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Mfn2/Mitofusin 2 Antibody (F-5) - Western Blotting - Image 378720
Mfn2 抗体 (F-5): sc-515647. Hep G2 (A), RD (B), A-673 (C), c4 (D), WEHI-231 (E) 和 TK-1 (F) 全细胞裂解液中 Mfn2 表达的 Western 印迹分析.
Mfn2 抗体 (F-5): sc-515647. Hep G2 (A), RD (B), A-673 (C), c4 (D), WEHI-231 (E) 和 TK-1 (F) 全细胞裂解液中 Mfn2 表达的 Western 印迹分析.

Mfn2/Mitofusin 2 抗体 (F-5): sc-515647

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寫評論提問

说明书
  • Mfn2/Mitofusin 2 抗体 F-5 是小鼠单克隆 IgG1 κ，Mfn2/Mitofusin 2抗体, 在53篇文献中引用，规格为200 µg/ml
  • 针对氨基酸461-528，在human起源的Mfn2的细胞质域内进行映射
  • Mfn2/Mitofusin 2 抗体 (F-5) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 Mfn2
  • 抗Mfn2/Mitofusin 2抗体（F-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Mfn2/Mitofusin 2 (F-5): sc-515647的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG1 BP-HRP和m-IgGκ BP-HRP是Mfn2/Mitofusin 2 Antibody (F-5) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Mfn2/Mitofusin 2 Antibody (F-5) 打包提供（请参阅下面的订购信息）。

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描述
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说明书与实验方案
研究信息

関連項目

额外Mfn抗体，包括Mfn2

Mfn2抗体（F-5）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的Mfn2蛋白。Mfn2 (F-5) 抗体有非结合型和多种结合型可供选择，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。Mfn2蛋白（又称线粒体融合蛋白2）通过调节线粒体融合在维持线粒体功能和细胞能量平衡方面发挥着关键作用。这一过程在心脏和肌肉等能量需求高的组织中尤为重要，因为Mfn2在这些组织中大量表达。Mfn2的正常运作对于胚胎发育至关重要，并涉及包括肥胖症在内的多种代谢紊乱的病理生理学。值得注意的是，Mfn2与线粒体动力学中其他蛋白（如Mfn1）相互作用，这些相互作用对于形成支持细胞代谢的线粒体网络至关重要。了解Mfn2的作用及其相互作用有助于深入了解线粒体相关疾病和潜在的治疗靶点。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Mfn2/Mitofusin 2 抗体 (F-5) 参考文献:

  1. 通过调节Mfn2靶向线粒体动力学，治疗糖尿病性心肌病。  |  Hu, L., et al. 2019. Theranostics. 9: 3687-3706. PMID: 31281507
  2. Mfn2通过PERK通路调控高血糖诱导的荚膜细胞MAMs功能障碍和凋亡  |  Cao, Y., et al. 2021. Front Cell Dev Biol. 9: 769213. PMID: 34988075
  3. Mfn2介导的线粒体融合可缓解多柔比星诱导的心脏毒性，并通过代谢转换增强其抗癌活性。  |  Ding, M., et al. 2022. Redox Biol. 52: 102311. PMID: 35413642
  4. Paeonol通过激活PKCε-Stat3通路促进Mfn2介导的线粒体融合，从而防止阿霉素诱导的心脏毒性。  |  Ding, M., et al. 2023. J Adv Res. 47: 151-162. PMID: 35842187
  5. Mfn2介导的线粒体融合通过AMPK/mTOR/ERK信号通路减少ROS，从而促进自噬并抑制卵巢癌的进展。  |  Ashraf, R. and Kumar, S. 2022. Cell Mol Life Sci. 79: 573. PMID: 36308626
  6. 通过调节Mfn2介导的线粒体自噬，Morinda officinalis寡糖可减轻高血压大鼠的抑郁样行为。  |  Yang, L., et al. 2023. J Neuroinflammation. 20: 31. PMID: 36765376
  7. MFN2通过调节线粒体依赖性EGFR的去磷酸化，抑制透明细胞肾细胞癌的进展。  |  Luo, L., et al. 2023. Cancer Commun (Lond). 43: 808-833. PMID: 37378422
  8. PGAM5是一种MFN2磷酸酶，在调控线粒体动力学方面发挥着重要作用。  |  Nag, S., et al. 2023. Cell Rep. 42: 112895. PMID: 37498743
  9. LRRK2通过促进MFN2降解和线粒体完整性异常加重肾脏损伤。  |  Zhang, S., et al. 2023. Redox Biol. 66: 102860. PMID: 37633049
  10. Mfn2/Hsc70复合物介导线粒体-脂滴膜接触的形成并调节心肌脂质代谢。  |  Hu, L., et al. 2024. Adv Sci (Weinh). 11: e2307749. PMID: 38311582

