Lamin B1抗体 (B-10) 推荐用于 mouse, rat 和 human 来源的Lamin B1 WB, IP, IF 和 ELISA检测

Lamin B1 抗体 (B-10): sc-374015

Lamin B1抗体 (B-10) is rated 4.5 out of 5 by 37.
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说明书

    • Lamin B1抗体(B-10)是小鼠单克隆IgG1 κ, 在149篇文献中引用,规格为200 µg/ml
    • 特异性抗原位于mouse物种的Lamin B11的C-terminus的氨基酸559-586之间
    • 抗-Lamin B1 抗体 (B-10) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Lamin B1
    • 抗-Lamin B1 抗体 (B-10) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取Lamin B1 (B-10): sc-374015免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for Lamin B1 Antibody (B-10) for WB applications. This reagent is now offered in a bundle with Lamin B1 Antibody (B-10) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Before using this Ab, dose the extraction of nuclear proteins require specific steps for efficient detection of lamin B1

Asked by: jaou
Thank you for your question. You can follow our recommended protocol, which can be found under this link: https://www.scbt.com/de/resources/protocols/western-immuno-blotting
Answered by: Technical Support Europe
Date published: 2020-10-28

I used to do ChIP by using anti-LaminB (M-20) sc-6217 with Dynabeads protein G, and it worked well. Which magnetic beads do you recommend for ChIP using Lamin B1 Antibody (B-10): sc-374015 ?

Asked by: Naoko
Thank you for your question. This Lamin B1 antibody should work with any anti-mouse IgG magnetic beads. Since you have protein G magnetic beads, this should work fine since protein G has a strong binding to mouse IgG1. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-05

I'm trying to use this Ab for IF in dermal fibroblasts, so far with no results. I use methanol fixation at -20C for 5 min, 0,3% triton RT 3min, FBS blocking for 30 min and primary Ab incubation at 4C ON. Should I do anything different for it to work?

Asked by: Sofiamp
Thank you for your question. A link to our recommended protocol for IF using this mouse monoclonal antibody, Lamin B1 (B-10): sc-374015, is provided below. We recommend using the primary antibody at a starting dilution of 1:50. If after consulting our protocol you continue to have negative results, please contact our Technical Service Department for help with troubleshooting the antibody. Please call our European Support office toll-free at 00800 4573 8000, email [email protected], or contact us by live chat. https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-11-21

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with Lamin B1 Antibody (B-10): sc-374015?

Asked by: cjMara
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 10
Date published: 2017-01-09
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Rated 4 out of 5 by from Useful for my purposes I asked this antibody as free trial and, after I used it, I decided to buy it because it is enough specific . The only negative aspect is is that I have other 2 spots under the main lane at 67 KDa.
Date published: 2020-12-23
Rated 5 out of 5 by from Brightest for IF I compared B-10 (sc-374015), A-11 (sc-377000) and 8D1 (sc-56144) for IF of both MEF wt and MEF Lmnb1-/-. B-10 is most brightest at 1:500 dilution / 4C overnight reaction after either -20C MeOH 10min and 4%PFA 15min fixation. MeOH fixed condition labels slightly other filaments in Lmnb1-/- background. So, under MeOH fixation conditions, even lower concentrations may be successful.
Date published: 2020-09-04
Rated 3 out of 5 by from Lamin-B1 in nuclear extract of glioblastoma cells Tested in whole cell lysate of glioblastoma primary cells and U-87MG cells (30ug of protein), antibody dilution 1:500 in TBS-T 0.1%.
Date published: 2020-03-25
Rated 5 out of 5 by from 功能很出色 实验室2个月之前购买的,特意用了很多次再来评论,还是很好用的,年后看实验情况再订购。。。。。。。。。
Date published: 2020-01-03
Rated 5 out of 5 by from Lamin antibody review Very satisfied. Used with mouse cells, did not test on other species. Only used it in vitro on our pre-osteoblast cell line. We saw satisfactory results and clear lamin expression when imaging.
Date published: 2019-09-17
Rated 5 out of 5 by from Five stars This antibody worked very well by Western blotting on nuclear extracts from human 293 cells.
Date published: 2018-07-25
Rated 3 out of 5 by from Not suitable for rat protein 1:200 diluted for wb of nuclear protein of rat heart,the band is quite weak
Date published: 2018-05-15
Rated 4 out of 5 by from Lamin B1 sample 67kDa Antibody works well in WB (1:300 O/N 4C). Sometimes two additional bands in HeLa whole cell lysate.
Date published: 2018-03-10
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