Date published: 2025-9-6
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I2PP2A Antibody (F-9) - Western Blotting - Image 136095
I2PP2A 抗体 (F-9): sc-133138. NTERA-2 cl. D1 (A), HeLa (B) 和 F9 (C) 全细胞裂解液中 I2PP2A 表达的 Western 印迹分析.
I2PP2A 抗体 (F-9): sc-133138. NTERA-2 cl. D1 (A), HeLa (B) 和 F9 (C) 全细胞裂解液中 I2PP2A 表达的 Western 印迹分析.

I2PP2A 抗体 (F-9): sc-133138

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说明书
  • I2PP2A 抗体 F-9 是小鼠单克隆 IgG2a κ，I2PP2A抗体, 在19篇文献中引用，规格为200 µg/ml
  • 免疫human物种的I2PP2A的氨基酸1-120
  • I2PP2A 抗体 (F-9) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 I2PP2A
  • 抗I2PP2A抗体（F-9）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取I2PP2A (F-9): sc-133138的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 I2PP2A 抗体 (F-9) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与I2PP2A 抗体 (F-9) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
蛋白序列
说明书与实验方案
研究信息

関連項目

I2PP2A抗体（F-9）是一种IgG2a κ小鼠单克隆I2PP2A抗体（也称为HLA-DR相关蛋白II抗体、PHAPII抗体、磷酸酶2A抑制剂IPP2A2抗体、模板激活因子-I抗体、TAF-IBETA抗体、颗粒酶A激活的DNase抑制剂抗体、IGAAD抗体或核小体乙酰化抑制复合物组分抗体），可通过蛋白质印迹、免疫沉淀、免疫荧光、免疫组化和酶联免疫吸附试验检测小鼠、大鼠和人源的I2PP2A蛋白。I2PP2A抗体（F-9）既有非共轭形式的抗I2PP2A抗体，也有多种共轭形式的抗I2PP2A抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®共轭物。蛋白质磷酸酶2A（PP2A）是一种主要的哺乳动物蛋白质丝氨酸/苏氨酸磷酸酶，可调节多种细胞过程。PP2A的抑制剂1（I1PP2A）和抑制剂2（I2PP2A）在序列上具有很大的相似性，是PP2A细胞磷酸酶活性的热稳定性蛋白质抑制剂。I1PP2A和I2PP2A最初被鉴定为假定的HLA II类相关蛋白Phap I和Phap II。这些抑制蛋白以非竞争性方式选择性抑制PP2A，但不影响相关蛋白PP1、PP2B和PP2C的磷酸酶活性。I1PP2A蛋白定位于细胞质和细胞核。相比之下，I2PP2A主要位于细胞核内，在Wilms肿瘤细胞中高表达。在HEK-293细胞中瞬时表达I2PP2A会导致原癌基因c-Jun的DNA结合活性增加。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

I2PP2A 抗体 (F-9) 参考文献:

  1. 蛋白磷酸酶 2A 抑制剂 I2PP2A 的表达可诱导 c-Jun 和 AP-1 的活性。  |  Al-Murrani, SW., et al. 1999. Biochem J. 341 (Pt 2): 293-8. PMID: 10393085
  2. I2PP2A 参与了 tau 的异常过度磷酸化并被 Memantine 逆转。  |  Chohan, MO., et al. 2006. FEBS Lett. 580: 3973-9. PMID: 16806196
  3. SET/I2PP2A 对妇科癌症的致癌作用  |  Jiang, SW., et al. 2017. Curr Drug Targets. 18: 1152-1157. PMID: 28356029
  4. SET/I2PP2A 蛋白在胃肠道组织中的分布。  |  Umata, K., et al. 2019. PLoS One. 14: e0222845. PMID: 31557212
  5. SET/TAF1β/I2PP2A 三十年：从生物功能的鉴定到其在癌症和阿尔茨海默病中的意义。  |  Di Mambro, A. and Esposito, MT. 2022. Biosci Rep. 42: PMID: 36345878
  6. 自磷酸化激活的蛋白激酶可使蛋白磷酸酶 2A 的蛋白酪氨酸磷酸酶活性失活。  |  Damuni, Z., et al. 1994. FEBS Lett. 352: 311-4. PMID: 7523189
  7. 从牛肾中纯化两种强效热稳定蛋白磷酸酶 2A 抑制剂并确定其特性。  |  Li, M., et al. 1995. Biochemistry. 34: 1988-96. PMID: 7531497
  8. 自磷酸化激活的蛋白激酶使蛋白磷酸酶 2A 磷酸化并失活。  |  Guo, H. and Damuni, Z. 1993. Proc Natl Acad Sci U S A. 90: 2500-4. PMID: 7681598
  9. 髓性白血病相关蛋白 SET 是蛋白磷酸酶 2A 的强效抑制剂。  |  Li, M., et al. 1996. J Biol Chem. 271: 11059-62. PMID: 8626647
  10. 蛋白磷酸酶 2A 的新型强效热稳定阻抑剂蛋白 I1PP2A 的分子鉴定。  |  Li, M., et al. 1996. Biochemistry. 35: 6998-7002. PMID: 8679524
  11. I1PP2A 和 I2PP2A。两种强效的蛋白磷酸酶 2A 特异性抑制蛋白。  |  Li, M. and Damuni, Z. 1998. Methods Mol Biol. 93: 59-66. PMID: 9664527
  12. 蛋白磷酸酶 2A 抑制剂 SET 在肾脏发育和威尔瘤中的表达。  |  Carlson, SG., et al. 1998. J Am Soc Nephrol. 9: 1873-80. PMID: 9773788

