Date published: 2025-9-6
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MBNL2 Antibody (3B4) - Western Blotting - Image 284348
MBNL2 抗体 (3B4): sc-136167. Caki-1 (A), SK-N-SH (B), HeLa (C) 和 U-87 MG (D) 全细胞裂解液及小鼠脑组织提取物 (E) 中 MBNL2 表达的 Western 印迹分析.
MBNL2 抗体 (3B4): sc-136167. Caki-1 (A), SK-N-SH (B), HeLa (C) 和 U-87 MG (D) 全细胞裂解液及小鼠脑组织提取物 (E) 中 MBNL2 表达的 Western 印迹分析.

MBNL2 抗体 (3B4): sc-136167

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寫評論提問

说明书
  • MBNL2抗体(3B4)是小鼠单克隆IgG2b κ, 在27篇文献中引用，规格为200 µg/ml
  • 免疫human物种的重组MBNL2
  • 抗-MBNL2 抗体 (3B4) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 MBNL2
  • 抗MBNL2抗体（3B4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取MBNL2 (3B4): sc-136167的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgGκ BP-HRP 是 MBNL2 抗体 (3B4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与MBNL2 抗体 (3B4) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 MBNL 抗体 包括 MBNL1, MBNL2和MBNL3

MBNL2抗体（3B4）是一种IgG2b κ小鼠单克隆MBNL2抗体，可通过WB、IP、IF和IHC(P)检测小鼠、大鼠和人的MBNL2。MBNL2抗体（3B4）可作为非共轭抗MBNL2抗体形式，以及多种共轭形式的抗MBNL2抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。前mRNA剪接是基因表达转录后调控的关键步骤。有几种蛋白质复合物参与适当的mRNA剪接和转运。肌肉盲蛋白MBNL1、MBNL2和MBNL3通过与不同的内含子位点结合的CUG-BP和ETR-3样因子的拮抗作用，促进不同前mRNA上特定外显子的包含或排除。MBNL1和MBNL2在体内与扩展的CUG重复序列相关，并在DM1和DM2（肌强直性营养不良症1型和2型）的核焦点中定位，这表明突变RNA的核积聚在DM1中是致病的，因此暗示肌肉盲蛋白MBNL1和MBNL2都参与这两种疾病的发病机理。MBNL2是一种367个氨基酸的蛋白质，它参与以RNA依赖性蛋白质定位机制将整合素α3招募到粘着斑中。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

MBNL2 抗体 (3B4) 参考文献:

  1. 与肌营养不良症相关的（CUG）(n)扩增对人类肌盲蛋白的招募。  |  Miller, JW., et al. 2000. EMBO J. 19: 4439-48. PMID: 10970838
  2. 在肌营养不良症 1 型和 2 型中，Muscleblind 定位于异常 RNA 的核病灶。  |  Mankodi, A., et al. 2001. Hum Mol Genet. 10: 2165-70. PMID: 11590133
  3. 在 DM1 和 DM2 细胞中，MBNL, MBLL 和 MBXL 三种蛋白质在体内与扩增重复转录本的核病灶共定域。  |  Fardaei, M., et al. 2002. Hum Mol Genet. 11: 805-14. PMID: 11929853
  4. MBNL1 是 DM1 病灶形成和胰岛素受体剪接异常的主要决定因素。  |  Dansithong, W., et al. 2005. J Biol Chem. 280: 5773-80. PMID: 15546872
  5. [肌营养不良症的分子途径]。  |  Ishiura, S. 2005. Nihon Rinsho. 63: 515-21. PMID: 15773354
  6. 在心脏发育过程中，激活与抑制之间的动态平衡调控着前核糖核酸（pre-mRNA）的替代剪接。  |  Ladd, AN., et al. 2005. Dev Dyn. 233: 783-93. PMID: 15830352
  7. 肌营养不良症中肌盲与 RNA 病灶的共定位与替代剪接的误调控是可分离的。  |  Ho, TH., et al. 2005. J Cell Sci. 118: 2923-33. PMID: 15961406
  8. Muscleblind蛋白家族：一类新兴的发育程序化替代剪接调控因子。  |  Pascual, M., et al. 2006. Differentiation. 74: 65-80. PMID: 16533306
  9. 钙蛋白酶-2介导 MBNL2 降解和神经退行性病变中的发育 RNA 处理程序  |  Wang, LH., et al. 2022. J Neurosci. 42: 5102-5114. PMID: 35606145
  10. 研究骨骼肌再生过程中 MBNL1 和 MBNL2 缺失的影响。  |  Yadava, RS., et al. 2024. Int J Mol Sci. 25: PMID: 38473933
  11. MBNL2 通过抑制 Krüppel-like factor4 的 SUMOylation 促进与衰老相关的心脏纤维化。  |  Lu, J., et al. 2024. iScience. 27: 110163. PMID: 38974966
  12. 预测人类不明基因的编码序列。VIII.78 个新的大脑 cDNA 克隆，它们在体外编码大型蛋白质。  |  Ishikawa, K., et al. 1997. DNA Res. 4: 307-13. PMID: 9455477

