Date published: 2025-9-8

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Flotillin-1 抗体 (C-7): sc-133153

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说明书
  • Flotillin-1抗体(C-7)是小鼠单克隆IgG1 κ, 在22篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Flotillin-1的氨基酸324-427
  • 抗-Flotillin-1 抗体 (C-7) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Flotillin-1
  • 抗Flotillin-1抗体(C-7)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Flotillin-1 (C-7): sc-133153免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP1 BP-HRP">m-IgG1 BP-HRP是Flotillin-1 Antibody (C-7) for WB applications. 的首选辅助检测试剂,这些试剂现与Flotillin-1 Antibody (C-7) 打包提供(请参阅下面的订购信息)。

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関連項目

Flotillin-1抗体(C-7)是一种IgG1 κ小鼠单克隆Flotillin-1抗体(也称为Flotillin-1抗体或FLOT1抗体),可通过WB(蛋白质印迹)、IP(免疫沉淀)、IF(免疫荧光)和ELISA(酶联免疫吸附测定)检测来自小鼠、大鼠和人的Flotillin-1。Flotillin-1抗体(C-7)以非偶联抗Flotillin-1抗体形式以及多种偶联形式的抗Flotillin-1抗体提供,包括琼脂糖、HRP(辣根过氧化物酶)、PE(藻红蛋白)、FITC(异硫氰酸荧光素)和多种Alexa Fluor®偶联物。脂质筏是富含鞘脂和胆固醇的膜微区,不溶于非离子洗涤剂。脂质筏对许多细胞过程都很重要,包括信号转导、膜转运和分子分选。Flotillins是神经元中的脂质筏成分,也是A498肾细胞中的小窝相关蛋白。Flotillin-1属于band 7.2/stomatin蛋白家族,其成员的特征是存在疏水性N端区域,该区域预计会形成单个从外到内的跨膜结构域。Flotillin-1和-2具有互补的组织分布,并且它们的表达水平是独立调节的。在细胞水平上,Flotillin-2普遍表达,而Flotillin-1则在A498肾细胞、肌肉细胞系和成纤维细胞中表达。Flotillins与CAP和Cbl形成三元复合物,将CAP-Cbl复合物的定位导向质膜脂质筏子域。ER-X与Flotillin的结合将ER-X定位在质膜小窝内,并介导雌激素对MAP激酶级联的快速激活。Flotillins的表达也与阿尔茨海默氏症病理的进展相关。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Flotillin-1 抗体 (C-7)

sc-133153
200 µg/ml
$316.00

Flotillin-1 (C-7): m-IgG Fc BP-HRP 套装

sc-527096
200 µg Ab; 10 µg BP
$354.00

Flotillin-1 (C-7): m-IgG1 BP-HRP 套装

sc-532469
200 µg Ab; 20 µg BP
$354.00

Flotillin-1 抗体 (C-7) AC

sc-133153 AC
500 µg/ml, 25% agarose
$416.00

Flotillin-1 抗体 (C-7) HRP

sc-133153 HRP
200 µg/ml
$316.00

Flotillin-1 抗体 (C-7) FITC

sc-133153 FITC
200 µg/ml
$330.00

Flotillin-1 抗体 (C-7) PE

sc-133153 PE
200 µg/ml
$343.00

Flotillin-1 抗体 (C-7) Alexa Fluor® 488

sc-133153 AF488
200 µg/ml
$357.00

Flotillin-1 抗体 (C-7) Alexa Fluor® 546

sc-133153 AF546
200 µg/ml
$357.00

Flotillin-1 抗体 (C-7) Alexa Fluor® 594

sc-133153 AF594
200 µg/ml
$357.00

Flotillin-1 抗体 (C-7) Alexa Fluor® 647

sc-133153 AF647
200 µg/ml
$357.00

Flotillin-1 抗体 (C-7) Alexa Fluor® 680

sc-133153 AF680
200 µg/ml
$357.00

Flotillin-1 抗体 (C-7) Alexa Fluor® 790

sc-133153 AF790
200 µg/ml
$357.00

What is the recommended fixation method for immunofluorescence staining with Flotillin-1 (C-7): sc-133153 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
  • y_2025, m_9, d_4, h_6CST
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Rated 5 out of 5 by from Good antibodyI bought this antibody one month ago. This antibody worked very well with a clean, single band on the western blot Expr. Love this antibody
Date published: 2021-01-12
Rated 5 out of 5 by from Good for western blotThe antibody was tested by western blot in lipid rafts fractions from endothelial cells. It works well.
Date published: 2018-01-31
Rated 5 out of 5 by from Highly recommended for Flotillin-1!This is an excellent antibody for Western blot: nice specific bands every time. Great choice!
Date published: 2017-01-23
Rated 4 out of 5 by from Good specificity in WB in KGood specificity in WB in K-562, A-431 and Jurkat whole cell lysates. -SCBT QC
Date published: 2015-06-28
Rated 4 out of 5 by from Robust signal in WB in human transfectedRobust signal in WB in human transfected 293T whole cell lysates. -SCBT QC
Date published: 2015-04-07
Rated 3 out of 5 by from Not one of the better antibodies I've usedNot one of the better antibodies I've used but I obtained the results I needed.
Date published: 2015-03-25
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Flotillin-1抗体 (C-7) is rated 4.3 out of 5 by 6.
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