Date published: 2025-9-5

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IRF-1 抗体 (E-4): sc-514544

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说明书
  • IRF-1 抗体 E-4 是小鼠单克隆 IgG2a κ,IRF-1抗体, 在19篇文献中引用,规格为200 µg/ml
  • 特异性抗原位于mouse物种的IRF-1的C-terminus的氨基酸307-328之间
  • IRF-1 抗体 (E-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 IRF-1
  • 抗IRF-1抗体(E-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-514544 X, 200 µg/0.1 ml)
  • 关于如何获取IRF-1 (E-4): sc-514544免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是IRF-1 Antibody (E-4) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与IRF-1 Antibody (E-4) 打包提供(请参阅下面的订购信息)。

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関連項目

IRF-1抗体(E-4)是一种IgG2a κ小鼠单克隆IRF-1抗体(也称为IRF1抗体),可通过WB、IP、IF和ELISA检测小鼠、大鼠和人的IRF-1蛋白。IRF-1抗体(E-4)既可以是非偶联的抗IRF-1抗体形式,也可以是多种偶联形式的抗IRF-1抗体,包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。干扰素调节因子-1(IRF-1)和IRF-2已被鉴定为新型的DNA结合因子,它们作为I型干扰素(干扰素-α和β)和干扰素诱导基因的调节因子发挥作用。这两个因子在结构上相关,特别是在它们的N端区域,该区域赋予DNA结合特异性。此外,它们都与干扰素-α和干扰素-β基因启动子内的相同序列结合。IRF-1作为干扰素转录的激活剂发挥作用,而IRF-2则与相同的顺式元件结合并抑制IRF-1的作用。据报道,IRF-1和IRF-2在调节细胞生长方面以相互拮抗的方式发挥作用;抑制因子IRF-2的过表达导致细胞转化,而IRF-1的同时过表达则引起逆转。IRF-1和IRF-2是更大的DNA结合蛋白家族的成员,该家族包括IRF-3、IRF-4、IRF-5、IRF-6、IRF-7、ISGF-3γ p48(ISGF-3复合物的组分)和IFN共识序列结合蛋白(ICSBP)。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

IRF-1 抗体 (E-4)

sc-514544
200 µg/ml
$316.00

IRF-1 (E-4): m-IgG Fc BP-HRP 套装

sc-531156
200 µg Ab; 10 µg BP
$354.00

IRF-1 (E-4): m-IgGκ BP-HRP 套装

sc-524762
200 µg Ab, 40 µg BP
$354.00

IRF-1 (E-4): m-IgG2a BP-HRP 套装

sc-547707
200 µg Ab; 10 µg BP
$354.00

IRF-1 抗体 (E-4) X

sc-514544 X
200 µg/0.1 ml
$316.00

IRF-1 抗体 (E-4) AC

sc-514544 AC
500 µg/ml, 25% agarose
$416.00

IRF-1 抗体 (E-4) HRP

sc-514544 HRP
200 µg/ml
$316.00

IRF-1 抗体 (E-4) FITC

sc-514544 FITC
200 µg/ml
$330.00

IRF-1 抗体 (E-4) PE

sc-514544 PE
200 µg/ml
$343.00

IRF-1 抗体 (E-4) Alexa Fluor® 488

sc-514544 AF488
200 µg/ml
$357.00

IRF-1 抗体 (E-4) Alexa Fluor® 546

sc-514544 AF546
200 µg/ml
$357.00

IRF-1 抗体 (E-4) Alexa Fluor® 594

sc-514544 AF594
200 µg/ml
$357.00

IRF-1 抗体 (E-4) Alexa Fluor® 647

sc-514544 AF647
200 µg/ml
$357.00

IRF-1 抗体 (E-4) Alexa Fluor® 680

sc-514544 AF680
200 µg/ml
$357.00

IRF-1 抗体 (E-4) Alexa Fluor® 790

sc-514544 AF790
200 µg/ml
$357.00

IRF-1 (E-4) 中和勝肽

sc-514544 P
100 µg/0.5 ml
$68.00

I'm looking for an IRF-1 antibody that will react with mouse and/or rat. Am I correct that this is the only one that has been tested on mouse-originated cells and worked? Thank you!

Asked by: IRF1 antibody
Thank you for your question. This antibody is an excellent choice for detection of IRF-1 in mouse, rat and human samples. We also have two other IRF-1 antibodies that are recommended for mouse and rat and have data in mouse cell lines: IRF-1 Antibody (F-2): sc-514505 and IRF-1 Antibody (F-2): sc-514505.
Answered by: Technical Support
Date published: 2019-05-13

What application is the blocking peptide sc-514544 P appropriate for?

Asked by: Germaine
Thank you for your question. The blocking peptide is intended for use as a negative control, by pre-adsorbing the mouse monoclonal antibody against the antigen. For full protocol details, please contact our Technical Services Department or view our online protocol here: https://www.scbt.com/scbt/resources/protocols/peptide-neutralization
Answered by: Technical Support
Date published: 2017-02-27
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Rated 4 out of 5 by from WB Detectó correctamenteWestern blot: La dilución probada fue de 1:1000 con 20 ug de proteína de celulas CRFK, y detectó correctamente una banda aprox 40-45 kDa
Date published: 2024-04-01
Rated 3 out of 5 by from Works well for western blotting but not ChIPDisappointed that the only antibody, C-20 sc-497, was discontinued since I performed a Chromatin immunoprecipitation experiment in parallel comparing this and the discontinued antibody and this one did not work at all compared to getting 5 fold enrichment of regions known to bind IRF1 with the old antibody.
Date published: 2023-10-19
Rated 3 out of 5 by from Good workThis antibody worked well with fixed rat tissue!!!
Date published: 2022-07-05
Rated 4 out of 5 by from 功能不错~这次评论晚了抱歉,IRF1曝光出来条带清楚细腻,大概在不到50kd的位置,特异性也好,整体效果不错~有图有真相~!
Date published: 2017-11-08
Rated 5 out of 5 by from great for WB1:500 dilution in 5% BSA. RT 2h. Faint non-specific bands. Only tested for BV2 cell.
Date published: 2017-10-07
Rated 4 out of 5 by from 功能很出色Produced beautiful immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells
Date published: 2017-09-22
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IRF-1 抗体 (E-4) is rated 3.8 out of 5 by 6.
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