insulin R beta抗体 (CT-3) 推荐用于 mouse, rat 和 human 来源的insulin Rβ WB, IP, IF 和 IHC(P)检测

insulin Rβ 抗体 (CT-3): sc-57342

insulin Rβ抗体 (CT-3) is rated 4.3 out of 5 by 18.
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说明书

    • 抗-insulin R beta 抗体 CT-3 是小鼠单克隆 IgG1 (kappa轻链),insulin Rβ抗体,规格为200 µg/ml
    • 免疫human来源insulin receptor的C-terminus
    • 抗-insulin R beta 抗体 (CT-3) 推荐用于 WB, IP, IF 和 IHC(P),检测mouse, rat 和human 来源的 insulin Rβ; 不会与 insulin-like growth factor (IGF)receptors产生交联反应
    • 抗-insulin R beta 抗体 (CT-3) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 查看我们强烈推荐的传统抗小鼠IgG二抗的重组替代品 m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP)
    • 关于如何获取insulin Rβ (CT-3): sc-57342免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
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Is a blocking pepide available for the sc-57342 Insulin receptor antibody?

Asked by: PaulDA
Thank you for your question. There is no blocking peptide available for this specific antibody.
Answered by: Technical Support
Date published: 2019-09-11

Can you provide information about the hybridoma cell line this antibody was produced from?

Asked by: KMitok
Thank you for your question. The specific clone name for this monoclonal antibody is CT-3. If you require additional information, please contact our Technical Service Department for assistance.
Answered by: Technical Support
Date published: 2019-09-11

I have a clear band of approximately 45 KDa with this antibody. Do you know what it can be? Thank you so much!

Asked by: Jimena
Thank you for your question. We do not have any information regarding a 45 kDa sized band when using this antibody. Please contact our Technical Support Department for help with troubleshooting the antibody, or more detailed information about the expected molecular weight. Please call us toll-free at (800) 457-3801 option 2, or use the live chat on our website.
Answered by: Technical Support
Date published: 2018-11-22

In the data sheet , it was written the antigen is C-terminal , does the C-terminal region contain Tryosine domain?

Asked by: TANG QIAN
Thank you for your question. This antibody was made against a recombinant protein fragment including the C-terminal 100 amino acid residues of human insulin receptor (accession#P06213). There are tyrosine domains within the C-terminus but since the antibody has not been epitope mapped, I do not know if the exact epitope includes a Tyrosine. If you have any further questions, please contact our Asia Technical Service team. You can reach them by phone at (86 21) 6093-6350, by email at: [email protected] or by live chat directly on our website, www.scbt.com
Answered by: Tech Service
Date published: 2017-09-05

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with insulin Rβ (CT-3): sc-57342 monoclonal antibody?

Asked by: jenni
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 4
Date published: 2017-01-10
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Rated 4 out of 5 by from Application of insulin Rβ Antibody for IHC and WB The insulin Rβ Antibody (CT-3) SC-57342 is suitable for intracellular localization studies of the insulin receptor in 3T3-L1 adipocytes at different stages of differentiation by immunofluorescence and confocal microscopy . The antibody can be also used for Western blot studies of protein extracts from 3T3-L1 adipocytes, including quantitative analyses.
Date published: 2018-09-13
Rated 5 out of 5 by from Works great! Works at 1:50 dilution for immunofluorescence in hES cells
Date published: 2018-03-01
Rated 5 out of 5 by from Works great for IHC in frozen mouse liver sections I used this at a 1:250 dilution on frozen mouse liver sections and it worked great! Did not work well in paraffin embedded sections.
Date published: 2018-01-03
Rated 4 out of 5 by from nice band the antibody was tested in western blot on rat tissue samples and worked well
Date published: 2017-12-13
Rated 4 out of 5 by from positive result in WB worked well for detection of insulin receptor from cell lysate
Date published: 2017-09-06
Rated 3 out of 5 by from ISR b This antibody was used for Western blotting of MCF7 and T47D cell lysate。It did not work in T47D。It had some signal with a little background in MCF7.
Date published: 2017-06-15
Rated 4 out of 5 by from relative high sensitivity show some cross reaction with IGF-1Rb, but generally ok to detect IRb
Date published: 2017-05-17
Rated 1 out of 5 by from Confronto tra anticorpo poli e monoclonale A parità di proteine caricate (40ug) e condizioni di diluizione anticorpo (1:1000 in milk 2.5%) il nuovo anticorpo monoclonale (sc-57342) non funziona in modo soddisfacente rispetto al vecchio anticorpo policlonale (sc-711).
Date published: 2017-04-03
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