Date published: 2025-9-7
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MMP2 Antibody (8B4) - Western Blotting - Image 403740
MMP2 抗体 (8B4): sc-13595. 人重组 MMP-2 融合蛋白的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 使用的检测试剂: m-IgG1 BP-CFL 790: sc-533666.
MMP2 抗体 (8B4): sc-13595. 人重组 MMP-2 融合蛋白的近红外 Western 印迹分析. 使用 UltraCruz® 阻断试剂: sc-516214. 使用的检测试剂: m-IgG1 BP-CFL 790: sc-533666.

MMP2 抗体 (8B4): sc-13595

4.5(23)
寫評論提問

说明书
  • MMP2抗体(8B4)是小鼠单克隆IgG1 κ, 在240篇文献中引用，规格为200 µg/ml
  • 针对human来源的激活重组MMP-2
  • 抗-MMP2 抗体 (8B4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 MMP-2
  • 抗MMP2抗体（8B4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MMP2 (8B4): sc-13595的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 MMP2 抗体 (8B4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MMP2 抗体 (8B4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MMP 抗体 包括 MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-2, MMP-21, MMP-26, MMP-3和MMP-7

MMP-2抗体（8B4）是一种小鼠单克隆IgG1 kappa轻链抗体，用于检测活化的人MMP-2以及大鼠和小鼠来源的MMP-2，可用于包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）在内的各种应用。MMP-2单克隆抗体（8B4）针对活化的MMP-2产生，可确保精确结合，有非结合型和结合型两种形式，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）以及多种Alexa Fluor®结合物，可满足不同的实验需求。基质金属蛋白酶-2（MMP-2）也称为明胶酶A、CLG4、MONA、CLG4A和TBE-1，是基质金属蛋白酶家族中的一种关键酶，主要存在于细胞外基质（ECM ），MMP-2在降解ECM成分（包括IV、V、VII和X型胶原蛋白以及I型明胶）中起着至关重要的作用。MMP-2的细胞外定位通过促进ECM屏障的破坏和细胞迁移，实现组织重塑、血管生成和伤口愈合。这种定位在癌症转移中变得至关重要，MMP-2介导的ECM降解使癌细胞得以入侵和扩散。MMP-2还可以调节生物活性ECM分子，影响细胞信号通路，从而控制细胞增殖、分化及凋亡。Ras信号通路调节MMP-2的活化和分泌，而活化和分泌需要钙离子和锌离子来催化。了解MMP-2的定位和调节对于研究心血管疾病、自身免疫性疾病和炎症的机理仍然至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MMP2 抗体 (8B4)

sc-13595
200 µg/ml
$316.00

MMP2 (8B4): m-IgG Fc BP-HRP 套装

sc-528290
200 µg Ab; 10 µg BP
$354.00

MMP2 (8B4): m-IgGκ BP-HRP 套装

sc-520631
200 µg Ab, 40 µg BP
$354.00

MMP2 抗体 (8B4) AC

sc-13595 AC
500 µg/ml, 25% agarose
$416.00

MMP2 抗体 (8B4) HRP

sc-13595 HRP
200 µg/ml
$316.00

MMP2 抗体 (8B4) FITC

sc-13595 FITC
200 µg/ml
$330.00

MMP2 抗体 (8B4) PE

sc-13595 PE
200 µg/ml
$343.00

MMP2 抗体 (8B4) Alexa Fluor® 488

sc-13595 AF488
200 µg/ml
$357.00

MMP2 抗体 (8B4) Alexa Fluor® 546

sc-13595 AF546
200 µg/ml
$357.00

MMP2 抗体 (8B4) Alexa Fluor® 594

sc-13595 AF594
200 µg/ml
$357.00

MMP2 抗体 (8B4) Alexa Fluor® 647

sc-13595 AF647
200 µg/ml
$357.00

MMP2 抗体 (8B4) Alexa Fluor® 680

sc-13595 AF680
200 µg/ml
$357.00

MMP2 抗体 (8B4) Alexa Fluor® 790

sc-13595 AF790
200 µg/ml
$357.00

What to use for positive control in Western blotting?

Asked by: DrMORO617
Thank you for your question. Below are some suggested products. https://www.scbt.com/p/ecv304-cell-lysate?requestFrom=search https://www.scbt.com/p/a-375-cell-lysate?requestFrom=search
Answered by: BlakeJ
Date published: 2023-09-19

what tissue i can use for positive control in immunohistochemistry study?

Asked by: nevi
Thank you for your question. MMP-2 has high expression in placenta, epididymis and gall bladder.
Answered by: Technical Support
Date published: 2019-06-16

can i use this antibody for angiogenesis study- MMP-2 Antibody (8B4): sc-13595

Asked by: sambhavi
Thank you for your question. We have not used this antibody for that research application, and can not find any reference to support it.
Answered by: Technical Support
Date published: 2019-05-25

I am trying to detect MMP-2 in my zebrafish samples. Will this antibody work?

Asked by: Cweed
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: TechService7
Date published: 2016-12-21

I have run out of my goat anti-mouse secondary antibody and it is no longer available. What do you suggest I use for detection?

Asked by: Cweed
We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: TechService7
Date published: 2016-12-21
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Rated 4 out of 5 by from Great for WBIt is a good antibody for western blot. I'd recommend it.
Date published: 2024-04-01
Rated 1 out of 5 by from this antibody is not workingthis antibody is not working for western blot at 1:1000 and 1:500
Date published: 2023-07-02
Rated 4 out of 5 by from This antibody works greatI have used at 1:1000 dilution and the bands were beautiful
Date published: 2022-04-07
Rated 5 out of 5 by from Great antibody for WBThis primary antibody worked very well in Western blot.
Date published: 2021-03-31
Rated 5 out of 5 by from 美しいバンドが検出されます。WBで使用しました。シャープなバンドが検出されました。非特異的なバンドは一切ありません。バンドの大きさもデータシートと同じ位置です。
Date published: 2020-12-01
Rated 3 out of 5 by from 見えませんでした。サンプルでいただきました。癌細胞を使ってウエスタンブロットで確認。濃度は濃く使用しましたが、検出されませんでした。
Date published: 2020-08-19
Rated 5 out of 5 by from Great for immunofluorescenceI purchased this Ab about a month ago and have had great results in my immunofluorescence staining.
Date published: 2019-11-06
Rated 5 out of 5 by from Works good for WBIt's a great product for me to analyze Western blotting.
Date published: 2019-03-19
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MMP2 抗体 (8B4) is rated 4.5 out of 5 by 23.
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