Date published: 2025-9-7
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MMP-3 Antibody (1B4) - Western Blotting - Image 141328
MMP-3 抗体 (1B4): sc-21732. HT-1080 (A), ECV304 (B), HUV-EC-C (C), WI-38 (D), BJ (E) 和 CCD-1064SK (F) 全细胞裂解液中 MMP-3 表达的 Western 印迹分析.
MMP-3 抗体 (1B4): sc-21732. HT-1080 (A), ECV304 (B), HUV-EC-C (C), WI-38 (D), BJ (E) 和 CCD-1064SK (F) 全细胞裂解液中 MMP-3 表达的 Western 印迹分析.

MMP-3 抗体 (1B4): sc-21732

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寫評論提問

说明书
  • MMP-3 抗体 1B4 是小鼠单克隆 IgG1，MMP-3抗体, 在74篇文献中引用，规格为200 µg/ml
  • 免疫human物种的MMP-3的氨基酸317-327
  • MMP-3 抗体 (1B4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 MMP-3
  • 抗MMP-3抗体（1B4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MMP-3 (1B4): sc-21732的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 MMP-3 抗体 (1B4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MMP-3 抗体 (1B4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MMP 抗体 包括 MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-2, MMP-21, MMP-26, MMP-3和MMP-7

MMP-3抗体（1B4）是一种小鼠单克隆IgG1抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和免疫组织化学检测小鼠、大鼠和人类来源的MMP-3蛋白。抗MMP-3抗体（1B4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。基质金属蛋白酶（MMPs）是肽酶家族中一个至关重要的成员，在细胞外基质成分（如胶原蛋白、明胶、纤维粘连蛋白、层粘连蛋白和蛋白聚糖）的降解中发挥着重要作用，而这些成分对于组织重塑和修复至关重要。MMP-3（又称基质金属蛋白酶-1）尤其重要，因为它不仅激活原胶原酶，还参与其他MMP的加工，从而影响各种生理和病理过程，包括伤口愈合、炎症和癌症进展。MMP-3的激活受到严格调控，可通过两种不同的途径发生：一种是由MMP-3自身缓慢直接激活，另一种是与组织或血浆蛋白酶结合快速激活。了解MMP-3的功能至关重要，因为MMP-3失调与多种疾病有关，包括关节炎和癌症，这使得MMP-3成为治疗干预和研究的重要靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MMP-3 抗体 (1B4) 参考文献:

  1. 乳腺癌基质细胞中特异表达的新型金属蛋白酶基因  |  Basset, P., et al. Nature. 348: 699-704. PMID: 1701851
  2. 基质金属蛋白酶 3（stromelysin）激活组织胶原酶的机制。  |  Suzuki, K., et al. 1990. Biochemistry. 29: 10261-70. PMID: 2176865
  3. 人类基质金属蛋白酶-3 的完整一级结构。与stromelysin相同。  |  Saus, J., et al. 1988. J Biol Chem. 263: 6742-5. PMID: 3360803
  4. Matrix metalloproteinase-3 (MMP-3)-mediated gene therapy for glaucoma.  |  O'Callaghan, J., et al. 2023. Sci Adv. 9: eadf6537. PMID: 37075118
  5. MMP-3-mediated cleavage of OPN is involved in copper oxide nanoparticle-induced activation of fibroblasts.  |  Zhang, Y., et al. 2023. Part Fibre Toxicol. 20: 22. PMID: 37217992
  6. MMP-3基因敲除可诱导整体转录变化，并减少缺血性中风模型中男性和女性的脑梗死。  |  Hamblin, MH., et al. 2024. Int J Mol Sci. 25: PMID: 39000490
  7. MMP-3 mediates copper oxide nanoparticle-induced pulmonary inflammation and fibrosis.  |  Zhang, Y., et al. 2024. J Nanobiotechnology. 22: 428. PMID: 39030581
  8. N末端在胶原酶 '超活化 '过程中作用的结构含义。晶体学研究。  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
  9. 基质金属蛋白酶：综述。  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
  10. 软骨素 2 对人类中性粒细胞胶原酶的活化作用  |  Knäuper, V., et al. 1996. Eur J Biochem. 235: 187-91. PMID: 8631328
  11. 人类单核细胞中几种基质金属蛋白酶基因的表达。  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704
  12. cDNA 克隆和小鼠 Stromelysin-2 (MMP-10) 基因的表达。  |  Madlener, M. and Werner, S. 1997. Gene. 202: 75-81. PMID: 9427548

