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HSP 40 Antibody (B-3) - Western Blotting - Image 308385
HSP 40 抗体 (B-3): sc-398766. Jurkat (A) 和 K-562 (B) 全细胞裂解液中 HSP 40 表达的 Western 印迹分析.
HSP 40 Antibody (B-3) - Western Blotting - Image 308385
HSP 40 抗体 (B-3): sc-398766. Jurkat (A) 和 K-562 (B) 全细胞裂解液中 HSP 40 表达的 Western 印迹分析.
HSP 40 Antibody (B-3) - Western Blotting - Image 362325
HSP 40 抗体 (B-3): sc-398766. Hep G2 (A), SW480 (B), MCF7 (C), F9 (D) 和 Sol8 (E) 全细胞裂解液及大鼠睾丸组织提取物 (F) 中 HSP 40 表达的 Western 印迹分析.
HSP 40 抗体 (B-3): sc-398766. Jurkat (A) 和 K-562 (B) 全细胞裂解液中 HSP 40 表达的 Western 印迹分析.

HSP 40 抗体 (B-3): sc-398766

4.8(8)
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说明书
  • HSP 40 抗体 B-3 是小鼠单克隆 IgG1 κ，HSP 40抗体, 在8篇文献中引用，规格为200 µg/ml
  • 免疫原氨基酸序列241-340位于human物种的HSP 40 protein 1的C-terminus
  • HSP 40 抗体 (B-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 HSP 40 subfamily B member 1; 也和以下物种反应，包括： 和 canine and bovine
  • 抗HSP 40抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取HSP 40 (B-3): sc-398766的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是HSP 40 Antibody (B-3) for WB applications. 的首选辅助检测试剂，这些试剂现与HSP 40 Antibody (B-3) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

额外HSP抗体，包括HSC 70, HSP 10, HSP 105, HSP 20, HSP 27, HSP 40, HSP 47, HSP 56, HSP 60, HSP 65, HSP 70, HSP 75, HSP 90, HSP 90α, HSP 90β和HSP 40-4

HSP 40抗体（B-3）是一种IgG1 κ小鼠单克隆HSP 40抗体（也称为HSP 40抗体），可通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人类的HSP 40蛋白。HSP 40抗体（B-3）既有非偶联形式的抗HSP 40抗体，也有多种偶联形式的抗HSP 40抗体，包括琼脂糖、HRP、PE、FITC和多种Alexa Fluor®偶联物。热休克蛋白40（HSP 40）家族蛋白通过其J结构域与HSP 70结合，并通过刺激HSP 70 ATPase活性来调节HSP 70的功能。HSP 40，也被称为DnaJ，与DnaK（HSP 70）和GrpE一起作为分子伴侣起作用，参与蛋白质复合物的组装和拆卸、蛋白质折叠、变性蛋白质的复性、防止蛋白质聚集和蛋白质输出。HSP 40刺激HSC 70和HIP之间的关联，并在热休克时从细胞质迅速转移到细胞核，特别是核仁。目前已知有五种HSP 40家族蛋白。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

HSP 40 抗体 (B-3) 参考文献:

  1. 热休克蛋白（Hsp）40 突变体抑制哺乳动物细胞中的 Hsp70。  |  Michels, AA., et al. 1999. J Biol Chem. 274: 36757-63. PMID: 10593983
  2. 哺乳动物 HSP40/DNAJ 同源物：新型 cDNA 的克隆及其分类和命名建议。  |  Ohtsuka, K. and Hata, M. 2000. Cell Stress Chaperones. 5: 98-112. PMID: 11147971
  3. 第二类 Hsp40 Sis1 在维持酵母朊病毒中的特异性 [RNQ+]。  |  Lopez, N., et al. 2003. Mol Biol Cell. 14: 1172-81. PMID: 12631732
  4. 热休克蛋白（Hsp）70 和 Hsp 40 在结直肠癌中的表达。  |  Kanazawa, Y., et al. 2003. Med Oncol. 20: 157-64. PMID: 12835518
  5. HSP40/DNAJ 在调节 HIV-1 复制中的新作用。  |  Urano, E., et al. 2013. J Acquir Immune Defic Syndr. 64: 154-62. PMID: 24047968
  6. 内质网 HSP40 辅伴侣蛋白 ERdj3/DNAJB11 以四聚物的形式组装并发挥作用。  |  Chen, KC., et al. 2017. EMBO J. 36: 2296-2309. PMID: 28655754
  7. 将 Hsp40/Hsp70 伴侣轴作为治疗阉割耐药前列腺癌的新策略。  |  Moses, MA., et al. 2018. Cancer Res. 78: 4022-4035. PMID: 29764864
  8. 哺乳动物细胞中 hsp40（DnaJ）与 hsp70（DnaK）的同源定位和共定位。  |  Yamane, M., et al. 1995. Cell Struct Funct. 20: 157-66. PMID: 7641298
  9. 热休克蛋白 40--小鼠细胞耐热性的新型预测因子  |  Kaneko, R., et al. 1995. Radiat Res. 142: 91-7. PMID: 7899564
  10. ATP 对热休克 HeLa 细胞核中 hsp 70 和 hsp 40 释放的影响。  |  Ohtsuka, K., et al. 1993. Exp Cell Res. 209: 357-66. PMID: 8262154
  11. 从人类肝脏中分离出 DnaJ 样热休克蛋白 40 (Hsp40) 的新成员。  |  Hoe, KL., et al. 1998. Biochim Biophys Acta. 1383: 4-8. PMID: 9546042
  12. 大鼠大脑突触后结构中存在分子伴侣--热休克同源物（Hsc）70和热休克蛋白（Hsp）40。  |  Suzuki, T., et al. 1999. Brain Res. 816: 99-110. PMID: 9878698

