IKK beta Antibody (G-8): sc-271782
- IKKβ Antibody (G-8) is a mouse monoclonal IgG2a κ, cited in 10 publications, provided at 200 µg/ml
- specific for an epitope mapping between amino acids 707-736 at the C-terminus of IKKβ of human origin
- recommended for detection of IKKβ of human origin by WB, IP, IF, IHC(P) and ELISA
- See IKKβ (H-4): sc-8014 for IKKβ antibody conjugates, including AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 and 790.
- m-IgG Fc BP-HRP and m-IgG2a BP-HRP are the preferred secondary detection reagents for IKKβ Antibody (G-8) for WB and IHC(P) applications. These reagents are now offered in bundles with IKKβ Antibody (G-8) (see ordering information below).
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IKKβ Antibody (G-8) is a mouse monoclonal IgG2a antibody that detects IKKβ in human samples through various applications including western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA). IKKβ, a crucial component of the IκB kinase complex, plays a vital role in activating the transcription factor NFκB, which regulates immune responses, inflammation, and cell survival. IKKβ is primarily located in the cytoplasm, where IKKβ interacts with IκB proteins to facilitate their phosphorylation and subsequent degradation, allowing NFκB to translocate to the nucleus and initiate gene transcription. This localization ensures NFκB remains inactive until needed, preventing inappropriate activation that could lead to chronic inflammation or autoimmune diseases. The IKK complex, which includes IKKα, IKKβ, and IKKγ (NEMO), is essential for phosphorylating IκB proteins, and precise regulation of this process maintains cellular homeostasis and responds to extracellular signals. Anti-IKKβ antibody (G-8) serves as an invaluable tool for researchers studying the NFκB signaling pathway and its implications in various diseases, including cancer and inflammatory disorders.
Alexa Fluor® is a trademark of Molecular Probes Inc., OR., USA
LI-COR® and Odyssey® are registered trademarks of LI-COR Biosciences
IKK beta Antibody (G-8) References:
- Targeted deletion of hepatocyte Ikkbeta confers growth advantages. | Koch, KS., et al. 2009. Biochem Biophys Res Commun. 380: 349-54. PMID: 19171122
- Ectopic expression of CD74 in Ikkβ-deleted mouse hepatocytes. | Koch, KS. and Leffert, HL. 2011. Acta Histochem. 113: 428-35. PMID: 20569972
- IL-1β-driven neutrophilia preserves antibacterial defense in the absence of the kinase IKKβ. | Hsu, LC., et al. 2011. Nat Immunol. 12: 144-50. PMID: 21170027
- IKKβ regulates essential functions of the vascular endothelium through kinase-dependent and -independent pathways. | Ashida, N., et al. 2011. Nat Commun. 2: 318. PMID: 21587235
- Ubiquitin-like domain of IKKβ regulates osteoclastogenesis and osteolysis. | Zhang, Y., et al. 2013. Calcif Tissue Int. 93: 78-85. PMID: 23686246
- Nuclear Receptor PPARα Agonist Wy-14,643 Ameliorates Hepatic Cell Death in Hepatic IKKβ-Deficient Mice. | Kim, T., et al. 2017. Biomol Ther (Seoul). 25: 504-510. PMID: 28190320
- IKKβ inhibition prevents fat-induced beta cell dysfunction in vitro and in vivo in rodents. | Ivovic, A., et al. 2017. Diabetologia. 60: 2021-2032. PMID: 28725915
- Xanthohumol prevents dextran sulfate sodium-induced colitis via inhibition of IKKβ/NF-κB signaling in mice. | Cho, JM., et al. 2018. Oncotarget. 9: 866-880. PMID: 29416662
- IKKβ activates p53 to promote cancer cell adaptation to glutamine deprivation. | Ishak Gabra, MB., et al. 2018. Oncogenesis. 7: 93. PMID: 30478303
- Endogenous hydrogen sulfide sulfhydrates IKKβ at cysteine 179 to control pulmonary artery endothelial cell inflammation. | Zhang, D., et al. 2019. Clin Sci (Lond). 133: 2045-2059. PMID: 31654061
Ordering Information
| Product Name | Catalog # | UNIT | Price | Qty | FAVORITES | |
IKKβ Antibody (G-8) | sc-271782 | 200 µg/ml | $322.00 | |||
IKKβ Antibody (G-8): m-IgG Fc BP-HRP Bundle | sc-540264 | 200 µg Ab; 10 µg BP | $361.00 | |||
IKKβ Antibody (G-8): m-IgG2a BP-HRP Bundle | sc-546878 | 200 µg Ab; 10 µg BP | $361.00 | |||
IKKβ (G-8) Neutralizing Peptide | sc-271782 P | 100 µg/0.5 ml | $69.00 |