订购信息

产品名称产品编号规格价格数量收藏夹

I2PP2A 抗体 (F-9)

sc-133138
200 µg/ml
$316.00

I2PP2A (F-9): m-IgGκ BP-HRP 套装

sc-521248
200 µg Ab, 40 µg BP
$354.00

I2PP2A (F-9): m-IgG2a BP-HRP 套装

sc-546092
200 µg Ab; 10 µg BP
$354.00

I2PP2A 抗体 (F-9) AC

sc-133138 AC
500 µg/ml, 25% agarose
$416.00

I2PP2A 抗体 (F-9) HRP

sc-133138 HRP
200 µg/ml
$316.00

I2PP2A 抗体 (F-9) FITC

sc-133138 FITC
200 µg/ml
$330.00

I2PP2A 抗体 (F-9) PE

sc-133138 PE
200 µg/ml
$343.00

I2PP2A 抗体 (F-9) Alexa Fluor® 488

sc-133138 AF488
200 µg/ml
$357.00

I2PP2A 抗体 (F-9) Alexa Fluor® 546

sc-133138 AF546
200 µg/ml
$357.00

I2PP2A 抗体 (F-9) Alexa Fluor® 594

sc-133138 AF594
200 µg/ml
$357.00

I2PP2A 抗体 (F-9) Alexa Fluor® 647

sc-133138 AF647
200 µg/ml
$357.00

I2PP2A 抗体 (F-9) Alexa Fluor® 680

sc-133138 AF680
200 µg/ml
$357.00

I2PP2A 抗体 (F-9) Alexa Fluor® 790

sc-133138 AF790
200 µg/ml
$357.00

記載された情報によると、この抗体の認識するI2PP2Aのたんぱく質配列はアイソフォーム2(uniprot参照)に相当する配列かと思いますが、エピトープは1-120アミノ酸残基ということを踏まえると、I2PP2Aアイソフォーム1(アイソフォーム2とは1-37アミノ酸残基が異なる)は認識できないということでよろしいですか？また、アイソフォーム1を認識できる抗体の販売は行なっていますか？

Asked by: Fujita
Thanks for your question.I2PP2A Antibody (F-9): sc-133138 is raised against amino acids 1-120 of I2PP2A of human origin.Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer if this primary antibody can reactive with your target protein . Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website.
Answered by: Technical Support 9
Date published: 2020-07-10

Can this antibody be used to detect I2PP2A of bovine origin?

Asked by: AbPolly
Thank you for your question. Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 2
Date published: 2017-01-24
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Rated 4 out of 5 by from good productWe got clean band for WB experiment with some background.
Date published: 2018-09-11
Rated 3 out of 5 by from Nice antibody for WB!This antibody works fine to detect I2PP2A in WB in human samples. In my hands it did not work in IF.
Date published: 2018-04-03
Rated 5 out of 5 by from Specific band in Western BlotI got the antibody as a free sample to test for Western Blot. It arrived very quickly and it works really nice for Western Blot. I used it in a 1:500 dilution (recommended was to start with a 1:100 dilution).
Date published: 2018-02-02
Rated 5 out of 5 by from Excellent antibodyI received this antibody as a sample and used it to probe rat astrocyte lysates. I worked just as predicted. Blots were very clean when I used the IgG kappa chain secondary. See image.
Date published: 2017-10-05
Rated 5 out of 5 by from western blotI used this antibody for western blot in a dilution of 1:2000 with great results. The signal was very strong, however. No nonspecific bands were detected.
Date published: 2017-08-19
Rated 5 out of 5 by from Robust signal for WBI'm glad to use it and get good results with it in WB.
Date published: 2017-05-31
Rated 5 out of 5 by from Positive resultPositive WB data in DU 145 whole cell lysate,strong band seen.
Date published: 2017-05-25
Rated 5 out of 5 by from worked niceIt worked nice when I used for WB of mouse liver tissue,I use the dilution 1:500.
Date published: 2017-05-13
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I2PP2A 抗体 (F-9) is rated 4.7 out of 5 by 15.
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