Date published: 2025-9-6

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p-PFK-2/PFKFB2 抗体 (B-3): sc-373806

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说明书
  • p-PFK-2/PFKFB2抗体(B-3)是小鼠单克隆IgG1 κ, 在1篇文献中引用,规格为200 µg/ml
  • 抗-p-PFK-2/PFKFB2 抗体 (B-3) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Ser 483 phosphorylated PFKFB2; 也和以下物种反应,包括: 和 equine, canine and bovine
  • 抗p-PFK-2/PFKFB2抗体(B-3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取p-PFK-2/PFKFB2 (B-3): sc-373806免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是p-PFK-2/PFKFB2 Antibody (B-3) for WB applications. 的首选辅助检测试剂,这些试剂现与p-PFK-2/PFKFB2 Antibody (B-3) 打包提供(请参阅下面的订购信息)。

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関連項目

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-PFK-2/PFKFB2 抗体 (B-3) 参考文献:

  1. 6-磷酸果糖-2-激酶的诱导型基因产物具有富含 AU 的不稳定元素:在肿瘤细胞糖酵解和沃伯格效应中发挥作用。  |  Chesney, J., et al. 1999. Proc Natl Acad Sci U S A. 96: 3047-52. PMID: 10077634
  2. 白色脂肪组织中 6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶同工酶的表达和调控  |  Bruni, P., et al. 1999. Eur J Biochem. 259: 756-61. PMID: 10092861
  3. 胰岛素对心脏 6-磷酸果糖-2-激酶的激活来自于 Ser-466 和 Ser-483 磷酸化,需要 3-磷酸肌醇依赖性激酶-1,但不需要蛋白激酶 B。  |  Bertrand, L., et al. 1999. J Biol Chem. 274: 30927-33. PMID: 10521487
  4. 大鼠脑6-磷酸果糖2-激酶/2,6-果糖二磷酸酶的组织特异性替代剪接。  |  Watanabe, F. and Furuya, E. 1999. FEBS Lett. 458: 304-8. PMID: 10570929
  5. 磷酸激酶激活蛋白激酶2(AMPK)对心脏磷酸激酶2(PFK-2)的磷酸化激活在缺血期间对糖酵解的刺激中发挥作用。  |  Marsin, AS., et al. 2000. Curr Biol. 10: 1247-55. PMID: 11069105
  6. 死后早期AMP激活蛋白激酶(AMPK)激活导致猪长肌中磷酸果糖激酶-2和-1(PFK-2和PFK-1)磷酸化,并出现苍白、柔软和渗出(PSE)症状。  |  Shen, QW., et al. 2006. J Agric Food Chem. 54: 5583-9. PMID: 16848549
  7. 大鼠肝癌细胞中 6-磷酸果糖-2-激酶/果糖-2,6-二磷酸酶基因表达的激素控制  |  Cifuentes, ME., et al. 1991. J Biol Chem. 266: 1557-63. PMID: 1846360
  8. 核糖激酶家族进化以及大肠杆菌PfkB亚家族代表Pfk-2活性位点保守残基的作用。  |  Cabrera, R., et al. 2010. Arch Biochem Biophys. 502: 23-30. PMID: 20599671
  9. 编码果糖-6-P,2-激酶:果糖-2,6-二磷酸酶的人类肝脏 cDNA 的分子克隆, 序列分析和表达。  |  Algaier, J. and Uyeda, K. 1988. Biochem Biophys Res Commun. 153: 328-33. PMID: 2837207
  10. RSK调节PFK-2活性,促进黑色素瘤的代谢重整。  |  Houles, T., et al. 2018. Cancer Res. 78: 2191-2204. PMID: 29440170
  11. 人静止成纤维细胞在血清刺激下的糖酵解率增加。2,6-二磷酸果糖的可能作用。  |  Bruni, P., et al. 1983. FEBS Lett. 159: 39-42. PMID: 6223840
  12. 人胎盘型 6-磷酸果糖-2-激酶/2,6-果糖二磷酸酶在各种细胞和细胞系中的表达。  |  Hirata, T., et al. 1998. Biochem Biophys Res Commun. 242: 680-4. PMID: 9464277

订购信息

产品名称产品编号规格价格数量收藏夹

p-PFK-2/PFKFB2 抗体 (B-3)

sc-373806
200 µg/ml
$316.00

p-PFK-2 car (B-3): m-IgG Fc BP-HRP 套装

sc-527415
200 µg Ab; 10 µg BP
$354.00

p-PFK-2 car (B-3): m-IgG1 BP-HRP 套装

sc-532788
200 µg Ab; 20 µg BP
$354.00

p-PFK-2 car 抗体 (B-3) AC

sc-373806 AC
500 µg/ml, 25% agarose
$416.00

p-PFK-2 car 抗体 (B-3) HRP

sc-373806 HRP
200 µg/ml
$316.00

p-PFK-2 car 抗体 (B-3) FITC

sc-373806 FITC
200 µg/ml
$330.00

p-PFK-2 car 抗体 (B-3) PE

sc-373806 PE
200 µg/ml
$343.00

p-PFK-2 car 抗体 (B-3) Alexa Fluor® 488

sc-373806 AF488
200 µg/ml
$357.00

p-PFK-2 car 抗体 (B-3) Alexa Fluor® 546

sc-373806 AF546
200 µg/ml
$357.00

p-PFK-2 car 抗体 (B-3) Alexa Fluor® 594

sc-373806 AF594
200 µg/ml
$357.00

p-PFK-2 car 抗体 (B-3) Alexa Fluor® 647

sc-373806 AF647
200 µg/ml
$357.00

p-PFK-2 car 抗体 (B-3) Alexa Fluor® 680

sc-373806 AF680
200 µg/ml
$357.00

p-PFK-2 car 抗体 (B-3) Alexa Fluor® 790

sc-373806 AF790
200 µg/ml
$357.00

p-PFK-2 car (B-3) 中和勝肽

sc-373806 P
100 µg/0.5 ml
$68.00

What application is the blocking peptide sc-373806 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from it worked so good.I used it in WB of samples from human cultured cells, and the signal could be detected easily with clear background.
Date published: 2022-08-22
Rated 5 out of 5 by from Produced nice Western blot data of PFKProduced nice Western blot data of PFK-2 car phosphorylation in non-transfected, untreated mouse PFK-2 car transfected and lambda protein phosphatase treated mouse PFK-2 car transfected 293T whole cell lysates -SCBT QC
Date published: 2013-11-30
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p-PFK-2/PFKFB2 抗体 (B-3) is rated 5.0 out of 5 by 2.
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