p-HSP 27抗体 (5B9) 推荐用于 mouse, rat, human 和 canine 来源的Ser 82 phosphorylated HSP 27 WB 和 IP检测

p-HSP 27 抗体 (5B9): sc-81498

p-HSP 27抗体 (5B9) is rated 4.0 out of 5 by 2.
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说明书

    • p-HSP 27 抗体 5B9 是小鼠单克隆 IgG2a, 在2篇文献中引用,提供 50 µg/0.5 ml
    • raised against phosphopeptide corresponding to amino acid residues surrounding serine 82 of HSP 27 of human origin
    • 推荐用于 mouse, rat, human 和 canine 来源的Ser 82 phosphorylated HSP 27 WB 和 IP检测; 不会与 non-phosphorylated HSP 27 or unrelated p-Ser proteins产生交联反应

I am using p-HSP 27 (5B9): sc-81498 antibody for immunoprecipitation, how much antibody should I use?

Asked by: jerojero
Thank you for your question. We recommend using 1–10 µl (i.e., 0.2–2 µg) of antibody with 1 ml of cell lysate. However, optimal antibody concentration should be determined by titration. Please find our full immunoprecipitation protocol here: https://www.scbt.com/scbt/resources/protocols/immunoprecipitation-western-blots
Answered by: Technical Support
Date published: 2017-03-24
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Rated 4 out of 5 by from Reduction in signal supporting specificity Reduction in signal supporting specificity via western blot analysis of HSP 27 phosphorylation in serum starved Hep G2 cells and serum starved Hep G2 cells treated with 10 ng/ml EGF for 5 min, 15 min, 30 min, 1 hr, 2 hrs, 4 hrs and 8 hrs. -SCBT QC
Date published: 2015-03-22
Rated 4 out of 5 by from Supporting western blot analysis of HSP 27 Supporting western blot analysis of HSP 27 phosphorylation in non-transfected 293T, mouse HSP 27 transfected 293T and ECV304 whole cell lysates. -SCBT QC
Date published: 2015-01-25
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