HSP 47抗体 (G-12) 推荐用于 mouse, rat 和 human 来源的HSP 47 WB, IP, IF, IHC(P) 和 ELISA检测

HSP 47 抗体 (G-12): sc-5293

HSP 47抗体 (G-12) is rated 4.9 out of 5 by 13.
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说明书

    • 抗-HSP 47 抗体 G-12 是小鼠单克隆 IgG2a κ,HSP 47抗体, 在14篇文献中引用,规格为200 µg/ml
    • 免疫human物种的HSP 47的氨基酸129-300
    • 抗-HSP 47 抗体 (G-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 HSP 47
    • 抗-HSP 47 抗体 (G-12) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取HSP 47 (G-12): sc-5293免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred detection reagent for HSP 47 Antibody (G-12) for WB and IHC(P) applications. This reagent is now offered in a bundle with HSP 47 Antibody (G-12) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

Do you have the blocking peptide for HSP 47 Antibody (G-12): sc-5293?

Asked by: Manjunath Nayak
Thank you for the question. This antibody was raised against amino acids 129-300 of HSP 47 of human origin. A blocking peptide / recomb. protein fragment with a length of >170 aas would commonly be too long to efficiently block the antibody binding sites of this monoclonal antibody in a competition assay. Unfortunately, we therefore do not offer the corresponding blocking / negative control reagent. Instead, you might want to try a -NIC or CRISPR or siRNA negative control in case you are working with cultured cells.
Answered by: Technical Support Europe
Date published: 2018-01-31

I need to use an antibody to detect the protein in samples of canine origin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Thank you for your question Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Santa Cruz TS
Date published: 2017-01-27
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Rated 5 out of 5 by from Perfect antibody against Hsp47 Hsp47 is collagen-specific ER chaperone. We used this antibodies for multiple applications including western blotting, immunocytostaining and immunofluorescence of tissues (paraffin). All staining was specific and strong. Specificity of this antibody was validated using inhibitor of up-stream molecule (site-1 protease). I strongly suggest to purchase this clone from Santa Cruz.
Date published: 2019-08-28
Rated 5 out of 5 by from excellent ウエスタンブロットおよび蛍光免疫染色に使用。ノンスペバンドもなく、また低濃度でも十分に検出できます。
Date published: 2019-04-24
Rated 5 out of 5 by from Antibodies nicely working for WB Band of appropriate size after immunobloting of LN229 cell lystes.
Date published: 2018-02-16
Rated 5 out of 5 by from Good antibody This antibody shows great reactivity. It is highly specific
Date published: 2017-09-19
Rated 5 out of 5 by from Western Blot We used the HSP 47 antibody for Western Blot, the stripes are very fine at 47kda.
Date published: 2017-05-31
Rated 5 out of 5 by from strong recommend antibody We observed clear band when diluted 1:500 ,Great help to us, strongly recommended.
Date published: 2017-05-28
Rated 5 out of 5 by from works very good We use it usually , works very well in WB at 1:500 dilution.
Date published: 2017-02-26
Rated 5 out of 5 by from A strong HSP 47 monoclonal choice! This excellent antibody has great reactivity in a wide range of applications. It demonstrates strong bands in a Western blot and has provide nice immunofluoresence and Immunoperoxidase staining . This product is well characterized in the primary literature.
Date published: 2016-12-20
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