MMP-2抗体 (2C1) 推荐用于 mouse, rat 和 human 来源的MMP-2 WB, IP 和 IF检测

MMP-2 抗体 (2C1): sc-13594

MMP-2抗体 (2C1) is rated 4.3 out of 5 by 41.
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说明书

    • 抗-MMP-2 抗体 2C1 是小鼠单克隆 IgG1 (kappa轻链),MMP-2抗体,规格为200 µg/ml
    • 免疫自human来源的激活形式的MMP-2
    • 抗-MMP-2 抗体 (2C1) 推荐用于 WB, IP 和 IF,检测mouse, rat 和human 来源的 MMP-2
    • MMP-2 抗体 (2C1) 可偶联 琼脂糖 用于 IP; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取MMP-2 (2C1): sc-13594免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Does it work for bovine tissue?

Asked by: Anonymous
Thank you for your question. This antibody has not yet been tested in bovine samples and so that is not a recommended species at this time. We are still gathering data to show on our website. We do have a previous citation in bovine samples which may be helpful for you: PMID: 12844104.
Answered by: Tech Service
Date published: 2019-11-20

We are looking for a MMP-2 antibody that works well in Western blotting . For this we would like to use a 10ug free sample of MMP2 antibody (2C1): sc-13594. I was wondering if we could get some.

Asked by: Shiv
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-02-26

Hello.. is there a recommended buffer for use of this product with tissue lysates and immunoblotting? We usually use RIPA buffer for protein expression, but was concerned about chelators.

Asked by: Nabilb
Thank you for your question. We do not have any specific buffer recommendation for use with this MMP-2 antibody and have prepared our lysates for testing using our standard RIPA buffer. You may want to refer to some of the numerous product citations using this antibody for Western blotting to see if they have any optimized recommendations.
Answered by: Technical Support
Date published: 2019-01-26

What is the epitope sequence (binding site) of this antibody? Could you tell me a.a. number?

Asked by: neoSong
Thank you for your question. This mouse monoclonal antibody, MMP-2 (2C1): sc-13594, is raised against the activated form of MMP-2 of human origin. The exact epitope for this monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2017-09-14

Can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anna99
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This monoclonal for MMP-2 is available conjugated to agarose (sc-13594 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-13594 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-13594 PE), fluorescein (sc-13594 FITC), Alexa Fluor® 488 (sc-13594 AF488) or Alexa Fluor® 647 (sc-13594 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 5 out of 5 by from UPENN Gα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 3 out of 5 by from 抗体特异性还可以 用着效果还行。带的大小有些不确定,最上一条带在70kd以下,认为是剪切的mmp2,所以下面的条带不确定是什么。
Date published: 2018-03-20
Rated 5 out of 5 by from Great product MMP-2 antibody works well at the concentration of 1:500 with satisfied bands
Date published: 2018-02-08
Rated 5 out of 5 by from western blot This antibody generates a clearsignal with the HepG2 cell lysis (1:200).
Date published: 2018-01-25
Rated 1 out of 5 by from Unsucessful western blot with glioblastoma cells I have used this antibody 1:100-1:200 for western blotting and failled to obtain any band with the expected molecular weight using lysates from human glioblastoma cell lines
Date published: 2017-11-28
Rated 2 out of 5 by from May not be suitable for IF Sample:muscle cryosection Applications: IF 1:100, 1:200, 1:400 Results: Very weak staining. A little bit staining in nuclear. No difference with negetive control.
Date published: 2017-11-22
Rated 1 out of 5 by from Didnot work for WB did not work for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from work well 一月前我向贵公司申请了该抗体的试用装,结果非常好,条带清楚明显,位置正确,抗体很不错,可靠有用,值得再次购买
Date published: 2017-10-19
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