订购信息

产品名称产品编号规格价格数量收藏夹

Mfn2/Mitofusin 2 抗体 (F-5)

sc-515647
200 µg/ml
$316.00

Mfn2/Mitofusin 2 (F-5): m-IgG Fc BP-HRP 套装

sc-531454
200 µg Ab; 10 µg BP
$354.00

Mfn2/Mitofusin 2 (F-5): m-IgGκ BP-HRP 套装

sc-525146
200 µg Ab, 40 µg BP
$354.00

Mfn2/Mitofusin 2 (F-5): m-IgG1 BP-HRP 套装

sc-544566
200 µg Ab; 20 µg BP
$354.00

Mfn2/Mitofusin 2 抗体 (F-5) AC

sc-515647 AC
500 µg/ml, 25% agarose
$416.00

Mfn2/Mitofusin 2 抗体 (F-5) HRP

sc-515647 HRP
200 µg/ml
$316.00

Mfn2/Mitofusin 2 抗体 (F-5) FITC

sc-515647 FITC
200 µg/ml
$330.00

Mfn2/Mitofusin 2 抗体 (F-5) PE

sc-515647 PE
200 µg/ml
$343.00

Mfn2/Mitofusin 2 抗体 (F-5) Alexa Fluor® 488

sc-515647 AF488
200 µg/ml
$357.00

Mfn2/Mitofusin 2 抗体 (F-5) Alexa Fluor® 546

sc-515647 AF546
200 µg/ml
$357.00

Mfn2/Mitofusin 2 抗体 (F-5) Alexa Fluor® 594

sc-515647 AF594
200 µg/ml
$357.00

Mfn2/Mitofusin 2 抗体 (F-5) Alexa Fluor® 647

sc-515647 AF647
200 µg/ml
$357.00

Mfn2/Mitofusin 2 抗体 (F-5) Alexa Fluor® 680

sc-515647 AF680
200 µg/ml
$357.00

Mfn2/Mitofusin 2 抗体 (F-5) Alexa Fluor® 790

sc-515647 AF790
200 µg/ml
$357.00

Where can I check the Immunogen information (Sequence) ?

Asked by: Alan Michael
Thank you for your question. Mfn2/Mitofusin 2 Antibody (F-5): sc-515647 was raised against amino acids 461-528 mapping within a cytoplasmic domain of Mfn2 of human origin.
Answered by: Tech Support Europe
Date published: 2024-04-09

which band is correct, 55-43 or 86?

Asked by: Anonymous
Thank you for your question. According to Uniprot, Mfn2 can be expressed as two isoforms with predicted molecular weights of ~86 kDa and 50 kDa. https://www.uniprot.org/uniprot/O95140#sequences
Answered by: Technical Support
Date published: 2019-04-24

I need to use an antibody to detect the protein in samples of porcine origin. Do you know if Mfn2 (F-5): sc-515647 mouse monoclonal antibody will work?

Asked by: Randy McDonald
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support
Date published: 2017-02-28
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Rated 5 out of 5 by from Mitofusin works very well in IFMitofusin2 Ab works very well in IF in human tissues
Date published: 2023-05-16
Rated 3 out of 5 by from We didn't get any bands.Our sample is from rat brain and the protein content is more than 50 micro-gram. But we didn't get any bands.
Date published: 2021-07-21
Rated 5 out of 5 by from recognizes two very clear bandsThe antibody detects two very clear bands (c.a.86 kDa and 50 kDa) in primary human skin fibroblasts
Date published: 2020-02-16
Rated 1 out of 5 by from faint signalOnly faint signal in mouse myoblasts. Not as good as original.
Date published: 2018-10-03
Rated 5 out of 5 by from It works well for meYou can see the antibody works really well at 1:500 dilution.
Date published: 2018-09-29
Rated 3 out of 5 by from Works well for WBUsed at a dilution of 1:500 for lung lysates loaded at 30ug/well
Date published: 2018-06-25
Rated 3 out of 5 by from High backgroundUsed for IF. Expected mitochondrial staining could be detected, but with high background
Date published: 2018-02-23
Rated 4 out of 5 by from Great featuresI bought it a month ago, it works well with a clear band, but the dilution should be over 1:500.
Date published: 2017-10-30
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Mfn2/Mitofusin 2 抗体 (F-5) is rated 4.3 out of 5 by 22.
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