insulin R beta抗体 (C-4) 推荐用于 mouse, rat 和 human 来源的insulin Rβ WB, IP, IF 和 ELISA检测

insulin Rβ 抗体 (C-4): sc-373975

insulin Rβ抗体 (C-4) is rated 4.7 out of 5 by 6.
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说明书

    • insulin Rβ抗体(C-4)是小鼠单克隆IgG2a κ, 在3篇文献中引用,规格为200 µg/ml
    • 免疫human物种的insulin Rβ的氨基酸941-1010
    • 抗-insulin R beta 抗体 (C-4) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 insulin Rβ; 也和以下物种反应,包括: 和 equine, canine and porcine
    • 抗-insulin R beta 抗体 (C-4) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取insulin Rβ (C-4): sc-373975免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for insulin Rβ Antibody (C-4) for WB applications. This reagent is now offered in a bundle with insulin Rβ Antibody (C-4) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the reason that this antibody recognises ~130 kDa protein, while other Santa Cruz antibodies vs. Insulin Receptor beta reveal a band around ~95 kDa?

Asked by: JulP
Thank you for your question. The 130 kDa processed insulin Rβ as detected by insulin Rβ Antibody (C-4): sc-373975 is described in PMID 2187866 “Processed 130-kDa alpha and 95-kDa beta subunits were also observed and contained approximately 20% as much protein as the proreceptor on a molar basis.” Please contact technical service for more information.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

What is the recommended starting dilution for Western blot for sc-373975: insulin Rβ (C-4) monoclonal antibody?

Asked by: AbPolly
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 5 out of 5 by from Excellent I brought this product and i got excellent results. also the customer service and tech support are helpful
Date published: 2019-12-11
Rated 4 out of 5 by from No positivity in S. aurata and D. labrax tissues At the tested dilution (1:50, 1:200) and with the protocol in use in our laboratories, it was not possible to detect any positivity in Sparus aurata and Dicentrarchus labrax tissues
Date published: 2019-07-03
Rated 4 out of 5 by from IF on mouse mammary cancer cell Antibody – Santa Cruz 373975 anti-Insulin receptor beta Cell – mouse mammary cancer cell IF tested – dilution 1:50
Date published: 2017-12-18
Rated 5 out of 5 by from Excellent antibody The insulin R beta Antibody (C-4): sc-373975 works very well for detection of Insulin R via Western blot, showing strong and clean signal.
Date published: 2017-02-09
Rated 5 out of 5 by from Works well for WB in HeLa and JAR whole cell Works well for WB in HeLa and JAR whole cell lysates. -SCBT QC
Date published: 2015-01-17
Rated 5 out of 5 by from Strong band observed in western blot analysis Strong band observed in western blot analysis of insulin R expression in Hep G2 whole cell lysate. -SCBT QC
Date published: 2013-12-04
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