Date published: 2025-9-7
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INSR/Insulin Receptor β Antibody (CT-3) - Western Blotting - Image 393025
胰岛素 Rβ 抗体 (CT-3) HRP: sc-57342 HRP. NIH/3T3 (A), SW480 (B), MCF7 (C), JAR (D), MIA PaCa-2 (E) 和 Hep G2 (F) 全细胞裂解液中胰岛素 Rβ 表达的直接 Western 印迹分析.
胰岛素 Rβ 抗体 (CT-3) HRP: sc-57342 HRP. NIH/3T3 (A), SW480 (B), MCF7 (C), JAR (D), MIA PaCa-2 (E) 和 Hep G2 (F) 全细胞裂解液中胰岛素 Rβ 表达的直接 Western 印迹分析.

INSR/Insulin Receptor β 抗体 (CT-3): sc-57342

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寫評論提問

说明书
  • INSR/Insulin Receptor β 抗体 CT-3 是小鼠单克隆 IgG1 κ，INSR/Insulin Receptor β抗体, 在64篇文献中引用，规格为200 µg/ml
  • 针对insulin receptor的human起源的C-terminus的
  • INSR/Insulin Receptor β 抗体 (CT-3) 推荐用于 WB, IP, IF 和 IHC(P)，检测mouse, rat 和human 来源的 insulin Rβ; 不会与 insulin-like growth factor (IGF)receptors产生交联反应
  • 抗INSR/Insulin Receptor β抗体（CT-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取INSR/Insulin Receptor β (CT-3): sc-57342的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG1 BP-HRP 是 INSR/Insulin Receptor β 抗体 (CT-3) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与INSR/Insulin Receptor β 抗体 (CT-3) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 insulin R 抗体 包括 insulin R β

胰岛素 Rβ 抗体（CT-3）是一种小鼠单克隆 IgG1 kappa kappa 轻链抗体，专门设计用于靶向人胰岛素受体 beta 亚基的 C-末端。胰岛素 Rβ 单克隆抗体 (CT-3) 可在多种应用中可靠地检测来自人、小鼠和大鼠的胰岛素受体 beta 蛋白，包括免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和石蜡包埋切片免疫组化 (IHCP)，同时与胰岛素样生长因子 (IGF) 受体无交叉反应，确保了精确和特异性结合。胰岛素受体（IR）本身是一种重要的异源二聚体蛋白复合物，由细胞外的α亚基和跨膜的β亚基通过二硫键连接而成。与胰岛素结合后，IR 细胞内的酪氨酸激酶结构域会发生自身磷酸化，从而激活一系列信号蛋白，如 IRS-1、IRS-2、PI3K 和 GRB2。这些相互作用启动了调节葡萄糖摄取、糖原合成以及细胞生长和新陈代谢各个方面的关键分子途径。通过靶向β亚基，抗胰岛素 Rβ 抗体（CT-3）有助于研究这些错综复杂的信号转导机制和蛋白质相互作用，为深入了解胰岛素信号转导途径提供了宝贵的资料。了解 IR 的作用对于研究代谢性疾病至关重要，如 1 型糖尿病（一种导致胰岛素分泌细胞破坏的自身免疫性疾病）和 2 型糖尿病（以细胞对胰岛素作用的抵抗为特征）。胰岛素 Rβ (CT-3) 单克隆抗体是研究人员探索胰岛素信号转导的分子途径和蛋白质相互作用及其对各种疾病的影响的重要工具。胰岛素 Rβ (CT-3) 抗体有非共轭型和多种共轭型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和各种 Alexa Fluor® 共轭物。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

INSR/Insulin Receptor β 抗体 (CT-3) 参考文献:

  1. 通过胰岛素受体发出信号。  |  Whitehead, JP., et al. 2000. Curr Opin Cell Biol. 12: 222-8. PMID: 10712920
  2. 胰岛素受体：从蛋白质序列到结构。  |  Marino-Buslje, C., et al. 1999. Biochem Soc Trans. 27: 715-26. PMID: 10917671
  3. 跨膜信号传递机制：胰岛素结合与胰岛素受体。  |  Ottensmeyer, FP., et al. 2000. Biochemistry. 39: 12103-12. PMID: 11015187
  4. 胰岛素受体--结构和功能特征。  |  Perz, M. and Torlińska, T. 2001. Med Sci Monit. 7: 169-77. PMID: 11208515
  5. 胰岛素受体变异形式与 2 型糖尿病。  |  Sesti, G. 2000. Pharmacogenomics. 1: 49-61. PMID: 11258596
  6. 脂肪组织中缺乏胰岛素受体的小鼠寿命延长。  |  Blüher, M., et al. 2003. Science. 299: 572-4. PMID: 12543978
  7. 神经系统中的胰岛素信号调节黑腹果蝇的乙醇中毒。  |  Corl, AB., et al. 2005. Nat Neurosci. 8: 18-9. PMID: 15592467
  8. 缺乏建筑因子 HMGA1 会导致人类和小鼠出现胰岛素抵抗和糖尿病。  |  Foti, D., et al. 2005. Nat Med. 11: 765-73. PMID: 15924147
  9. 胰岛素受体的前三个结构域在结构上与胰岛素样生长因子 1 受体的配体特异性区域不同。  |  Lou, M., et al. 2006. Proc Natl Acad Sci U S A. 103: 12429-34. PMID: 16894147

