Date published: 2025-9-8

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MMP2 抗体 (4D3): sc-53630

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说明书
  • MMP2抗体(4D3)是小鼠单克隆IgG1 κ, 在88篇文献中引用,规格为200 µg/ml
  • 免疫human物种的MMP2的氨基酸557-569
  • 抗-MMP2 抗体 (4D3) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 MMP-2; 也和以下物种反应,包括: rabbit; 可能与 MMP-3产生交联反应
  • 抗MMP2抗体(4D3)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MMP2 (4D3): sc-53630免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 是 MMP2 抗体 (4D3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与MMP2 抗体 (4D3) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

MMP-2 抗体(4D3)是一种小鼠单克隆 IgG1 kappa 轻链抗体,能识别并结合人基质金属蛋白酶-2(MMP-2)蛋白的 557-569 氨基酸残基,同时还能与小鼠、大鼠和其他物种(包括兔子)的 MMP-2 发生反应,并可能与 MMP-3 发生交叉反应。MMP-2 单克隆抗体 (4D3) 建议用于各种应用,如西部印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和石蜡包埋切片免疫组化(IHCP)。MMP-2又称明胶酶A、CLG4、MONA、CLG4A和TBE-1,是基质金属蛋白酶(MMP)家族中的一种重要酶、 一个催化结构域,其中含有对其蛋白水解活性至关重要的锌结合基序,以及一个类似血卟啉的结构域,该结构域具有底物特异性,并介导与金属蛋白酶组织抑制剂(TIMPs)的相互作用。MMP-2 的结构完整性对其降解细胞外基质(ECM)成分(如胶原 IV 型、V 型、VII 型和 X 型以及明胶 I 型)、促进组织重塑、血管生成和伤口愈合等过程至关重要。要激活 MMP-2,丙肽结构域必须被裂解,这一过程受 Ras 信号通路调控,并依赖于钙离子和锌离子的存在来发挥催化活性。血卟啉样结构域不仅决定了底物的特异性,而且在该酶与抑制剂的相互作用中起着关键作用,从而调节在细胞环境中的活性。抗 MMP-2 抗体(克隆 4D3)推荐用于研究 MMP-2 在细胞外基质重塑、血管生成和伤口愈合中的作用,以及基质金属蛋白酶家族和抑制剂的调节和相互作用。MMP-2 (4D3) 抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物,可满足不同的实验要求。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

MMP2 抗体 (4D3)

sc-53630
200 µg/ml
$316.00

MMP2 (4D3): m-IgG Fc BP-HRP 套装

sc-525614
200 µg Ab; 10 µg BP
$354.00

MMP2 抗体 (4D3) AC

sc-53630 AC
500 µg/ml, 25% agarose
$416.00

MMP2 抗体 (4D3) HRP

sc-53630 HRP
200 µg/ml
$316.00

MMP2 抗体 (4D3) FITC

sc-53630 FITC
200 µg/ml
$330.00

MMP2 抗体 (4D3) PE

sc-53630 PE
200 µg/ml
$343.00

MMP2 抗体 (4D3) Alexa Fluor® 488

sc-53630 AF488
200 µg/ml
$357.00

MMP2 抗体 (4D3) Alexa Fluor® 546

sc-53630 AF546
200 µg/ml
$357.00

MMP2 抗体 (4D3) Alexa Fluor® 594

sc-53630 AF594
200 µg/ml
$357.00

MMP2 抗体 (4D3) Alexa Fluor® 647

sc-53630 AF647
200 µg/ml
$357.00

MMP2 抗体 (4D3) Alexa Fluor® 680

sc-53630 AF680
200 µg/ml
$357.00

MMP2 抗体 (4D3) Alexa Fluor® 790

sc-53630 AF790
200 µg/ml
$357.00

Does this antibody work in western blots of mouse cells? Your illustrations seem to be under conditions of overexpression

Asked by: Brian2
Thank you for your question. MMP-2 (4D3) is recommended for mouse, rat, and human samples .
Answered by: BlakeJ
Date published: 2023-06-07

Can sc-53630: MMP-2 (4D3) be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: jenniferc
Thank you for your inquiry. Yes, sc-53630: MMP-2 (4D3) is validated for use in IHC with formalin fixed, paraffin-embedded tissue sections. We recommend performing antigen retrieval with sodium citrate buffer, pH 6, and heat. The complete protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 10
Date published: 2017-01-10
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Rated 4 out of 5 by from Work well in WB analysisThis antibody works well in western blot analysis of mouse liver lysate
Date published: 2017-10-02
Rated 5 out of 5 by from Very clear bandI got the very clear band and I like this antibody.
Date published: 2017-08-31
Rated 5 out of 5 by from stong band present in western blot analysisI used this antibody for WB, this product use effect is very good
Date published: 2017-05-19
Rated 5 out of 5 by from Great WB and IHCOutstanding product for western blot and IHC staining; beats polyclonal alternatives
Date published: 2017-01-12
Rated 1 out of 5 by from Hope to find a working MMP2 antibodyI bough this antibody but it did not Work with immunohistochemistry from paraffin embedded heart samples
Date published: 2016-12-27
Rated 5 out of 5 by from Works well for IHCShows strong nuclear and cytoplasmic staining in immunohistochemistry with paraffin-embedded tissue samples!
Date published: 2016-12-16
Rated 3 out of 5 by from Reasonably good banding observed in WesternReasonably good banding observed in Western Blot with recombinant protein and whole cell lysates -SCBT QC
Date published: 2015-03-22
Rated 5 out of 5 by from Works very well in IHC(P) with human lymph nodesWorks very well in IHC(P) with human lymph nodes -SCBT Publication Review
Date published: 2015-03-02
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MMP2 抗体 (4D3) is rated 4.1 out of 5 by 8.
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