Date published: 2025-9-6
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Estrogen Receptor alpha Antibody (D-12) - Western Blotting - Image 376606
雌激素受体 α (D-12): sc-8005. T-47D (A) 和 c4 (B) 全细胞裂解液中雌激素受体 α 表达的 Western 印迹分析.
雌激素受体 α (D-12): sc-8005. T-47D (A) 和 c4 (B) 全细胞裂解液中雌激素受体 α 表达的 Western 印迹分析.

Estrogen Receptor alpha 抗体 (D-12): sc-8005

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说明书
  • Estrogen Receptor alpha抗体(D-12)是小鼠单克隆IgG2a κ, 在290篇文献中引用，规格为200 µg/ml
  • 免疫human来源Estrogen Receptor alpha位于N-terminus 的2-185氨基酸
  • 抗-Estrogen Receptor alpha 抗体 (D-12) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat 和human 来源的 Estrogen Receptor alpha
  • 抗Estrogen Receptor alpha抗体（D-12）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联TRITC 用于IF, IHC(P) 和FCM
  • 可用于凝胶迁移和ChIP的TransCruz试剂(sc-8005 X, 200 µg/0.1 ml)
  • 关于如何获取Estrogen Receptor alpha (D-12): sc-8005的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP 和 m-IgG2a BP-HRP 是 Estrogen Receptor alpha 抗体 (D-12) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Estrogen Receptor alpha 抗体 (D-12) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Estrogen Receptor 抗体 包括 Estrogen Receptor alpha, ER β, ERR α, ERR β和ERR γ

雌激素受体α抗体（D-12）是一种小鼠单克隆IgG2a kappa轻链抗体，针对人类雌激素受体α（ERα）N端2-185氨基酸。ERα单克隆抗体（D-12）可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、石蜡包埋切片免疫组织化学（IHCP）和酶联免疫吸附测定（ELISA）检测小鼠、大鼠和人类来源的ERα。ERα蛋白主要位于雌激素反应细胞核中，作为类固醇/甲状腺激素受体超家族中的配体激活转录因子发挥作用。ERα的核定位至关重要，因为ERα允许在雌激素反应元件（EREs）处与DNA直接相互作用，从而调节细胞增殖、分化以及生殖功能相关基因的转录。这种定位对于调节雌激素的生理效应（包括正常的性发育和生殖健康）至关重要。ERα的定位错误或失调会导致基因表达改变，并与多种疾病有关，例如乳腺癌等激素依赖性癌症。ERα单克隆抗体（D-12）有非结合型和多种结合型，包括HRP、FITC、琼脂糖、PE和各种Alexa Fluor®结合物，为研究ERα的功能、定位和在疾病中的作用提供了广泛的研究应用。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

订购信息

产品名称产品编号规格价格数量收藏夹

Estrogen Receptor alpha 抗体 (D-12)

sc-8005
200 µg/ml
$316.00

Estrogen Receptor alpha (D-12): m-IgG Fc BP-HRP 套装

sc-525445
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha (D-12): m-IgG2a BP-HRP 套装

sc-546259
200 µg Ab; 10 µg BP
$354.00

Estrogen Receptor alpha 抗体 (D-12) X

sc-8005 X
200 µg/0.1 ml
$316.00

Estrogen Receptor alpha 抗体 (D-12) AC

sc-8005 AC
500µg/1ml, 25% agarose
$416.00

Estrogen Receptor alpha 抗体 (D-12) HRP

sc-8005 HRP
200 µg/ml
$316.00

Estrogen Receptor alpha 抗体 (D-12) FITC

sc-8005 FITC
200 µg/ml
$330.00

Estrogen Receptor alpha 抗体 (D-12) PE

sc-8005 PE
200 µg/ml
$343.00

Estrogen Receptor alpha 抗体 (D-12) Alexa Fluor® 488

sc-8005 AF488
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (D-12) Alexa Fluor® 546

sc-8005 AF546
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (D-12) Alexa Fluor® 594

sc-8005 AF594
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (D-12) Alexa Fluor® 647

sc-8005 AF647
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (D-12) Alexa Fluor® 680

sc-8005 AF680
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (D-12) Alexa Fluor® 790

sc-8005 AF790
200 µg/ml
$357.00

Estrogen Receptor alpha 抗体 (D-12) TRITC

sc-8005 TRITC
200 µg/ml
$320.00

I would like to know if this antibody recognize the whole sequence from 2- 185, or just part of the sequence within?