订购信息

产品名称产品编号规格价格数量收藏夹

INSR/Insulin Receptor β 抗体 (CT-3)

sc-57342
200 µg/ml
$316.00

INSR/Insulin Receptor β (CT-3): m-IgG Fc BP-HRP 套装

sc-526895
200 µg Ab; 10 µg BP
$354.00

INSR/Insulin Receptor β (CT-3): m-IgG1 BP-HRP 套装

sc-532268
200 µg Ab; 20 µg BP
$354.00

INSR/Insulin Receptor β 抗体 (CT-3) AC

sc-57342 AC
500 µg/ml, 25% agarose
$416.00

INSR/Insulin Receptor β 抗体 (CT-3) HRP

sc-57342 HRP
200 µg/ml
$316.00

INSR/Insulin Receptor β 抗体 (CT-3) FITC

sc-57342 FITC
200 µg/ml
$330.00

INSR/Insulin Receptor β 抗体 (CT-3) PE

sc-57342 PE
200 µg/ml
$343.00

INSR/Insulin Receptor β 抗体 (CT-3) Alexa Fluor® 488

sc-57342 AF488
200 µg/ml
$357.00

INSR/Insulin Receptor β 抗体 (CT-3) Alexa Fluor® 546

sc-57342 AF546
200 µg/ml
$357.00

INSR/Insulin Receptor β 抗体 (CT-3) Alexa Fluor® 594

sc-57342 AF594
200 µg/ml
$357.00

INSR/Insulin Receptor β 抗体 (CT-3) Alexa Fluor® 647

sc-57342 AF647
200 µg/ml
$357.00

INSR/Insulin Receptor β 抗体 (CT-3) Alexa Fluor® 680

sc-57342 AF680
200 µg/ml
$357.00

INSR/Insulin Receptor β 抗体 (CT-3) Alexa Fluor® 790

sc-57342 AF790
200 µg/ml
$357.00

Does it work for uterine cells? I am standardizing it to a concentration of 1:50, but I can't find anything. I have modified several parameters, but nothing

Asked by: Moni
Thank you for your question. INSR/Insulin Receptor β Anticuerpo (CT-3): sc-57342 is suitable for detection of insulin Rβ of mouse, rat and human origin by WB, IP, IF and IHC(P). You can find our recommended protocols under this link: https://www.scbt.com/resources/protocols
Answered by: Tech Support Europe
Date published: 2024-10-25

Is a blocking pepide available for the sc-57342 Insulin receptor antibody?

Asked by: PaulDA
Thank you for your question. There is no blocking peptide available for this specific antibody.
Answered by: Technical Support
Date published: 2019-09-11

Can you provide information about the hybridoma cell line this antibody was produced from?

Asked by: KMitok
Thank you for your question. The specific clone name for this monoclonal antibody is CT-3. If you require additional information, please contact our Technical Service Department for assistance.
Answered by: Technical Support
Date published: 2019-09-11

I have a clear band of approximately 45 KDa with this antibody. Do you know what it can be? Thank you so much!

Asked by: Jimena
Thank you for your question. We do not have any information regarding a 45 kDa sized band when using this antibody. Please contact our Technical Support Department for help with troubleshooting the antibody, or more detailed information about the expected molecular weight. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-22

In the data sheet , it was written the antigen is C-terminal , does the C-terminal region contain Tryosine domain?

Asked by: TANG QIAN
Thank you for your question. This antibody was made against a recombinant protein fragment including the C-terminal 100 amino acid residues of human insulin receptor (accession#P06213). There are tyrosine domains within the C-terminus but since the antibody has not been epitope mapped, I do not know if the exact epitope includes a Tyrosine. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: asia@scbio.cn or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with insulin Rβ (CT-3): sc-57342 monoclonal antibody?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-10
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Rated 2 out of 5 by from Not well used in immunoblottingWe tested it on HEK293T and dopaminergic neurons lysates. Only in the latter it is slightly visible a band corresponding to mature insulin Rβ chain: 95 kDa. Apparently, no detection of insulin R precursor: 200 kDa. An aspecific band is detected at 70kDa.
Date published: 2024-10-03
Rated 4 out of 5 by from Application of insulin Rβ Antibody for IHC and WBThe insulin Rβ Antibody (CT-3) SC-57342 is suitable for intracellular localization studies of the insulin receptor in 3T3-L1 adipocytes at different stages of differentiation by immunofluorescence and confocal microscopy . The antibody can be also used for Western blot studies of protein extracts from 3T3-L1 adipocytes, including quantitative analyses.
Date published: 2018-09-13
Rated 5 out of 5 by from Works great!Works at 1:50 dilution for immunofluorescence in hES cells
Date published: 2018-03-01
Rated 5 out of 5 by from Works great for IHC in frozen mouse liver sectionsI used this at a 1:250 dilution on frozen mouse liver sections and it worked great! Did not work well in paraffin embedded sections.
Date published: 2018-01-03
Rated 4 out of 5 by from nice bandthe antibody was tested in western blot on rat tissue samples and worked well
Date published: 2017-12-13
Rated 4 out of 5 by from positive result in WBworked well for detection of insulin receptor from cell lysate
Date published: 2017-09-06
Rated 3 out of 5 by from ISR bThis antibody was used for Western blotting of MCF7 and T47D cell lysate。It did not work in T47D。It had some signal with a little background in MCF7.
Date published: 2017-06-15
Rated 4 out of 5 by from relative high sensitivityshow some cross reaction with IGF-1Rb, but generally ok to detect IRb
Date published: 2017-05-17
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INSR/Insulin Receptor β 抗体 (CT-3) is rated 4.2 out of 5 by 19.
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