订购信息

产品名称产品编号规格价格数量收藏夹

MMP-3 抗体 (1B4)

sc-21732
200 µg/ml
$316.00

MMP-3 (1B4): m-IgG Fc BP-HRP 套装

sc-526602
200 µg Ab; 10 µg BP
$354.00

MMP-3 (1B4): m-IgG1 BP-HRP 套装

sc-531975
200 µg Ab; 20 µg BP
$354.00

MMP-3 抗体 (1B4) AC

sc-21732 AC
500 µg/ml, 25% agarose
$416.00

MMP-3 抗体 (1B4) HRP

sc-21732 HRP
200 µg/ml
$316.00

MMP-3 抗体 (1B4) FITC

sc-21732 FITC
200 µg/ml
$330.00

MMP-3 抗体 (1B4) PE

sc-21732 PE
200 µg/ml
$343.00

MMP-3 抗体 (1B4) Alexa Fluor® 488

sc-21732 AF488
200 µg/ml
$357.00

MMP-3 抗体 (1B4) Alexa Fluor® 546

sc-21732 AF546
200 µg/ml
$357.00

MMP-3 抗体 (1B4) Alexa Fluor® 594

sc-21732 AF594
200 µg/ml
$357.00

MMP-3 抗体 (1B4) Alexa Fluor® 647

sc-21732 AF647
200 µg/ml
$357.00

MMP-3 抗体 (1B4) Alexa Fluor® 680

sc-21732 AF680
200 µg/ml
$357.00

MMP-3 抗体 (1B4) Alexa Fluor® 790

sc-21732 AF790
200 µg/ml
$357.00

i am from brazil and i wanna buythis antibody. do you have any dealer here in Brazil?

Asked by: thiago35
Thank you for your question. You can find our local distributor under this link by selecting your country from the list: https://www.scbt.com/resources/distributors
Answered by: Technical Support Europe
Date published: 2020-10-16

I am looking for a good MMP-3 antibody for rat brain lysate. The antibody I have used in the past from RDI is no longer available. Could I please receive the 10ul free sample so I can try this one out?

Asked by: Mike26
Thank you for your question. Please contact Technical Service for more information on how to receive a 10 μg sample of our well characterized monoclonal antibody.
Answered by: Technical Support
Date published: 2018-12-05

Can you recommend a positive control to use with MMP-3 Antibody (1B4): sc-21732 for Western blot?

Asked by: jenni
We recommend HT-1080 (sc-364183), ECV 304 (sc-2269), HUV-EC-C (sc-364180), WI 38 (sc-364260) or CCD-1064SK (sc-2263) whole cell lysates to use as positive controls as we have Western blot data showing specific detection of MMP-3 in these cell lines.
Answered by: Technical Support 6
Date published: 2017-01-09
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Rated 5 out of 5 by from Works great for WBWorks well on neonatal mouse lungs at concentration of 30ug. Antibody dilution will be 1:200 for overnight at 4oC
Date published: 2024-12-13
Rated 1 out of 5 by from Test for WBThe antibody does not work fine in our hand for WB on MCF-7, HT29, HCT116 and SHSY5Y cells.
Date published: 2023-11-10
Rated 5 out of 5 by from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 5 out of 5 by from UPENNGα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 5 out of 5 by from work well一个月前我申请了该产品的试用装，最近用该抗体跑出了明显条带，抗体可靠实用灵敏，品质不错，值得再次购买
Date published: 2017-10-19
Rated 4 out of 5 by from Work well in WB analysisThis antibody works well in western blot analysis of mouse liver lysate
Date published: 2017-10-02
Rated 5 out of 5 by from good WB dataOur lab had great WB results with the MMP-3 (1B4) antibody for mouse brain lysates. 1:250
Date published: 2017-09-26
Rated 3 out of 5 by from No band detected in U87 cell lysates (WB)No band detected in U87 cell lysates via western blot analysis (1:1000)
Date published: 2017-09-10
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MMP-3 抗体 (1B4) is rated 4.4 out of 5 by 20.
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