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GABP-α Antibody (G-1) - Western Blotting - Image 355536 — GABP-α 抗体 (G-1): sc-28312. MCF7 (A), SW480 (B), Jurkat (C) 和 K-562 (D) 核提取物以及 SK-BR-3 (E) 和 3T3-L1 (F) 全细胞裂解液中 GABP-α 表达的 Western 印迹分析.

GABP-α 抗体 (G-1): sc-28312. MCF7 (A), SW480 (B), Jurkat (C) 和 K-562 (D) 核提取物以及 SK-BR-3 (E) 和 3T3-L1 (F) 全细胞裂解液中 GABP-α 表达的 Western 印迹分析.

GABP-α 抗体 (G-1): sc-28312

Name: GABP-&alpha;抗体 (G-1)
Brand: SCBT
SKU: sc-28312
Price: 316.0 USD
Availability: InStock

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说明书

GABP-alpha 抗体 G-1 是小鼠单克隆 IgG₁ κ，GABP-α抗体, 在19篇文献中引用，规格为200 µg/ml
免疫human物种的GABP-α的氨基酸1-180
GABP-alpha 抗体 (G-1) 推荐用于 WB, IP, IF 和 ELISA，检测mouse, rat 和human 来源的 GABP-α
抗GABP-alpha抗体（G-1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
还可偶联Alexa Fluor^® 488, Alexa Fluor^® 546, Alexa Fluor^® 594 和 Alexa Fluor^® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
还可偶联Alexa Fluor^® 680 和 Alexa Fluor^® 790，用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR^®/Odyssey^®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
可用于凝胶迁移和ChIP的TransCruz试剂(sc-28312 X, 200 µg/0.1 ml)
关于如何获取GABP-α (G-1): sc-28312的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
m-IgG Fc BP-HRP、 1 BP-HRP">m-IgG₁ BP-HRP和m-IgGκ BP-HRP是GABP-α Antibody (G-1) 适用于 WB 应用。的首选辅助检测试剂。这些试剂现与GABP-α Antibody (G-1) 打包提供（请参阅下面的订购信息）。

查看产品引用文献(19)

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GABP-α 抗体 (G-1) 参考文献:

hGABP 与活化转录因子/CAMP 反应元件结合蛋白家族的特定成员协同激活转录。 | Sawada, J., et al. 1999. J Biol Chem. 274: 35475-82. PMID: 10585419
GA 结合蛋白的 alpha 和 beta 亚基在溶液中形成稳定的异二聚体。修订的异源四聚体复合体组装模型。 | Chinenov, Y., et al. 2000. J Biol Chem. 275: 7749-56. PMID: 10713087
去极化刺激可上调神经元中的 GA 结合蛋白：一种参与细胞色素氧化酶亚基大体表达的转录因子。 | Zhang, C. and Wong-Riley, MT. 2000. Eur J Neurosci. 12: 1013-23. PMID: 10762332
GA 结合蛋白 alpha/beta 是 BRCA1 启动子的关键调节因子。 | Atlas, E., et al. 2000. Oncogene. 19: 1933-40. PMID: 10773883
ETS转录因子GABPalpha对早期胚胎发生至关重要。 | Ristevski, S., et al. 2004. Mol Cell Biol. 24: 5844-9. PMID: 15199140
鉴定小鼠 Gabp alpha/ATP 合酶偶联因子 6 双向启动子中的功能元件。 | Patton, J., et al. 2006. Gene. 369: 35-44. PMID: 16309857
GABPalpha 可调节小鼠胚胎干细胞中 Oct-3/4 的表达。 | Kinoshita, K., et al. 2007. Biochem Biophys Res Commun. 353: 686-91. PMID: 17194449
靶向 ETS 因子 GABPalpha 会破坏神经肌肉接头的突触功能。 | O'Leary, DA., et al. 2007. Mol Cell Biol. 27: 3470-80. PMID: 17325042
Neuregulin-1 在 GABPalpha 没有磷酸化的情况下诱导乙酰胆碱受体转录物。 | Herndon, CA. and Fromm, L. 2008. J Neurosci Res. 86: 982-91. PMID: 17975830
GABPalpha 是 T 细胞正常发育的关键条件。 | Yu, S., et al. 2010. J Biol Chem. 285: 10179-88. PMID: 20139079
转录因子人类 GA 结合蛋白亚基的功能相互作用。 | Suzuki, F., et al. 1998. J Biol Chem. 273: 29302-8. PMID: 9792629
通过将 NF-kappaB 增强子元件转化为 GABP 结合位点，实现人类免疫缺陷病毒 1 型长端重复启动子的进化。 | Verhoef, K., et al. 1999. J Virol. 73: 1331-40. PMID: 9882338

