Date published: 2025-9-6
访问圣克鲁斯动物健康

021-6093-6350

SCBT Portrait Logo
Seach Input
联系我们
登陆账户
快速订购
购物车 (0)
p-c-Jun Antibody (KM-1) - Western Blotting - Image 133105
未经处理 (A, D), 紫外线照射 (B, E) 和紫外线照射及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 NIH/3T3 全细胞裂解液中 c-Jun 磷酸化的 Western 印迹分析. 测试的抗体包括 p-c-Jun 抗体 (KM-1): sc-822 (A, B, C) 和 c-Jun (H-79): sc-1694 (D, E, F).
未经处理 (A, D), 紫外线照射 (B, E) 和紫外线照射及λ蛋白磷酸酶 (sc-200312A) 处理 (C, F) 的 NIH/3T3 全细胞裂解液中 c-Jun 磷酸化的 Western 印迹分析. 测试的抗体包括 p-c-Jun 抗体 (KM-1): sc-822 (A, B, C) 和 c-Jun (H-79): sc-1694 (D, E, F).

p-c-Jun 抗体 (KM-1): sc-822

4.7(38)
寫評論提問

说明书
  • p-c-Jun抗体(KM-1)是小鼠单克隆IgG1 κ, 在590篇文献中引用，规格为200 µg/ml
  • p-c-Jun 抗体 (KM-1) 推荐用于WB, IP, IF, IHC(P) 和 FCM ，检测 mouse, rat, human 和 来源的c-Jun p39 phosphorylated on Serine 63; 也和以下物种反应，包括： 和 canine, bovine, porcine and avian; 不会与 Jun B or Jun D phosphorylated on the analogous serine residues or with c-Jun non-phosphorylated at Serine 63产生交联反应
  • 抗p-c-Jun抗体（KM-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-822 X, 200 µg/0.1 ml)
  • 关于如何获取p-c-Jun (KM-1): sc-822的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 p-c-Jun 抗体 (KM-1) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与p-c-Jun 抗体 (KM-1) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

相关产品
描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 Jun 抗体 包括 c-Jun, Jun B和Jun D

p-c-Jun抗体（KM-1）是一种小鼠单克隆IgG1 kappa轻链抗体，可通过应用诸如蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和流式细胞术（FCM）等方法检测小鼠、大鼠和人类样本中丝氨酸63位点磷酸化的小鼠c-Jun蛋白。p-c-Jun（KM-1）抗体 有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。c-Jun蛋白在细胞过程中起着至关重要的作用，是转录因子AP-1的关键组成部分，可调节基因表达以应对各种刺激，包括生长因子和应激信号。这种调节对于细胞增殖、分化、凋亡等过程至关重要，因此c-Jun在肿瘤发生和细胞对外部信号的反应中扮演着重要角色。c-Jun与Fos蛋白形成异源二聚体，从而增强转录活性，使细胞能够对环境信号做出更强大的反应。此外，c-Jun在丝氨酸63位上的磷酸化是一种关键的修饰，可增强转录活性，与包括Ras激活的信号通路在内的各种信号通路相关联。了解c-Jun的动态变化和相互作用对于阐明许多细胞功能和疾病状态的基本机制至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

p-c-Jun 抗体 (KM-1) 参考文献:

  1. t(8;21) 融合蛋白 AML1/ETO 能转化 NIH3T3 细胞并激活 AP-1。  |  Frank, RC., et al. 1999. Oncogene. 18: 1701-10. PMID: 10208431
  2. JNK依赖性c-Jun丝氨酸63位磷酸化介导一氧化氮诱导的神经母细胞瘤细胞凋亡。  |  Li, L., et al. 2004. J Biol Chem. 279: 4058-65. PMID: 14617628
  3. IP-1：Fos/Jun 的主要抑制剂，其活性受磷酸化调节。  |  Auwerx, J. and Sassone-Corsi, P. 1991. Cell. 64: 983-93. PMID: 1900458
  4. Ha-Ras 可增强 c-Jun 的活性并刺激其活化结构域的磷酸化。  |  Binétruy, B., et al. 1991. Nature. 351: 122-7. PMID: 1903181
  5. 人类原癌基因 c-jun 编码一种 DNA 结合蛋白，具有转录因子 AP-1 的结构和功能特性。  |  Bohmann, D., et al. 1987. Science. 238: 1386-92. PMID: 2825349
  6. Fos 复合物和 Fos 相关抗原可识别包含 AP-1 结合位点的序列元素。  |  Franza, BR., et al. 1988. Science. 239: 1150-3. PMID: 2964084
  7. c-fos 原癌基因产物的染色质关联和 DNA 结合特性。  |  Renz, M., et al. 1987. Nucleic Acids Res. 15: 277-92. PMID: 3103098
  8. 癌基因 jun 编码一种类似于 AP-1 的序列特异性反式激活因子。  |  Angel, P., et al. 1988. Nature. 332: 166-71. PMID: 3347253
  9. Fos 蛋白复合物与离体细胞核中的 DNA 相关联，并与 DNA 纤维素结合。  |  Sambucetti, LC. and Curran, T. 1986. Science. 234: 1417-9. PMID: 3491427
  10. 调节脂肪细胞分化过程中基因表达的核蛋白复合物：c-fos 的直接参与。  |  Distel, RJ., et al. 1987. Cell. 49: 835-44. PMID: 3555845

