Date published: 2025-9-6
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Lamin B1 Antibody (A-11) - Western Blotting - Image 350704
Lamin B1 抗体 (A-11): sc-377000. NIH/3T3 (A), HeLa (B), MOLT-4 (C), CCRF-CEM (D) 和 MCF7 (E) 全细胞裂解液中 Lamin B1 表达的 Western 印迹分析.
Lamin B1 抗体 (A-11): sc-377000. NIH/3T3 (A), HeLa (B), MOLT-4 (C), CCRF-CEM (D) 和 MCF7 (E) 全细胞裂解液中 Lamin B1 表达的 Western 印迹分析.

Lamin B1 抗体 (A-11): sc-377000

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说明书
  • Lamin B1 抗体 A-11 是小鼠单克隆 IgG1 κ，Lamin B1抗体, 在110篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列401-490位于human物种的Lamin B1的C-terminus附近
  • Lamin B1 抗体 (A-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Lamin B1; 也和以下物种反应，包括： 和 equine, canine, bovine and porcine
  • 抗Lamin B1抗体（A-11）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Lamin B1 (A-11): sc-377000的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG1 BP-HRP 和 m-IgGκ BP-HRP 是 Lamin B1 抗体 (A-11) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Lamin B1 抗体 (A-11) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Lamin 抗体包括 Lamin A, Lamin B1, Lamin B2 和 Lamin C

Lamin B1抗体（A-11）是一种IgG1 κ小鼠单克隆Lamin B1抗体（也称为LMNB1抗体），可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的Lamin B1蛋白。Lamin B1抗体（A-11）可以以非共轭抗Lamin B1抗体的形式提供，也可以以多种共轭形式的抗Lamin B1抗体提供，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。已发现一种独特的半胱氨酸蛋白酶家族，其序列、结构和底物特异性与先前描述的任何蛋白酶家族均不同。这个家族被称为Ced-3/ICE，是凋亡机制的关键组成部分，能够破坏对细胞寿命至关重要的特定靶蛋白。核纤层蛋白作为核纤层的一部分，对维持核膜和细胞形态的完整性至关重要，核纤层是核膜内核质侧的一层纤维层，被认为能够为核膜提供框架，也可能与染色质相互作用。B型核纤层蛋白，如核纤层蛋白B1，会经历一系列修饰过程，如法尼基化和磷酸化。核纤层蛋白B1是一种由基因编码的含有586个氨基酸的蛋白质，当其基因发生突变时，会参与常染色体显性成人发病性白质营养不良（ADLD）的发病过程。ADLD是一种以小脑功能障碍和中枢神经系统对称性脱髓鞘为特征的疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Lamin B1 抗体 (A-11) 参考文献:

  1. 核片层蛋白的动态特性和可能功能。  |  Moir, RD., et al. 1995. Int Rev Cytol. 162B: 141-82. PMID: 8557486
  2. ICE-LAP3是草履虫细胞死亡蛋白Ced-3的哺乳动物同源物，在Fas和肿瘤坏死因子诱导的细胞凋亡过程中被激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 1621-5. PMID: 8576161
  3. ICE-LAP6 是 ICE/Ced-3 基因家族的一个新成员，它被细胞毒性 T 细胞蛋白酶颗粒酶 B 激活。  |  Duan, H., et al. 1996. J Biol Chem. 271: 16720-4. PMID: 8663294
  4. Mch2 alpha 而非 CPP32 对层粘蛋白 A 的裂解：多种白细胞介素 1 β-转化酶相关蛋白酶在细胞凋亡过程中具有不同的底物识别特性。  |  Takahashi, A., et al. 1996. Proc Natl Acad Sci U S A. 93: 8395-400. PMID: 8710882
  5. Mch4体外激活CPP32和Mch3，Mch4是一种新型人类凋亡半胱氨酸蛋白酶，含有两个类似FADD的结构域。  |  Fernandes-Alnemri, T., et al. 1996. Proc Natl Acad Sci U S A. 93: 7464-9. PMID: 8755496
  6. 凋亡过程中，板层蛋白酶解促进了核事件的发生。  |  Rao, L., et al. 1996. J Cell Biol. 135: 1441-55. PMID: 8978814