订购信息

产品名称	产品编号	规格	价格	数量	收藏夹
GABP-α 抗体 (G-1)	sc-28312	200 µg/ml	$316.00
GABP-α (G-1): m-IgG Fc BP-HRP 套装	sc-528417	200 µg Ab; 10 µg BP	$354.00
GABP-α (G-1): m-IgGκ BP-HRP 套装	sc-520791	200 µg Ab, 40 µg BP	$354.00
GABP-α (G-1): m-IgG₁ BP-HRP 套装	sc-542921	200 µg Ab; 20 µg BP	$354.00
GABP-α 抗体 (G-1) X	sc-28312 X	200 µg/0.1 ml	$316.00
GABP-α 抗体 (G-1) AC	sc-28312 AC	500 µg/ml, 25% agarose	$416.00
GABP-α 抗体 (G-1) HRP	sc-28312 HRP	200 µg/ml	$316.00
GABP-α 抗体 (G-1) FITC	sc-28312 FITC	200 µg/ml	$330.00
GABP-α 抗体 (G-1) PE	sc-28312 PE	200 µg/ml	$343.00
GABP-α 抗体 (G-1) Alexa Fluor^® 488	sc-28312 AF488	200 µg/ml	$357.00
GABP-α 抗体 (G-1) Alexa Fluor^® 546	sc-28312 AF546	200 µg/ml	$357.00
GABP-α 抗体 (G-1) Alexa Fluor^® 594	sc-28312 AF594	200 µg/ml	$357.00
GABP-α 抗体 (G-1) Alexa Fluor^® 647	sc-28312 AF647	200 µg/ml	$357.00
GABP-α 抗体 (G-1) Alexa Fluor^® 680	sc-28312 AF680	200 µg/ml	$357.00
GABP-α 抗体 (G-1) Alexa Fluor^® 790	sc-28312 AF790	200 µg/ml	$357.00

Can you provide a publication that used this antibody for IF staining? I can only find WB protocols and am having trouble getting the antibody to work for staining. Thank you!

Asked by: Anonymous

Thank you for contacting Santa Cruz Biotechnology. For assistance please contact technical service by emailing scbt@scbt.com. https://pubmed.ncbi.nlm.nih.gov/23197680/

Answered by: BlakeJ

Date published: 2025-02-21

Are there antibodies available for both the phosphorylated (Thr-280) and unphosphorylated forms of GABP alpha, and which does this antibody detect?

Asked by: CLangstaff

Thank you for your question. Anti-GABP-α Antibody (G-1): sc-28312 detects both phosphorylated and nonphosphorylated GABP-α. We currently do not offer any antibodies against this target that only recognize phosphorylated or only nonphosphorylated GABP-α.

Answered by: Tech Support Europe

Date published: 2022-09-05

Is there a blocking peptide for this antibody?

Asked by: AbPolly

Thank you for your question. Our GABP-a antibody sc-28312 is raised against a recombinant protein, not a short peptide sequence, so it is not possible to design a blocking peptide for it.

Answered by: Technical Support

Date published: 2017-01-30

Rated 5 out of 5 by Rani Vadlamudi from Great Supershift for EMSAI bought this antibody one month ago and observed great supershift band.

Date published: 2018-02-01

Rated 5 out of 5 by yunpengfei from good qualityWe compared the antibodies Abcam and Santa , and from the tests we found that no difference could be obtained, but the Santa's antibody was half the price of abcam. Under the same conditions, we are willing to prefer Santa antibodies. we suggest other users this antibody for 1:600 dilution.

Date published: 2017-07-22

Rated 5 out of 5 by Germaine from Highly recommended for WB.Great choice for detectng GABP-α of mouse, rat and human via Western Blotting

Date published: 2017-01-14

Rated 5 out of 5 by CJ from Used for EMSA with C2C12 cellsUsed for EMSA with C2C12 cells. -SCBT Publication Review

Date published: 2015-05-30

Rated 5 out of 5 by ET from Worked for ChIP analysis using K562 and JurkatWorked for ChIP analysis using K562 and Jurkat cells. -SCBT Publication Review

Date published: 2015-05-20

Rated 5 out of 5 by TO from Published ChIP data using HepG2 cellsPublished ChIP data using HepG2 cells. -SCBT Publication Review

Date published: 2015-03-24

Rated 5 out of 5 by SH from Published western blot data using rat PC12Published western blot data using rat PC12-ND6 cell lysates. -SCBT Publication Review

Date published: 2015-01-30

Rated 5 out of 5 by LM from Good antibody for western blotting; cleanGood antibody for western blotting; clean bands in HeLa, A-431, NIH/3T3 and 3611-RF nuclear extracts. -SCBT QC

Date published: 2013-07-24

GABP-α 抗体 (G-1) is rated 5.0 out of 5 by 8.