订购信息

产品名称产品编号规格价格数量收藏夹

MBNL2 抗体 (3B4)

sc-136167
200 µg/ml
$316.00

MBNL2 (3B4): m-IgG Fc BP-HRP 套装

sc-528802
200 µg Ab; 10 µg BP
$354.00

MBNL2 (3B4): m-IgGκ BP-HRP 套装

sc-521333
200 µg Ab, 40 µg BP
$354.00

MBNL2 抗体 (3B4) AC

sc-136167 AC
500 µg/ml, 25% agarose
$416.00

MBNL2 抗体 (3B4) HRP

sc-136167 HRP
200 µg/ml
$316.00

MBNL2 抗体 (3B4) FITC

sc-136167 FITC
200 µg/ml
$330.00

MBNL2 抗体 (3B4) PE

sc-136167 PE
200 µg/ml
$343.00

MBNL2 抗体 (3B4) Alexa Fluor® 488

sc-136167 AF488
200 µg/ml
$357.00

MBNL2 抗体 (3B4) Alexa Fluor® 546

sc-136167 AF546
200 µg/ml
$357.00

MBNL2 抗体 (3B4) Alexa Fluor® 594

sc-136167 AF594
200 µg/ml
$357.00

MBNL2 抗体 (3B4) Alexa Fluor® 647

sc-136167 AF647
200 µg/ml
$357.00

MBNL2 抗体 (3B4) Alexa Fluor® 680

sc-136167 AF680
200 µg/ml
$357.00

MBNL2 抗体 (3B4) Alexa Fluor® 790

sc-136167 AF790
200 µg/ml
$357.00

Do you know what the epitope sequence is that the Mbnl2 antibody recognizes? Or at least whether it recognizes the N- or C-terminal end of the protein?

Asked by: mmbhd
Thank you for your question. Anti-MBNL2 Antibody (3B4): sc-136167 was raised against recombinant MBNL2 of human origin. No epitope mapping has been performed for this antibody as of yet. Its exact epitope is not known.
Answered by: Tech Support Europe
Date published: 2022-02-17

Can this antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: cjMara
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. We recommend following our recommended protocol, provided below, https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining Alternatively, we do offer MBNL2 (3B4): sc-136167 monoclonal antibody directly conjugated to HRP, and a variety of fluorochromes, if you wish to omit using any secondary detection reagent and potential non-specific binding.
Answered by: Technical Support 1
Date published: 2017-01-26
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Rated 5 out of 5 by from Excellent results!This antiodies works really well with mouse brain homogenates.
Date published: 2020-03-23
Rated 5 out of 5 by from Very nice WesternI've used it for western blot in mouse brain tissue and the results are very nice!
Date published: 2018-02-13
Rated 5 out of 5 by from Good For Western BlotWe used this antibody in dilution of 1:500 to detect MBNL2 in human cancer cells, and we got good results!!!
Date published: 2017-05-20
Rated 5 out of 5 by from Good choice for WBUsed by WB, MBNL2 Antibody (3B4) very nice results
Date published: 2017-01-23
Rated 5 out of 5 by from Very cleanVery clean, strong, specific signal on western blots.
Date published: 2015-12-01
Rated 5 out of 5 by from Great Ab working better than the "scGreat Ab working better than the "sc-133774" Used it on primary culture of neuron
Date published: 2015-09-16
Rated 5 out of 5 by from Band detected at expected mw in CakiBand detected at expected mw in Caki-1, SK-N-SH, HeLa and U-87 MG whole cell lysates and mouse brain tissue extract. -SCBT QC
Date published: 2014-01-16
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MBNL2 抗体 (3B4) is rated 5.0 out of 5 by 7.
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