FXR/NR1H4 Antibody (D-3): sc-25309

FXR Antibody (D-3) is a mouse monoclonal IgG2a kappa light chain antibody that detects FXR protein of mouse, rat, and human origin by western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), and enzyme-linked immunosorbent assay (ELISA). Anti-FXR antibody (D-3) is available in both non-conjugated and various conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and multiple Alexa Fluor® conjugates. FXR, also known as NR1H4, plays a crucial role in regulating bile acid homeostasis and lipid metabolism, making FXR essential for maintaining metabolic health. Located primarily in the liver and intestine, FXR functions as a nuclear receptor that, upon binding to ligands, regulates the expression of genes involved in bile acid synthesis and transport. This regulation is vital because dysregulation of bile acids can lead to metabolic disorders, liver diseases, and other health issues. FXR forms heterodimers with retinoid X receptor (RXR) and binds to specific DNA sequences known as response elements, thereby influencing gene transcription. FXR′s ability to interact with various lipid molecules, including farnesoids, further underscores FXR′s importance in metabolic signaling pathways. With diverse applications and critical role in metabolic regulation, anti-FXR antibody (D-3) serves as an invaluable tool for researchers studying the complexities of lipid metabolism and related diseases.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

FXR/NR1H4 Antibody (D-3) References:

1. Bile acid nuclear receptor FXR and digestive system diseases.  |  Ding, L., et al. 2015. Acta Pharm Sin B. 5: 135-44. PMID: 26579439
2. FXR Regulates Intestinal Cancer Stem Cell Proliferation.  |  Fu, T., et al. 2019. Cell. 176: 1098-1112.e18. PMID: 30794774
3. FXR activation protects against NAFLD via bile-acid-dependent reductions in lipid absorption.  |  Clifford, BL., et al. 2021. Cell Metab. 33: 1671-1684.e4. PMID: 34270928
4. FXR: structures, biology, and drug development for NASH and fibrosis diseases.  |  Tian, SY., et al. 2022. Acta Pharmacol Sin. 43: 1120-1132. PMID: 35217809
5. FXR mediates ILC-intrinsic responses to intestinal inflammation.  |  Fu, T., et al. 2022. Proc Natl Acad Sci U S A. 119: e2213041119. PMID: 36508655
6. FXR agonists for MASH therapy: Lessons and perspectives from obeticholic acid.  |  Wang, K., et al. 2024. Med Res Rev. 44: 568-586. PMID: 37899676
7. 9-cis retinoic acid signaling: changing partners causes some excitement.  |  Leblanc, BP. and Stunnenberg, HG. 1995. Genes Dev. 9: 1811-6. PMID: 7649469
8. LXR, a nuclear receptor that defines a distinct retinoid response pathway.  |  Willy, PJ., et al. 1995. Genes Dev. 9: 1033-45. PMID: 7744246
9. Identification of a nuclear receptor that is activated by farnesol metabolites.  |  Forman, BM., et al. 1995. Cell. 81: 687-93. PMID: 7774010
10. The dimerization interfaces formed between the DNA binding domains of RXR, RAR and TR determine the binding specificity and polarity of the full-length receptors to direct repeats.  |  Zechel, C., et al. 1994. EMBO J. 13: 1425-33. PMID: 8137826
11. The nuclear receptor superfamily: the second decade.  |  Mangelsdorf, DJ., et al. 1995. Cell. 83: 835-9. PMID: 8521507
12. The RXR heterodimers and orphan receptors.  |  Mangelsdorf, DJ. and Evans, RM. 1995. Cell. 83: 841-50. PMID: 8521508

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-FXR/NR1H4 Antibody (D-3) has 74 citations in a variety of scientific publications.

Primary antibodies like Anti-FXR/NR1H4 Antibody (D-3) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

FXR/NR1H4 Antibody (D-3) Specifications

Alternate Product Name: FXR

Product Clone Name: D-3

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-130

Epitope Target: FXR

Epitope Species: human

Product Reactivity/Detection: FXR

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 74 citations in various scientific publications

Available Conjugates/Forms for FXR/NR1H4 Antibody (D-3): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin, X: TransCruz Reagent