订购信息

产品名称产品编号规格价格数量收藏夹

HSP 40 抗体 (B-3)

sc-398766
200 µg/ml
$316.00

HSP 40 (B-3): m-IgG Fc BP-HRP 套装

sc-527809
200 µg Ab; 10 µg BP
$354.00

HSP 40 (B-3): m-IgG1 BP-HRP 套装

sc-533182
200 µg Ab; 20 µg BP
$354.00

HSP 40 抗体 (B-3) AC

sc-398766 AC
500 µg/ml, 25% agarose
$416.00

HSP 40 抗体 (B-3) HRP

sc-398766 HRP
200 µg/ml
$316.00

HSP 40 抗体 (B-3) FITC

sc-398766 FITC
200 µg/ml
$330.00

HSP 40 抗体 (B-3) PE

sc-398766 PE
200 µg/ml
$343.00

HSP 40 抗体 (B-3) Alexa Fluor® 488

sc-398766 AF488
200 µg/ml
$357.00

HSP 40 抗体 (B-3) Alexa Fluor® 546

sc-398766 AF546
200 µg/ml
$357.00

HSP 40 抗体 (B-3) Alexa Fluor® 594

sc-398766 AF594
200 µg/ml
$357.00

HSP 40 抗体 (B-3) Alexa Fluor® 647

sc-398766 AF647
200 µg/ml
$357.00

HSP 40 抗体 (B-3) Alexa Fluor® 680

sc-398766 AF680
200 µg/ml
$357.00

HSP 40 抗体 (B-3) Alexa Fluor® 790

sc-398766 AF790
200 µg/ml
$357.00

What is the recommended starting dilution for Western blot for sc-398766: HSP 40 (B-3) monoclonal antibody?

Asked by: cjMara
The recommended starting dilution for Western blot is 1:100 with a dilution range of 1:100-1:1000. This information, along with other technical specifications, is available in the recommended protocol: https://www.scbt.com/scbt/resources/protocols/western-immuno-blotting
Answered by: Technical Support
Date published: 2017-03-22
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Rated 4 out of 5 by from Gute Ergebnisse im Western Blot.Verdünnungen von 1:500 und 1:1000 zeigen beide gute Banden und kein Hintergrundsignal! Wir sind zufrieden!
Date published: 2021-03-22
Rated 5 out of 5 by from Very good antibody for WBVery good and specific band detection at diluition 1:1000 in western blotting of human bronchial cell lines 16-HBE
Date published: 2018-10-05
Rated 5 out of 5 by from Great antibody!1:500 diluted for wb of rat protein,the band is strong and special
Date published: 2018-01-31
Rated 5 out of 5 by from Works well for WBworks well for western - samples were human cells in culture
Date published: 2017-11-21
Rated 5 out of 5 by from great antibody for western blotI recently used it for western blot at 1/1000 dilution, and it gives very good bands.
Date published: 2017-08-29
Rated 5 out of 5 by from Great for WBThe HSP 40 Antibody (B-3): sc-398766 works very well for Western blot applications, showing clear band with minimal background.
Date published: 2017-01-18
Rated 4 out of 5 by from Potent and quite specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was strong detection of the ~40 kDa target protein in an A431/HepG2/MCF7 cell lysate mix, but the target was not detected in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested. A ~25 kDa strongly cross-reactive protein was detectable in the A431/HepG2/MCF7 cell lysate mix.
Date published: 2016-11-10
Rated 5 out of 5 by from Great antibody for WesternGreat antibody for Western, strong band detected in K-562 and Jurkat. -SCBT QC
Date published: 2015-04-06
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HSP 40 抗体 (B-3) is rated 4.8 out of 5 by 8.
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