订购信息

产品名称产品编号规格价格数量收藏夹

Lamin B1 抗体 (A-11)

sc-377000
200 µg/ml
$316.00

Lamin B1 (A-11): m-IgGκ BP-HRP 套装

sc-523146
200 µg Ab, 40 µg BP
$354.00

Lamin B1 (A-11): m-IgG1 BP-HRP 套装

sc-533558
200 µg Ab; 20 µg BP
$354.00

Lamin B1 抗体 (A-11) AC

sc-377000 AC
500 µg/ml, 25% agarose
$416.00

Lamin B1 抗体 (A-11) HRP

sc-377000 HRP
200 µg/ml
$316.00

Lamin B1 抗体 (A-11) FITC

sc-377000 FITC
200 µg/ml
$330.00

Lamin B1 抗体 (A-11) PE

sc-377000 PE
200 µg/ml
$343.00

Lamin B1 抗体 (A-11) Alexa Fluor® 488

sc-377000 AF488
200 µg/ml
$357.00

Lamin B1 抗体 (A-11) Alexa Fluor® 546

sc-377000 AF546
200 µg/ml
$357.00

Lamin B1 抗体 (A-11) Alexa Fluor® 594

sc-377000 AF594
200 µg/ml
$357.00

Lamin B1 抗体 (A-11) Alexa Fluor® 647

sc-377000 AF647
200 µg/ml
$357.00

Lamin B1 抗体 (A-11) Alexa Fluor® 680

sc-377000 AF680
200 µg/ml
$357.00

Lamin B1 抗体 (A-11) Alexa Fluor® 790

sc-377000 AF790
200 µg/ml
$357.00

Cual seria el Ab 2° con HRP para este anticuerpo?

Asked by: Danihui
Thank you for your question. I would suggest the following secondary: sc-516102 mouse IgG kappa binding protein (m-IgGκ BP) conjugated to Horseradish Peroxidase (HRP) You can find it here: https://www.scbt.com/p/m-igg-kappa-bp-hrp
Answered by: Tech Service
Date published: 2020-02-18

Is this Lamin B1 Antibody (C-5): sc-365962 available directly conjugated to HRP or fluorescent dyes?

Asked by: cjMara
Thank you for your question. All requests for directly conjugated versions of this antibody should be directed to our Special Orders team at specialorders@scbt.com. Alternatively, consider using our Lamin B1 (A-11): sc-377000, which is available conjugated to agarose, HRP, FITC, PE, and more. (https://www.scbt.com/scbt/product/lamin-b1-antibody-a-11)
Answered by: Technical Support 13
Date published: 2017-11-03

Can Lamin B1 Antibody (A-11): sc-377000 be used for detection of Lamin B1 of monkey origin?

Asked by: jenni
Thank you for your inquiry. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity. We are available by phone, e-mail or chat.
Answered by: Technical Support 12
Date published: 2020-10-26
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Rated 5 out of 5 by from Great resultsGood results when tested Lamin B1 in WB and IF in human neutrophils.
Date published: 2024-03-27
Rated 5 out of 5 by from well function in WB and IFGood results when tested Lamin B1 in western blot of whole cell extracts or IFfrom rat articular chondrocytes.
Date published: 2023-12-22
Rated 5 out of 5 by from Lamin B1 (A-11) AntibodyThis is a clean antibody with detecting pro and C-term
Date published: 2022-09-07
Rated 4 out of 5 by from Ottimo anticorpoAnticorpo molto performante alla condizione 1:100 ph6 testato su brain murino.
Date published: 2021-06-21
Rated 1 out of 5 by from Not suitable for mouse IFI compared B-10 (sc-374015), A-11 (sc-377000) and 8D1 (sc-56144) for IF of both MEF wt and MEF Lmnb1-/-. A-11 is not capable of IF with mouse embryonic fibroblasts in our condition (neither -20C MeOH 10min or 4%PFA 15min fixation followed 1:500 dilution / 4C overnight reaction). I strongly recommend B-10 or 8D1 for IF of mouse cells.
Date published: 2020-09-04
Rated 5 out of 5 by from 核タンパクのローディングコントロールに最適です。核タンパクと細胞質を分離して、各々のウエスタンブロットを行った際に、核タンパクのローディングコントロールとして使用しましたが、きれいに分離できているのが確認できました。
Date published: 2020-07-06
Rated 5 out of 5 by from Great resultsBought this Ab conjugated to Alexa Fluor 647 for use in an IF assay using ImagestreamX and it worked reasonably well at 1:50 dilution. Despite being conjugated, we were also able to use the same Ab in a standard WB and at 1:500 dilution, we saw a strong, clear band in HL-60 nuclear lysate with no obvious hindrance from the fluor tag.
Date published: 2019-03-05
Rated 5 out of 5 by from Workes nicelyWorked perfectly for human and mouse cell lines at 1:1000 dilution (1:25000 secundary Ab)
Date published: 2019-02-11
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Lamin B1 抗体 (A-11) is rated 4.5 out of 5 by 23.
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