Date published: 2025-9-6
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DDIT3/GADD153/CHOP Antibody (B-3) - Western Blotting - Image 391469
GADD 153 抗体 (B-3) Alexa Fluor® 488: sc-7351 AF488. 直接荧光西部印迹分析 PC-12 全细胞裂解液中 GADD 153 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.
GADD 153 抗体 (B-3) Alexa Fluor® 488: sc-7351 AF488. 直接荧光西部印迹分析 PC-12 全细胞裂解液中 GADD 153 的表达. 用 UltraCruz® 阻断试剂阻断: sc-516214.

DDIT3/GADD153/CHOP 抗体 (B-3): sc-7351

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寫評論提問

说明书
  • DDIT3/GADD153/CHOP 抗体 B-3 是小鼠单克隆 IgG1 κ，DDIT3/GADD153/CHOP抗体, 在569篇文献中引用，规格为200 µg/ml
  • 免疫1-168氨基酸，对应于mouse来源GADD 153全长
  • DDIT3/GADD153/CHOP 抗体 (B-3) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 GADD 153
  • 抗DDIT3/GADD153/CHOP抗体（B-3）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取DDIT3/GADD153/CHOP (B-3): sc-7351的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成DDIT3/GADD153/CHOP 抗体 (B-3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

快捷链接

相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 GADD 抗体 包括 GADD 153, GADD 34和GADD 45

GADD 153抗体（B-3）是一种IgG1 κ小鼠单克隆GADD 153抗体（也称为CHOP抗体、DDIT-3抗体或CHOP-10抗体），可通过WB、IP、IF和ELISA检测来自小鼠、大鼠和人类的GADD 153蛋白。GADD 153抗体（B-3）既可以是非偶联的抗GADD 153抗体形式，也可以是多种偶联形式的抗GADD 153抗体，包括琼脂、HRP、PE、FITC和多种Alexa Fluor®偶联物。GADD 153被描述为一种生长阻滞和DNA损伤诱导型基因，编码一种与C/EBP相关的核蛋白。该蛋白也被指定为C/EBP同源蛋白（CHOP-10）。GADD 153的表达受到各种细胞应激的诱导，包括营养缺乏和代谢紊乱。GADD 153的功能是阻止细胞在G1到S期的细胞周期进程，并通过与其他C/EBP蛋白二聚化来指导GADD 153二聚体远离"经典"C/EBP结合位点，而是识别出独特的"非经典"位点。因此，GADD 153在某些终末分化细胞中充当负调节剂，类似于Id对Myo D和Myo D相关蛋白活性的调节作用，这些蛋白参与肌肉细胞的发育。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

DDIT3/GADD153/CHOP 抗体 (B-3) 参考文献:

  1. 定量测定GADD153信使RNA水平的变化，作为头颈癌肿瘤反应的分子标记。  |  Los, G., et al. 1999. Clin Cancer Res. 5: 1610-8. PMID: 10430059
  2. 热诱导启动子 gadd 153 下的旁观者杀伤效应和 TNF-α 基因的周期性诱导。  |  Ito, A., et al. 2000. J Biosci Bioeng. 90: 437-41. PMID: 16232886
  3. G1 阶段的进展：根据提示循环。  |  Sherr, CJ. 1994. Cell. 79: 551-5. PMID: 7954821
  4. 细胞周期蛋白与癌症。二：细胞周期蛋白 D 和 CDK 抑制剂的时代到来。  |  Hunter, T. and Pines, J. 1994. Cell. 79: 573-82. PMID: 7954824
  5. p53 调控蛋白 Gadd45 与增殖细胞核抗原的相互作用。  |  Smith, ML., et al. 1994. Science. 266: 1376-80. PMID: 7973727
  6. 诱导生长停滞：新的机理认识。  |  Ron, D. 1994. Proc Natl Acad Sci U S A. 91: 1985-6. PMID: 8134335
  7. 新型初级反应基因MyD118和原癌基因myb, myc及bcl-2可调节转化生长因子β1诱导的骨髓性白血病细胞凋亡。  |  Selvakumaran, M., et al. 1994. Mol Cell Biol. 14: 2352-60. PMID: 8139540
  8. gadd 和 MyD 基因定义了一组新的哺乳动物基因，它们编码的酸性蛋白能协同抑制细胞生长。  |  Zhan, Q., et al. 1994. Mol Cell Biol. 14: 2361-71. PMID: 8139541
  9. 紫外线照射对正常人口腔角质细胞和经 HPV 分类的人口腔角质细胞的细胞周期, 活力以及 p53, gadd153 和 gadd45 基因表达的影响。  |  Gujuluva, CN., et al. 1994. Oncogene. 9: 1819-27. PMID: 8208528
  10. 细胞应激过程中 GADD153 与 CCAAT/增强子结合蛋白 beta 之间的物理和功能关联  |  Fawcett, TW., et al. 1996. J Biol Chem. 271: 14285-9. PMID: 8662954
  11. 在用依托泊苷处理的人白血病细胞中，gadd153信使RNA水平的增加与细胞凋亡有关。  |  Eymin, B., et al. 1997. Cancer Res. 57: 686-95. PMID: 9044846
  12. 减数杂交鉴定出一种与生长停滞和 DNA 损伤诱导基因序列同源的转化进展相关基因 PEG-3。  |  Su, ZZ., et al. 1997. Proc Natl Acad Sci U S A. 94: 9125-30. PMID: 9256446