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种属	基因名称	基因ID	染色体位置	亚型（mRNA）登录号	蛋白质登录号	OMIM™ 号码
Human	GABPA	Entrez Gene ID: 2551	21q21.3	XM_005260938, XM_011529520, XM_011529521, XM_017028313, NM_001197297, NM_002040	UniProt ID: Q06546	600609
Mouse	Gabpa	Entrez Gene ID: 14390	16 C3.3	XM_006522905, XM_006522906, XM_006522907, XM_017316875, XM_017316876, NM_008065	UniProt ID: Q00422	N/A

GABP-α 抗体 (G-1): sc-28312

GABP-α 抗体 (G-1) 参考文献:

GABP-α 抗体 (G-1) 规格

替代产品名称: GABPA

产品克隆名称: G-1

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG₁ kappa light chain

氨基酸: 1-180

抗原表位靶标: GABP-α

抗原表位种属: human

产品反应性/检测: GABP-α

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 19篇各种科学出版物的引文

GABP-α 抗体 (G-1)

GABP-α (G-1): m-IgG Fc BP-HRP 套装

GABP-α (G-1): m-IgGκ BP-HRP 套装

GABP-α (G-1): m-IgG₁ BP-HRP 套装

GABP-α 抗体 (G-1) X

GABP-α 抗体 (G-1) AC

GABP-α 抗体 (G-1) HRP

GABP-α 抗体 (G-1) FITC

GABP-α 抗体 (G-1) PE

GABP-α 抗体 (G-1) Alexa Fluor^® 488

GABP-α 抗体 (G-1) Alexa Fluor^® 546

GABP-α 抗体 (G-1) Alexa Fluor^® 594

GABP-α 抗体 (G-1) Alexa Fluor^® 647

GABP-α 抗体 (G-1) Alexa Fluor^® 680

GABP-α 抗体 (G-1) Alexa Fluor^® 790

Can you provide a publication that used this antibody for IF staining? I can only find WB protocols and am having trouble getting the antibody to work for staining. Thank you!

Are there antibodies available for both the phosphorylated (Thr-280) and unphosphorylated forms of GABP alpha, and which does this antibody detect?

Is there a blocking peptide for this antibody?

GABP-α 抗体 (G-1): sc-28312

GABP-α 抗体 (G-1) 参考文献:

GABP-α 抗体 (G-1) 规格

替代产品名称: GABPA

产品克隆名称: G-1

分类: 一抗

Clonality: 单克隆抗体

抗体同型: IgG1 kappa light chain

氨基酸: 1-180

抗原表位靶标: GABP-α

抗原表位种属: human

产品反应性/检测: GABP-α

种属反应性/检测: mouse, rat, human

其它哺乳动物反应性: N/A

其它非哺乳动物反应性: N/A

应用: WB, IP, IF, ELISA

瓶的信息: Each vial contains 200 µg IgG1 kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

研究引文: 19篇各种科学出版物的引文

GABP-α 抗体 (G-1)

GABP-α (G-1): m-IgG Fc BP-HRP 套装

GABP-α (G-1): m-IgGκ BP-HRP 套装

GABP-α (G-1): m-IgG1 BP-HRP 套装

GABP-α 抗体 (G-1) X

GABP-α 抗体 (G-1) AC

GABP-α 抗体 (G-1) HRP

GABP-α 抗体 (G-1) FITC

GABP-α 抗体 (G-1) PE

GABP-α 抗体 (G-1) Alexa Fluor® 488

GABP-α 抗体 (G-1) Alexa Fluor® 546

GABP-α 抗体 (G-1) Alexa Fluor® 594

GABP-α 抗体 (G-1) Alexa Fluor® 647

GABP-α 抗体 (G-1) Alexa Fluor® 680

GABP-α 抗体 (G-1) Alexa Fluor® 790

Can you provide a publication that used this antibody for IF staining? I can only find WB protocols and am having trouble getting the antibody to work for staining. Thank you!

Are there antibodies available for both the phosphorylated (Thr-280) and unphosphorylated forms of GABP alpha, and which does this antibody detect?

Is there a blocking peptide for this antibody?

抗体同型: IgG₁ kappa light chain

瓶的信息: Each vial contains 200 µg IgG₁ kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

GABP-α (G-1): m-IgG₁ BP-HRP 套装

GABP-α 抗体 (G-1) Alexa Fluor^® 488

GABP-α 抗体 (G-1) Alexa Fluor^® 546

GABP-α 抗体 (G-1) Alexa Fluor^® 594

GABP-α 抗体 (G-1) Alexa Fluor^® 647

GABP-α 抗体 (G-1) Alexa Fluor^® 680

GABP-α 抗体 (G-1) Alexa Fluor^® 790