Date published: 2025-9-7
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HSP 47 Antibody (G-12) - Western Blotting - Image 356340
HSP 47 抗体 (G-12): sc-5293. MCF7 (A), SK-N-SH (B), Sol8 (C), LADMAC (D), L6 (E) 和 A-10 (F) 全细胞裂解液中 HSP 47 表达的 Western 印迹分析.
HSP 47 抗体 (G-12): sc-5293. MCF7 (A), SK-N-SH (B), Sol8 (C), LADMAC (D), L6 (E) 和 A-10 (F) 全细胞裂解液中 HSP 47 表达的 Western 印迹分析.

HSP 47 抗体 (G-12): sc-5293

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说明书
  • HSP 47 抗体 G-12 是小鼠单克隆 IgG2a κ，HSP 47抗体, 在22篇文献中引用，规格为200 µg/ml
  • 免疫human物种的HSP 47的氨基酸129-300
  • HSP 47 抗体 (G-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 HSP 47
  • 抗HSP 47抗体（G-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSP 47 (G-12): sc-5293的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgGκ BP-HRP 是 HSP 47 抗体 (G-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。该试剂现与HSP 47 抗体 (G-12) 组合成套装提供。 (请参阅下面的订购信息)。其他m-IgGκ BP 偶联产品，请参阅我们的小鼠 IgG 结合蛋白完整列表。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 HSP 抗体 包括 HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60和HSP 65

HSP 47 抗体（G-12）是一种小鼠单克隆 IgG2a kappa 轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的 HSP 47 蛋白。抗HSP 47抗体（G-12）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。HSP 47，也称为胶原蛋白1、血清素h1和胶原蛋白结合蛋白1（CBP1），主要位于产生前胶原细胞的细胞内质网中，在胶原蛋白的正常折叠和组装中发挥着关键作用。HSP 47对于I型胶原蛋白的生物合成至关重要，而I型胶原蛋白可维持皮肤、骨骼和软骨等各种组织的结构完整性。HSP 47通过分子伴侣活动，确保细胞分泌前正确折叠前胶原，防止错误折叠的蛋白质堆积，从而避免细胞压力和组织损伤。HSP 47的合成会因转化生长因子β（TGFβ）和白细胞介素-1β（IL-1β）而增加，这凸显了HSP 47在细胞应激反应和组织重塑中的作用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSP 47 抗体 (G-12) 参考文献:

  1. Hsp47（Serpin H1）生物学，一种胶原蛋白特异性分子伴侣。  |  Ito, S. and Nagata, K. 2017. Semin Cell Dev Biol. 62: 142-151. PMID: 27838364
  2. 哺乳动物高尔基应激反应的TFE3、HSP47和CREB3通路。  |  Taniguchi, M. and Yoshida, H. 2017. Cell Struct Funct. 42: 27-36. PMID: 28179603
  3. 伴侣蛋白HSP47：一种有助于血栓形成和止血的血小板胶原蛋白结合蛋白。  |  Sasikumar, P., et al. 2018. J Thromb Haemost. 16: 946-959. PMID: 29512284
  4. 内质网驻留的胶原蛋白特异性分子伴侣Hsp47在脊椎动物细胞和人类疾病中的作用。  |  Ito, S. and Nagata, K. 2019. J Biol Chem. 294: 2133-2141. PMID: 30541925
  5. Hsp47通过增强胶原依赖性癌细胞-血小板相互作用促进癌症转移。  |  Xiong, G., et al. 2020. Proc Natl Acad Sci U S A. 117: 3748-3758. PMID: 32015106
  6. 调节HSP47在巩膜成纤维细胞中胶原代谢的表达效果。  |  Guo, Q., et al. 2021. Curr Eye Res. 46: 408-416. PMID: 32814454
  7. HSP47通过诱导膀胱癌中的CCL2促进血管生成。  |  Ma, W., et al. 2021. Cell Signal. 85: 110044. PMID: 34000383
  8. 靶向HSP47和HSP70：肝纤维化管理的有前景的治疗方案。  |  Abd El-Fattah, EE. and Zakaria, AY. 2022. J Transl Med. 20: 544. PMID: 36435779
  9. HSP47通过IRE1α激活、XBP1剪接和β-Catenin核易位增加成纤维细胞中I型胶原蛋白的表达。  |  Ham, SY., et al. 2024. Cells. 13: PMID: 38534372
  10. Hsp47促进内质网中多亚基神经受体的生物合成。  |  Wang, YJ., et al. 2024. Elife. 13: PMID: 38963323