订购信息

产品名称产品编号规格价格数量收藏夹

p-c-Jun 抗体 (KM-1)

sc-822
200 µg/ml
$316.00

p-c-Jun (KM-1): m-IgG Fc BP-HRP 套装

sc-526463
200 µg Ab; 10 µg BP
$354.00

p-c-Jun (KM-1): m-IgG1 BP-HRP 套装

sc-531836
200 µg Ab; 20 µg BP
$354.00

p-c-Jun 抗体 (KM-1) X

sc-822 X
200 µg/0.1 ml
$316.00

p-c-Jun 抗体 (KM-1) AC

sc-822 AC
500 µg/ml, 25% agarose
$416.00

p-c-Jun 抗体 (KM-1) HRP

sc-822 HRP
200 µg/ml
$316.00

p-c-Jun 抗体 (KM-1) FITC

sc-822 FITC
200 µg/ml
$330.00

p-c-Jun 抗体 (KM-1) PE

sc-822 PE
200 µg/ml
$343.00

p-c-Jun 抗体 (KM-1) Alexa Fluor® 488

sc-822 AF488
200 µg/ml
$357.00

p-c-Jun 抗体 (KM-1) Alexa Fluor® 546

sc-822 AF546
200 µg/ml
$357.00

p-c-Jun 抗体 (KM-1) Alexa Fluor® 594

sc-822 AF594
200 µg/ml
$357.00

p-c-Jun 抗体 (KM-1) Alexa Fluor® 647

sc-822 AF647
200 µg/ml
$357.00

p-c-Jun 抗体 (KM-1) Alexa Fluor® 680

sc-822 AF680
200 µg/ml
$357.00

p-c-Jun 抗体 (KM-1) Alexa Fluor® 790

sc-822 AF790
200 µg/ml
$357.00

My results always show multiple bands (2-3) for p-c-Jun even though there is only 1 stated MW of 39kDa, may I know if it is due to unspecific binding or presence of isomers?

Asked by: olivia0183
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2022-05-10

Is there any data to support the fact that this antibody is specific for phosphorylated c-Jun?

Asked by: AbPolly
Thank you for your inquiry. Yes, in Western Blot we see upregulation of signal after the sample is treated with UV radiation, and downregulation of signal following treatment with lambda phosphatase.
Answered by: Technical Service
Date published: 2019-11-08

What is the recommended blocking buffer to use with this antibody in Western Blot?

Asked by: AbPolly
Thank you for your question. When detecting phosphorylated proteins in Western Blot, we recommend using Blotto B as the blocking buffer, sc-2335.
Answered by: Technical Service
Date published: 2016-12-22
  • y_2025, m_8, d_28, h_5CST
  • bvseo_bulk, prod_bvqa, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasquestionsanswers, tq_3
  • loc_zh_CN, sid_822, prod, sort_[SortEntry(order=LAST_APPROVED_ANSWER_SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getContent, 127ms
  • QUESTIONS, PRODUCT
Rated 5 out of 5 by from Good work for WBThe p-c-Jun antibody works well in hepatic mouse cells
Date published: 2024-07-05
Rated 5 out of 5 by from Good work for WBThe p-c-Jun (S63) antibody was working very well in human cardiac fibroblast cells.
Date published: 2022-11-02
Rated 5 out of 5 by from p-c-Jun antibodyThe p-c-Jun (S63) antibody was working very well in our WB.
Date published: 2021-07-07
Rated 4 out of 5 by from Good for WBThis antibody is high specific and high sensitivity in human liver cell lines. Highly recommend antibody.
Date published: 2019-11-02
Rated 4 out of 5 by from excellentThis antibody is high specific and high sensitivity in human cell lines. Highly recommend this antibody
Date published: 2019-10-23
Rated 4 out of 5 by from Great for detection of phosphorylated C-JunThis antibody worked well for the detection of downstream P-C-Jun in the murine macrophage cell line RAW 246. It has minimal background.
Date published: 2018-12-17
Rated 2 out of 5 by from It does not work wellWe test this ab by 1:500 dilution, and it did not work out well
Date published: 2018-04-03
Rated 4 out of 5 by from good antibodyI received this free sample 2 months ago and tested it by Western blot. It shows clean bands in both whole cell lysate and nuclear extract.
Date published: 2018-01-04
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_38
  • loc_zh_CN, sid_822, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getReviews, 25ms
  • REVIEWS, PRODUCT
p-c-Jun 抗体 (KM-1) is rated 4.7 out of 5 by 38.
  • y_2025, m_8, d_28, h_5
  • bvseo_bulk, prod_bvrr, vn_bulk_3.0.42
  • cp_1, bvpage1
  • co_hasreviews, tv_0, tr_38
  • loc_zh_CN, sid_822, prod, sort_[SortEntry(order=SUBMISSION_TIME, direction=DESCENDING)]
  • clientName_scbt
  • bvseo_sdk, java_sdk, bvseo-4.0.0
  • CLOUD, getAggregateRating, 111ms
  • REVIEWS, PRODUCT
021-6093-6350
访问 Santa Cruz Animal Health|访问 San Juan Ranch
Santa Cruz Biotechnology, Inc. 是生物医学研究市场产品开发的世界领导者。请拨打0086-021-60936350联系我们。
版权所有© 2007-2025 Santa Cruz Biotechnology, Inc. 保留所有权利。”Santa Cruz Biotechnology” 和Santa Cruz Biotechnology, Inc.的商标，”Santa Cruz Animal Health”, “San Juan Ranch”, “Supplement of Champions”, San Juan Ranch的商标，”UltraCruz”, “ChemCruz”, “ImmunoCruz”, “ExactaCruz”, 和 “EZ Touch”是Santa Cruz Biotechnology, Inc.的注册商标。所有商标均为其各自所有者的财产。