订购信息

产品名称产品编号规格价格数量收藏夹

DDIT3/GADD153/CHOP 抗体 (B-3)

sc-7351
200 µg/ml
$316.00

DDIT3/CHOP/GADD153 抗体 (B-3) AC

sc-7351 AC
500 µg/ml, 25% agarose
$416.00

DDIT3/CHOP/GADD153 抗体 (B-3) HRP

sc-7351 HRP
200 µg/ml
$316.00

DDIT3/CHOP/GADD153 抗体 (B-3) FITC

sc-7351 FITC
200 µg/ml
$330.00

DDIT3/CHOP/GADD153 抗体 (B-3) PE

sc-7351 PE
200 µg/ml
$343.00

DDIT3/CHOP/GADD153 抗体 (B-3) Alexa Fluor® 488

sc-7351 AF488
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (B-3) Alexa Fluor® 546

sc-7351 AF546
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (B-3) Alexa Fluor® 594

sc-7351 AF594
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (B-3) Alexa Fluor® 647

sc-7351 AF647
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (B-3) Alexa Fluor® 680

sc-7351 AF680
200 µg/ml
$357.00

DDIT3/CHOP/GADD153 抗体 (B-3) Alexa Fluor® 790

sc-7351 AF790
200 µg/ml
$357.00

用GADD153（B-3）做不出来核表达，应该怎么办？

Asked by: shanhu
Thank you for your inquiry. Please contact our Technical Service Department. Our Asian Technical Service team is available at (+86) 021-60936350 or asia@scbio.cn or by live chat on our website. 感谢您的提问。请联系我们的技术支持部门。亚洲技术支持电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”。
Answered by: Technical Support 9
Date published: 2019-10-29

请问GADD153（B-3）：7351这个抗体推荐的抗原修复的方法是什么？我做的IHC表达在细胞质，做不出在细胞核中表达的。

Asked by: shanhu
Thank you for your question.Certain antigenic determinants are masked by formalin fixation and paraffin embedding and may be exposed by one of several methods: 1.Heat treatment (recommended method): Place slides in a container and cover with 10 mM sodium citrate buffer, pH 6.0 (sc-294091); or with 50 mM glycine-HCl buffer (sc-29096), pH 3.5, with 0.01% (w/v) EDTA (sc-29092). Heat at 95° C for 5 minutes. Top off with fresh buffer and heat at 95° C for 5 minutes (optimal incubation time may vary for each tissue type). Allow slides to cool in the buffer for approximately 20 minutes. Wash in deionized H2O three times for 2 minutes each. Aspirate excess liquid from slides. 2.Pepsin: Incubate sections for 10–20 minutes in 0.1% Pepsin (sc-476554) in 0.01 N HCl at room temperature. Wash slides several times in deionized H2O. Aspirate excess liquid from slides. 3.Saponin: Incubate sections for 30 minutes in 0.05% Saponin (sc-280079) in deionized H2O at room temperature. Wash at least three times in PBS. Aspirate excess liquid from slides. You can find our recommended protocol here:https://www.scbt.com/zh/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 9
Date published: 2019-10-25

I get a clear and unique band at 45 kDa. Could that be GAD153? It is supposed to dettect a band at 30 kDa.

