MDM2 Antibody (HDM2-323): sc-56154

MDM2 Antibody (HDM2-323) is a mouse monoclonal IgG2a antibody raised against amino acids 389-402 of human MDM2, suitable for applications such as western blotting (WB) and immunoprecipitation (IP), and reactive with human, mouse, and rat samples. MDM2 (Murine Double Minute 2) is an oncoprotein that serves as a crucial negative regulator of the tumor suppressor p53, playing a vital role in cell cycle control and apoptosis. MDM2 functions as an E3 ubiquitin ligase, promoting the ubiquitination and subsequent proteasomal degradation of p53, thus controlling cellular levels and activity. Post-translational modifications of MDM2, including phosphorylation, ubiquitination, sumoylation, and acetylation, are essential for modulating stability, localization, and interaction with p53 and other regulatory proteins. For instance, phosphorylation of MDM2 by kinases such as ATM, Akt, or MAPK can enhance or inhibit ubiquitination of p53, affecting the cellular response to DNA damage and stress. Ubiquitination of MDM2 can lead to degradation, creating an autoregulatory feedback loop that fine-tunes p53 activity. These post-translational modifications regulate the MDM2-p53 pathway, ensuring proper cell cycle progression and apoptosis. Dysregulation of MDM2′s modifications can result in impaired p53 function, leading to uncontrolled cell proliferation and tumorigenesis. Understanding the role of MDM2′s post-translational modifications is critical for cancer research and the development of therapeutic strategies targeting the MDM2-p53 interaction. Anti-MDM2 antibody (HDM2-323) provides a valuable tool for researchers investigating these mechanisms and the impact of MDM2 regulation on cellular function.

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-MDM2 Antibody (HDM2-323) has 33 citations in a variety of scientific publications.

Primary antibodies like Anti-MDM2 Antibody (HDM2-323) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

MDM2 Antibody (HDM2-323) Specifications

Alternate Product Name: MDM2

Product Clone Name: HDM2-323

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a

Amino Acids: 389-402

Epitope Target: MDM2

Epitope Species: human

Product Reactivity/Detection: MDM2

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP

Vialing Information: Each vial contains 100 µg IgG_2a in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 33 citations in various scientific publications

Available Conjugates/Forms for MDM2 Antibody (HDM2-323): N/A

The preferred MDM2 antibody is MDM2 Antibody (SMP14): sc-965.