Date published: 2025-9-7
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MMP2 Antibody (2C1) - Western Blotting - Image 109916
MMP-2 抗体 (2C1): sc-13594. 未转染 293: sc-110760 (A), 转染人 MMP-2 的 293: sc-176407 (B) 和 A-375 (C) 全细胞裂解液中 MMP-2 表达的 Western 印迹分析.
MMP2 Antibody (2C1) - Western Blotting - Image 109916
MMP-2 抗体 (2C1): sc-13594. 未转染 293: sc-110760 (A), 转染人 MMP-2 的 293: sc-176407 (B) 和 A-375 (C) 全细胞裂解液中 MMP-2 表达的 Western 印迹分析.
MMP2 Antibody (2C1) - Western Blotting - Image 3842
MMP-2 抗体 (2C1): sc-13594. 人重组 MMP-2 的 Western 印迹分析.
MMP-2 抗体 (2C1): sc-13594. 未转染 293: sc-110760 (A), 转染人 MMP-2 的 293: sc-176407 (B) 和 A-375 (C) 全细胞裂解液中 MMP-2 表达的 Western 印迹分析.

MMP2 抗体 (2C1): sc-13594

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寫評論提問

说明书
  • MMP2 抗体 2C1 是小鼠单克隆 IgG1 κ，MMP2抗体, 在178篇文献中引用，规格为200 µg/ml
  • 针对MMP-2的激活形式（human起源）
  • MMP2 抗体 (2C1) 推荐用于 WB, IP 和 IF，检测mouse, rat 和human 来源的 MMP-2
  • MMP2抗体（2C1）可与琼脂糖结合用于IP，与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MMP2 (2C1): sc-13594的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是MMP2 Antibody (2C1) for WB applications. 的首选辅助检测试剂，这些试剂现与MMP2 Antibody (2C1) 打包提供（请参阅下面的订购信息）。

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相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

额外MMP抗体，包括MMP-1, MMP-10, MMP-11, MMP-12, MMP-13, MMP-2, MMP-21, MMP-26, MMP-3, MMP-7, MMP-8和MMP-9

MMP-2抗体（2C1）是一种小鼠单克隆IgG1 kappa轻链抗体，专门用于检测人类MMP-2的激活形式，以及小鼠和大鼠来源的MMP-2，可用于多种应用，包括蛋白质印迹（WB）、免疫沉淀（IP）和免疫荧光（IF）。MMP-2单克隆抗体（2C1）针对活化的MMP-2，可确保与目标蛋白精确结合，有非结合型和多种结合型可供选择，如琼脂糖、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物，以满足不同的实验需求。基质金属蛋白酶-2，也称为MMP-2、明胶酶A、CLG4、MONA、CLG4A和TBE-1，是基质金属蛋白酶（MMP）家族中的一种关键酶，在降解细胞外 基质（ECM）成分，包括IV、V、VII和X型胶原蛋白以及I型明胶。这种酶活性对于正常的生理过程（如组织重塑、血管生成和伤口愈合）以及病理状况（如癌症转移、心血管疾病和炎症性疾病）至关重要。MMP-2的活性受Ras信号传导途径的严格调控，需要钙离子和锌离子才能发挥催化功能。MMP-2通过切割ECM蛋白，促进细胞迁移和侵袭，是肿瘤进展和转移的关键因素。此外，MMP-2还参与调节ECM中的生物活性分子，影响细胞信号传导通路，从而控制细胞的增殖、分化及凋亡。了解MMP-2的表达和调节对于阐明各种疾病的潜在机制至关重要，而抗MMP-2抗体（2C1）是研究人员研究这些关键生物学过程的宝贵工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MMP2 抗体 (2C1) 参考文献:

  1. Src 转化的 3Y1 需要 Ras 通路来激活 MMP-2 的分泌和入侵。  |  Thant, AA., et al. 1999. Oncogene. 18: 6555-63. PMID: 10597259
  2. 完成人类 IV 型胶原酶前酶的一级结构，并将基因（CLG4）分配到 16 号染色体的 q21 区域。  |  Huhtala, P., et al. 1990. Genomics. 6: 554-9. PMID: 2158484
  3. 人类 IV 型胶原酶基因的结构。  |  Huhtala, P., et al. 1990. J Biol Chem. 265: 11077-82. PMID: 2162831
  4. H-ras 癌基因转化的人类支气管上皮细胞（TBE-1）会分泌一种能够降解基底膜胶原蛋白的单一金属蛋白酶。  |  Collier, IE., et al. 1988. J Biol Chem. 263: 6579-87. PMID: 2834383
  5. N末端在胶原酶 '超活化 '过程中作用的结构含义。晶体学研究。  |  Reinemer, P., et al. 1994. FEBS Lett. 338: 227-33. PMID: 8307185
  6. 基质金属蛋白酶：综述。  |  Birkedal-Hansen, H., et al. 1993. Crit Rev Oral Biol Med. 4: 197-250. PMID: 8435466
  7. 人类单核细胞中几种基质金属蛋白酶基因的表达。  |  Machein, U. and Conca, W. 1997. Adv Exp Med Biol. 421: 247-51. PMID: 9330704

