells. | Gandhi, S., et al. 2021. Mol Biol Rep. 48: 5121-5133. PMID: 34169395
- Cyclin F drives proliferation through SCF-dependent degradation of the retinoblastoma-like tumor suppressor p130/RBL2. | Enrico, TP., et al. 2021. Elife. 10: PMID: 34851822
- Functional analysis of pRb2/p130 interaction with cyclins. | Claudio, PP., et al. 1996. Cancer Res. 56: 2003-8. PMID: 8616840
- pRB, p107 and p130 as transcriptional regulators: role in cell growth and differentiation. | Mayol, X. and Graña, X. 1997. Prog Cell Cycle Res. 3: 157-69. PMID: 9580269
- Role of the retinoblastoma protein family, pRB, p107 and p130 in the negative control of cell growth. | Graña, X., et al. 1998. Oncogene. 17: 3365-83. PMID: 9916999
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
p130 Antibody (F-8) | sc-365163 | 200 µg/ml | $322.00 | |||
p130 Antibody (F-8): m-IgG Fc BP-HRP Bundle | sc-537783 | 200 µg Ab; 10 µg BP | $361.00 | |||
p130 Antibody (F-8): m-IgGκ BP-HRP Bundle | sc-535167 | 200 µg Ab; 40 µg BP | $361.00 | |||
p130 Antibody (F-8): m-IgG1 BP-HRP Bundle | sc-545349 | 200 µg Ab; 20 µg BP | $361.00 | |||
p130 (F-8) Neutralizing Peptide | sc-365163 P | 100 µg/0.5 ml | $69.00 | |||
p130 Antibody (F-8) X | sc-365163 X | 200 µg/0.1 ml | $322.00 |