Asked by: ev0292
Thank you for your question. We are not aware of any literature describing GADD 153 being detected at 45 kDa. Please contact our Technical Service Department for assistance in troubleshooting this antibody.
Answered by: Technical Support
Date published: 2023-02-26

Hi. I´ve been working with this antibody but, somethimes I get a band but other there´s none. I use retinal cells with different treatments. My working dilution goes from 1:500 to 1:1000 on TBS Tween. Can you help me? Thanks

Asked by: Gabsbiochem
Thank you for your question. Please get in touch with our technical service so we can address this issue properly and assist you with troubleshooting your experiments.
Answered by: Tech Support Europe
Date published: 2019-07-05

GADD 153 抗体 (B-3): sc-7351 可以和牛源DDIT3反应吗？以前可以与牛源交叉反应的产品下架了，推荐的产品是该产品。另外该产品针对的抗原表位是哪个物种什么位置？

Asked by: alexws
感谢您的咨询。sc-7351只能用于大鼠 ，小鼠 人样本 It is raised against amino acids 1-168 representing full length GADD 153 of mouse origin。 更多问题，欢迎通过：电话(+86) 021-60936350、邮箱asia@scbio.cn、或选择网页上Live Chat中的“中文支持”，联系我们的技术支持部门。
Answered by: Technical Service 11
Date published: 2018-01-09

What size band does GADD 153 Antibody (B-3): sc-7351 detect for Western blot (WB)?

Asked by: Dr Ninau Qelp
GADD 153 Antibody (B-3): sc-7351 should detect a band at 30 kDa.
Answered by: Tech Service 2
Date published: 2017-08-05

When using GADD 153 Antibody (B-3): sc-7351 for Western blot (WB), what starting dilution do you recommend?

Asked by: Dr Ninau Qelp
For Western blot we recommend a starting dilution of 1:200. And with a dilution range of 1:100-1:1000.
Answered by: Tech Service 2
Date published: 2017-09-08
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Rated 1 out of 5 by from Funzionamento non determinabileAbbiamo usato questo anticorpo su estratti totali di cellule epiteliali SiHa e C4I e non si evidenziano bande dell'altezza attesa
Date published: 2024-10-01
Rated 2 out of 5 by from worked poorlyI tried this antibody in Western blot at 1:500 diluition on HeLa cells exposed to toxic stress (tunicamycin 3mg/mL), but unfortunately in my hand I couldn't see a strong and specific band corresponding to CHOP as I did with an antibody of a different trademark on the same blot.
Date published: 2023-12-22
Rated 5 out of 5 by from It's effective, it's specificIt's effective, it's specific, it's used for immunofluorescence
Date published: 2023-05-30
Rated 4 out of 5 by from GADD153 antibodyThe anti-GADD153 provided a clean, albeit weak signal, certainly due to poor expression in the tested cells.
Date published: 2023-04-19
Rated 4 out of 5 by from Good work!This antibody gave a strong and clean signal when we used it at a 1:200 diluition with 80μg of protein samples.
Date published: 2022-11-09
Rated 1 out of 5 by from not workingunfortunately, in our hands (or maybe in the cell line we have analyzed, that are endothelial cell lines) the antibody didn't worked.
Date published: 2021-10-26
Rated 5 out of 5 by from It worked greatIt works great without background. The dilution I used was 1:500.
Date published: 2021-10-25
Rated 5 out of 5 by from PerfectI tested this antibody in mouse liver tissues and hepatocytes. This worked perfectly.
Date published: 2021-05-25
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DDIT3/GADD153/CHOP 抗体 (B-3) is rated 4.1 out of 5 by 51.
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