Date published: 2025-9-9

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L-Selectin/CD62L/SELL 抗体 (B-8): sc-390756

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说明书
  • L-Selectin/CD62L/SELL 抗体 B-8 是小鼠单克隆 IgG1 κ,L-Selectin/CD62L/SELL抗体, 在3篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列224-372位于human物种的L-Selectin的C-terminus
  • L-Selectin/CD62L/SELL 抗体 (B-8) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 L-Selectin
  • 抗L-Selectin/CD62L/SELL抗体(B-8)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取L-Selectin/CD62L/SELL (B-8): sc-390756免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG1 BP-HRPm-IgGκ BP-HRP 是 L-Selectin/CD62L/SELL 抗体 (B-8) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与L-Selectin/CD62L/SELL 抗体 (B-8) 组合成套装提供。 (请参阅下面的订购信息)。

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関連項目

L-选择素抗体(B-8)是一种IgG1 κ小鼠单克隆L-选择素抗体(也称为L-选择素抗体),可通过WB、IP、IF、IHC(P)和ELISA检测小鼠、大鼠和人类来源的L-选择素蛋白。L-选择素抗体(B-8)既可作为非偶联的抗L-选择素抗体形式提供,也可作为多种偶联形式的抗L-选择素抗体提供,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。选择素,也被称为CD62抗原,是一个由与介导白细胞细胞间相互作用有关的碳水化合物结合蛋白组成的家族。L-选择素(也被称为LECAM-1或CD62L)在大多数B细胞和初始T细胞以及大多数单核细胞、中性粒细胞和嗜酸性粒细胞上表达。L-选择素与活化的内皮细胞表达的特定碳水化合物相互作用。P-选择素(也被称为GMP-140或CD62P)在活化的血小板和内皮细胞上表达,而E-选择素(也被称为ELMA-1或CD62E)在内皮细胞上表达,它们表现出重叠的配体特异性。两者都识别sialyl-Lex作为配体,并与中性粒细胞和单核细胞上的特定碳水化合物结合。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

L-Selectin/CD62L/SELL 抗体 (B-8) 参考文献:

  1. 糖皮质激素对多形核白细胞上L-选择素表达的影响。  |  Nakagawa, M., et al. 1999. Blood. 93: 2730-7. PMID: 10194453
  2. 在体内急性炎症反应期间,L-选择素促进白细胞的迁移和血管外运动。  |  Hickey, MJ., et al. 2000. J Immunol. 165: 7164-70. PMID: 11120848
  3. 肺循环中中性粒细胞对fMLP的边缘化需要L-选择素,但不需要C5a。  |  Olson, TS., et al. 2002. Am J Physiol Regul Integr Comp Physiol. 282: R1245-52. PMID: 11893631
  4. 细胞骨架相互作用调节诱导性L-选择素聚集。  |  Mattila, PE., et al. 2005. Am J Physiol Cell Physiol. 289: C323-32. PMID: 15788481
  5. 过敏患者中性粒细胞上的L-选择素表达。  |  Monteseirín, J., et al. 2005. Clin Exp Allergy. 35: 1204-13. PMID: 16164449
  6. FOXO1通过与FOXO1的共识序列结合上调人类L-选择素表达。  |  Lou, Y., et al. 2012. Gene Regul Syst Bio. 6: 139-49. PMID: 23133314
  7. PSGL-1-L-选择素信号复合物调节流动状态下中性粒细胞的粘附。  |  Stadtmann, A., et al. 2013. J Exp Med. 210: 2171-80. PMID: 24127491
  8. 中性粒细胞与E-选择素的结合和滚动可通过L-选择素的需求来分离。  |  Lawrence, MB., et al. 1994. Immunity. 1: 137-45. PMID: 7534197
  9. L-选择素与碳水化合物的相互作用:Lewis x三糖的相关修饰。  |  Sanders, WJ., et al. 1996. Biochemistry. 35: 14862-7. PMID: 8942649
  10. L-选择素通过激活小G蛋白Rac2来调节肌动蛋白聚合。  |  Brenner, B., et al. 1997. Biochem Biophys Res Commun. 231: 802-7. PMID: 9070897

订购信息

产品名称产品编号规格价格数量收藏夹

L-Selectin/CD62L/SELL 抗体 (B-8)

sc-390756
200 µg/ml
$316.00

L-Selectin/CD62L/SELL (B-8): m-IgG Fc BP-HRP 套装

sc-530252
200 µg Ab; 10 µg BP
$354.00

L-Selectin/CD62L/SELL (B-8): m-IgGκ BP-HRP 套装

sc-523669
200 µg Ab, 40 µg BP
$354.00

L-Selectin/CD62L/SELL (B-8): m-IgG1 BP-HRP 套装

sc-543959
200 µg Ab; 20 µg BP
$354.00

L-Selectin/CD62L/SELL 抗体 (B-8) AC

sc-390756 AC
500 µg/ml, 25% agarose
$416.00

L-Selectin/CD62L/SELL 抗体 (B-8) HRP

sc-390756 HRP
200 µg/ml
$316.00

L-Selectin/CD62L/SELL 抗体 (B-8) FITC

sc-390756 FITC
200 µg/ml
$330.00

L-Selectin/CD62L/SELL 抗体 (B-8) PE

sc-390756 PE
200 µg/ml
$343.00

L-Selectin/CD62L/SELL 抗体 (B-8) Alexa Fluor® 488

sc-390756 AF488
200 µg/ml
$357.00

L-Selectin/CD62L/SELL 抗体 (B-8) Alexa Fluor® 546

sc-390756 AF546
200 µg/ml
$357.00

L-Selectin/CD62L/SELL 抗体 (B-8) Alexa Fluor® 594

sc-390756 AF594
200 µg/ml
$357.00

L-Selectin/CD62L/SELL 抗体 (B-8) Alexa Fluor® 647

sc-390756 AF647
200 µg/ml
$357.00

L-Selectin/CD62L/SELL 抗体 (B-8) Alexa Fluor® 680

sc-390756 AF680
200 µg/ml
$357.00

L-Selectin/CD62L/SELL 抗体 (B-8) Alexa Fluor® 790

sc-390756 AF790
200 µg/ml
$357.00

I am using L-Selectin (B-8): sc-390756 monoclonal antibody to stain paraffin embedded tissue. Do you recommend antigen retrieval when using this antibody?

Asked by: DefinitelyNotMatt
We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_8, d_27, h_5CST
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Rated 5 out of 5 by from Great for immunohistochemical analysisWe used this antibody to bind the L-selectin of TAMs for immunohistochemical analysis. And it worked very well and the result was prefect.
Date published: 2017-12-04
Rated 5 out of 5 by from WB on human aortic smooth muscle cellsWe ordered the sample antibody, and tested on human VSMCs, and it gives good WB results.
Date published: 2017-04-02
Rated 5 out of 5 by from Produced excellent immunoperoxidase membraneProduced excellent immunoperoxidase membrane and cytoplasmic staining in formalin fixed, paraffin-embedded human appendix tissue. -SCBT QC
Date published: 2015-01-16
Rated 5 out of 5 by from Good Western Blot data of LGood Western Blot data of L-Selectin expression in Jurkat and HL-60 whole cell lysates. -SCBT QC
Date published: 2013-03-18
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L-Selectin/CD62L/SELL 抗体 (B-8) is rated 5.0 out of 5 by 4.
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