Hec1抗体 (1) 推荐用于 mouse, rat, human 和 canine 来源的Hec1 WB, IP 和 IF检测

Hec1 抗体 (1): sc-135934

Hec1抗体 (1) is rated 5.0 out of 5 by 3.
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5
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说明书

    • Hec1 抗体 1 是小鼠单克隆 IgG1,提供 50 µg/500 µl
    • 免疫human物种的Hec1的氨基酸495-608
    • 推荐用于 mouse, rat, human 和 canine 来源的Hec1 WB, IP 和 IF检测

I am using Hec1 (1): sc-135934 for immunofluorescence, what dilution of this antibody should I use?

Asked by: AbPolly
Thank you for your question. We recommend a starting dilution of 1:50. However, optimal antibody concentration should be determined by titration. Please find our full immunofluorescence protocol here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-03-24
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Rated 5 out of 5 by from gave a single band Ran cell lysate on a 10 percent gel and transfered to PVDF using 10% Methanol Transfer buffer in a semi dry apparatus. Antibody gave a single band at approximately the expected molecular weight. Hec1 knockdown by siRNA was reflected in the band intensity by western.
Date published: 2017-02-15
Rated 5 out of 5 by from Excellent immunofluorescence cytoplasmic Excellent immunofluorescence cytoplasmic staining in BC3H1 cells. -SCBT QC
Date published: 2015-02-25
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