订购信息

产品名称产品编号规格价格数量收藏夹

HSP 47 抗体 (G-12)

sc-5293
200 µg/ml
$316.00

HSP 47 (G-12): m-IgGκ BP-HRP 套装

sc-520457
200 µg Ab, 40 µg BP
$354.00

HSP 47 抗体 (G-12) AC

sc-5293 AC
500 µg/ml, 25% agarose
$416.00

HSP 47 抗体 (G-12) HRP

sc-5293 HRP
200 µg/ml
$316.00

HSP 47 抗体 (G-12) FITC

sc-5293 FITC
200 µg/ml
$330.00

HSP 47 抗体 (G-12) PE

sc-5293 PE
200 µg/ml
$343.00

HSP 47 抗体 (G-12) Alexa Fluor® 488

sc-5293 AF488
200 µg/ml
$357.00

HSP 47 抗体 (G-12) Alexa Fluor® 546

sc-5293 AF546
200 µg/ml
$357.00

HSP 47 抗体 (G-12) Alexa Fluor® 594

sc-5293 AF594
200 µg/ml
$357.00

HSP 47 抗体 (G-12) Alexa Fluor® 647

sc-5293 AF647
200 µg/ml
$357.00

HSP 47 抗体 (G-12) Alexa Fluor® 680

sc-5293 AF680
200 µg/ml
$357.00

HSP 47 抗体 (G-12) Alexa Fluor® 790

sc-5293 AF790
200 µg/ml
$357.00

Do you have the blocking peptide for HSP 47 Antibody (G-12): sc-5293?

Asked by: Manjunath Nayak
Thank you for the question. This antibody was raised against amino acids 129-300 of HSP 47 of human origin. A blocking peptide / recomb. protein fragment with a length of >170 aas would commonly be too long to efficiently block the antibody binding sites of this monoclonal antibody in a competition assay. Unfortunately, we therefore do not offer the corresponding blocking / negative control reagent. Instead, you might want to try a -NIC or CRISPR or siRNA negative control in case you are working with cultured cells.
Answered by: Technical Support Europe
Date published: 2018-01-31

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Thank you for your question Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 5 out of 5 by from HSP47 is a good antibody for WBI used this antibody with mouse bone samples and gave me clean and strong bands. Primary antibody dilution 1:500. Secondary antibody m-IgGκ BP-HRP dilution 1:2000.
Date published: 2022-06-03
Rated 5 out of 5 by from Perfect antibody against Hsp47Hsp47 is collagen-specific ER chaperone. We used this antibodies for multiple applications including western blotting, immunocytostaining and immunofluorescence of tissues (paraffin). All staining was specific and strong. Specificity of this antibody was validated using inhibitor of up-stream molecule (site-1 protease). I strongly suggest to purchase this clone from Santa Cruz.
Date published: 2019-08-28
Rated 5 out of 5 by from excellentウエスタンブロットおよび蛍光免疫染色に使用。ノンスペバンドもなく、また低濃度でも十分に検出できます。
Date published: 2019-04-24
Rated 5 out of 5 by from Antibodies nicely working for WBBand of appropriate size after immunobloting of LN229 cell lystes.
Date published: 2018-02-16
Rated 5 out of 5 by from Good antibodyThis antibody shows great reactivity. It is highly specific
Date published: 2017-09-19
Rated 5 out of 5 by from Western BlotWe used the HSP 47 antibody for Western Blot, the stripes are very fine at 47kda.
Date published: 2017-05-31
Rated 5 out of 5 by from strong recommend antibodyWe observed clear band when diluted 1:500 ，Great help to us, strongly recommended.
Date published: 2017-05-28
Rated 5 out of 5 by from works very goodWe use it usually , works very well in WB at 1:500 dilution.
Date published: 2017-02-26
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HSP 47 抗体 (G-12) is rated 4.9 out of 5 by 14.
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