订购信息

产品名称产品编号规格价格数量收藏夹

MMP2 抗体 (2C1)

sc-13594
200 µg/ml
$316.00

MMP2 (2C1): m-IgG Fc BP-HRP 套装

sc-526566
200 µg Ab; 10 µg BP
$354.00

MMP2 (2C1): m-IgG1 BP-HRP 套装

sc-531939
200 µg Ab; 20 µg BP
$354.00

MMP2 抗体 (2C1) AC

sc-13594 AC
500 µg/ml, 25% agarose
$416.00

MMP2 抗体 (2C1) FITC

sc-13594 FITC
200 µg/ml
$330.00

MMP2 抗体 (2C1) PE

sc-13594 PE
200 µg/ml
$343.00

MMP2 抗体 (2C1) Alexa Fluor® 488

sc-13594 AF488
200 µg/ml
$357.00

MMP2 抗体 (2C1) Alexa Fluor® 546

sc-13594 AF546
200 µg/ml
$357.00

MMP2 抗体 (2C1) Alexa Fluor® 594

sc-13594 AF594
200 µg/ml
$357.00

MMP2 抗体 (2C1) Alexa Fluor® 647

sc-13594 AF647
200 µg/ml
$357.00

MMP2 抗体 (2C1) Alexa Fluor® 680

sc-13594 AF680
200 µg/ml
$357.00

MMP2 抗体 (2C1) Alexa Fluor® 790

sc-13594 AF790
200 µg/ml
$357.00

Does it work for bovine tissue?

Asked by: Anonymous
Thank you for your question. This antibody has not yet been tested in bovine samples and so that is not a recommended species at this time. We are still gathering data to show on our website. We do have a previous citation in bovine samples which may be helpful for you: PMID: 12844104.
Answered by: Tech Service
Date published: 2019-11-20

We are looking for a MMP-2 antibody that works well in Western blotting . For this we would like to use a 10ug free sample of MMP2 antibody (2C1): sc-13594. I was wondering if we could get some.

Asked by: Shiv
Please contact our Technical Service Department for more information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Technical Support
Date published: 2019-02-26

Hello.. is there a recommended buffer for use of this product with tissue lysates and immunoblotting? We usually use RIPA buffer for protein expression, but was concerned about chelators.

Asked by: Nabilb
Thank you for your question. We do not have any specific buffer recommendation for use with this MMP-2 antibody and have prepared our lysates for testing using our standard RIPA buffer. You may want to refer to some of the numerous product citations using this antibody for Western blotting to see if they have any optimized recommendations.
Answered by: Technical Support
Date published: 2019-01-26

What is the epitope sequence (binding site) of this antibody? Could you tell me a.a. number?

Asked by: neoSong
Thank you for your question. This mouse monoclonal antibody, MMP-2 (2C1): sc-13594, is raised against the activated form of MMP-2 of human origin. The exact epitope for this monoclonal antibody has not yet been determined.
Answered by: Technical Support
Date published: 2017-09-14

Can this mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: Anna99
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This monoclonal for MMP-2 is available conjugated to agarose (sc-13594 AC), 500 µg/0.25 ml agarose in 1 ml, for IP; HRP (sc-13594 HRP), 200 µg/ml, for WB, IHC(P) and ELISA; phycoerythrin (sc-13594 PE), fluorescein (sc-13594 FITC), Alexa Fluor® 488 (sc-13594 AF488) or Alexa Fluor® 647 (sc-13594 AF647), 200 µg/ml, for IF, IHC(P) and FCM
Answered by: Technical Support 13
Date published: 2017-01-04
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Rated 2 out of 5 by from Faint staining in primary human fibroblastsShowed a very faint band in WB analysis using primary human fibroblasts.
Date published: 2024-08-23
Rated 5 out of 5 by from 与其他品牌相比，Santa产品更易显现阳性结果。我之前购买过该类产品，Stat3,JAK2等，用于胃癌肺转移动物模型IHC及WB检测，实验结果很满意，检测灵敏度高。
Date published: 2024-01-09
Rated 5 out of 5 by from UPENNGα 12 Antibody is very good. The band is very stronger.
Date published: 2018-04-19
Rated 3 out of 5 by from 抗体特异性还可以用着效果还行。带的大小有些不确定，最上一条带在70kd以下，认为是剪切的mmp2，所以下面的条带不确定是什么。
Date published: 2018-03-20
Rated 5 out of 5 by from Great productMMP-2 antibody works well at the concentration of 1:500 with satisfied bands
Date published: 2018-02-08
Rated 5 out of 5 by from western blotThis antibody generates a clearsignal with the HepG2 cell lysis (1:200).
Date published: 2018-01-25
Rated 1 out of 5 by from Unsucessful western blot with glioblastoma cellsI have used this antibody 1:100-1:200 for western blotting and failled to obtain any band with the expected molecular weight using lysates from human glioblastoma cell lines
Date published: 2017-11-28
Rated 2 out of 5 by from May not be suitable for IFSample:muscle cryosection Applications: IF 1:100, 1:200, 1:400 Results: Very weak staining. A little bit staining in nuclear. No difference with negetive control.
Date published: 2017-11-22
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MMP2 抗体 (2C1) is rated 4.2 out of 5 by 43.
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