Jun B抗体 (C-11) 推荐用于 mouse, rat 和 human 来源的Jun B WB, IP, IF, IHC(P) 和 ELISA检测

Jun B 抗体 (C-11): sc-8051

Jun B抗体 (C-11) is rated 4.3 out of 5 by 10.
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说明书

    • 抗-Jun B 抗体 C-11 是小鼠单克隆 IgG1 (kappa轻链),Jun B抗体,规格为200 µg/ml
    • 特异性抗原位于mouse物种的Jun B的C-terminus附近的氨基酸210-222之间
    • 抗-Jun B 抗体 (C-11) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Jun B
    • 抗-Jun B 抗体 (C-11) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联TRITC 以及 Alexa Fluor® 405 用于IF, IHC(P) 和 FCM
    • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8051 X, 200 µg/0.1 ml)
    • 封闭肽,sc-8051 P
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取Jun B (C-11): sc-8051免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。

Can you recommend a negative control to use with Jun B Antibody (C-11): sc-8051?

Asked by: jenni
We recommend using the blocking peptide sc-8051 P as a negative control. Antibody binding to antigen may be blocked/competed by pre-absorption with the blocking peptide. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support 4
Date published: 2017-01-09
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Rated 3 out of 5 by from SDS-PAGE It worked OK, BUT it did not significantly better than previous one
Date published: 2018-03-30
Rated 4 out of 5 by from Worked good I used on Western Blot for NIH3T3 cells with 1:200 dilution,worked good.
Date published: 2017-12-27
Rated 4 out of 5 by from Must use Santa Cruz secondary Ab We used this antibody several years ago. We requested a new sample and tested on HEK-293 cell lysates using a 1:500 dilution. We used Jackson Immuno Research anti-mouse IgG AF-680 secondary and imaged on the Licor Odyssey imager. No signal was detected. We requested a new sample along with the Santa Cruz secondary Ab (AF-680) that is specific for m-IgG Kappa Light Chain. It showed a nice band just under the 37 kD marker. Generally we have found that although whole IgG abs should still recognize these new mouse monoclonals, we get much better signal with the m-IgG Kappa Light Chain secondaries for most sample abs we have tested in both IHC and WB.
Date published: 2017-10-12
Rated 2 out of 5 by from cytoplasm staining Produced immunofluorescence cytoplasm staining in methanol-fixed HK2 cells. why?
Date published: 2017-08-18
Rated 5 out of 5 by from Outstanding product Strong product for multiple applications; best available jun b antibody
Date published: 2017-01-10
Rated 5 out of 5 by from Good Antibody for IHC Versatile antibody showing great staining in human tissue samples as well as strong signal in WB and ELISA assays. Usable in human, mouse, and rat samples for a variety of applications.
Date published: 2016-12-20
Rated 5 out of 5 by from Produced beautiful immunofluorescence nuclear Produced beautiful immunofluorescence nuclear staining in methanol-fixed KNRK cells. -SCBT QC
Date published: 2015-05-01
Rated 5 out of 5 by from Worked great in our blot Worked great in our blot. We tested human
Date published: 2015-04-09
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