Laminin beta-1抗体 (A-1) 推荐用于 mouse, rat 和 human 来源的Laminin β-1 WB, IP, IF 和 IHC(P)检测

Laminin β-1 抗体 (A-1): sc-17810

Laminin β-1抗体 (A-1) is rated 4.3 out of 5 by 8.
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说明书

    • 抗-Laminin beta-1 抗体 A-1 是小鼠单克隆 IgG3 κ,Laminin β-1抗体, 在7篇文献中引用,规格为200 µg/ml
    • 免疫human来源Laminin β-1位于C-terminus 的1487-1786氨基酸
    • 推荐用于 mouse, rat 和 human 来源的Laminin β-1 WB, IP, IF 和 IHC(P)检测
    • 关于如何获取Laminin β-1 (A-1): sc-17810免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Laminin β-1 Antibody (A-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with Laminin β-1 Antibody (A-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the recommended fixation method for immunofluorescence staining with Laminin β-1 (A-1): sc-17810 monoclonal antibody?

Asked by: Germaine
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Did not work for western blot Used a 1:300 dilution for WB with Sum159, Caco-2, and 293T cell lysates. No bands were observed.
Date published: 2017-11-30
Rated 4 out of 5 by from Very good for IHC(Fr) Staining for PFA-fixed and frozen mouse bone marrow
Date published: 2017-07-19
Rated 4 out of 5 by from Works well on blots; see image We compared the A-1 mouse monoclonal antibody to laminin beta1 with the older LT3 rat monoclonal antibody using secondary antibodies to mouse and rat respectively. The tissue was a homogenate of mouse choroid plexus, a rich source of laminin beta1, that had been frozen and thawed. Both antibodies worked well, but both anti-rat IgG and the mouse kappa BP stained lower MW bands. They may be fragments of laminin beta1, as they seem to migrate above the molecular weights of heavy and light chains, but more testing is needed. We have not yet tested it for immunofluorescence.
Date published: 2016-12-15
Rated 5 out of 5 by from Produced superb immunofluorescence basement Produced superb immunofluorescence basement membrane and trabecular meshwork staining in normal mouse eye frozen section. -SCBT QC
Date published: 2015-04-16
Rated 5 out of 5 by from Robust signal in WB in NIH/3T3 whole cell lysates Robust signal in WB in NIH/3T3 whole cell lysates. -SCBT QC
Date published: 2015-03-15
Rated 5 out of 5 by from Strong WB band in rat PC12 cells Strong WB band in rat PC12 cells. -SCBT Publication Review
Date published: 2015-02-10
Rated 5 out of 5 by from Good publishable data in WB in mouse ES cells Good publishable data in WB in mouse ES cells. -SCBT Publication Review
Date published: 2015-01-22
Rated 5 out of 5 by from Good CoIP antibody in human PC Good CoIP antibody in human PC-3 cells. -SCBT Publication Review
Date published: 2015-01-02
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