Asked by: Anonymous
Thank you for your question. Estrogen Receptor alpha Antibody (D-12): sc-8005 was raised against amino acids 2-185 mapping at the N-terminus of Estrogen Receptor alpha of human origin. Its binding site is one specific point within this epitope region.
Answered by: Tech Support Europe
Date published: 2020-07-02

Hi, I want to do IHC for formalin fixed rat mammary gland, does this antibody work for rat tissues by IHC?

Asked by: Yanrong
Thank you for your question. Yes, this antibody is recommended for IHC(P) in mouse, rat and human tissues.
Answered by: Technical Support
Date published: 2019-08-17

Can we use this antibody for IF and IHC staining in paraffin-embedded mouse tissues?

Asked by: YuHsin
Thank you for your question. ERα Antibody (D-12): sc-8005 is recommended for detection of ERα of mouse, rat and human origin by WB, IP, IF, IHC(P) and ELISA, so using it for IF or IHC(P) staining should work well. For published data with this antibody in murine tissues and IHC(P) please see PMID 19332863 or PMID 22992387.
Answered by: Tech Support Europe
Date published: 2019-07-03

Can we use this antibody in rat brain samples for W.B. Immunoflourescence in frozen sections?

Asked by: Rukmani p
Thank you for your question. This mouse monoclonal antibody, ERα (D-12): sc-8005, is suitable for Western blotting (WB) and immunoflourescence (IF) in frozen tissues of rat origin. We have not tested this antibody specifically in rat brain tissue samples. Please refer to the data on our website for our recommended positive controls to include when testing this antibody in your samples.
Answered by: Technical Support
Date published: 2017-08-03

Can I use this antibody for immunoprecipitation of ER alpha from mouse brain?

Asked by: Victor86
Thank you for your question. This mouse monoclonal antibody, ERα (D-12): sc-8005, can be used for immunoprecipitation (IP) in mouse samples. We are not aware of any specific publications citing use of the antibody to IP ER alpha from brain tissue samples, but it has been successfully used for IP in both human and mouse cell lines.
Answered by: Technical Support
Date published: 2017-05-18

I need to use an antibody to detect the protein in samples of canineorigin. Do you know if this mouse monoclonal antibody will work?

Asked by: jerojero
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Tech Service 3
Date published: 2016-12-21

Can I use this mouse monoclonal antibody for immunohistochemical staining of mouse tissue?

Asked by: DefinitelyNotMatt
Thank you for your question. Yes. We recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is currently available conjugated to Fluorescein (sc-8005 FITC) and TRITC (sc-8005 TRITC). Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15

What is the recommended secondary reagent to be used with this mouse monoclonal primary?

Asked by: DefinitelyNotMatt
Thank you for your question. We recommend using one our exclusive Mouse IgG Binding Proteins as a secondary detection reagent. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety Please contact Technical Service if you have further questions concerning this product.
Answered by: Tech Service 9
Date published: 2016-12-15
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Rated 5 out of 5 by from Great IHC results on mouse brain tiisueAntigen retrieval with TE buffer works really great!
Date published: 2022-07-31
Rated 5 out of 5 by from Great ProductAn excellent product for the lab's research. Highly recommended for future uses.
Date published: 2022-07-13
Rated 3 out of 5 by from Ok, it workedWe tried the free sample on wester blot at the suggested dilution 1:200 on mouse samples. It worked, but compared to the old policlonal MC-20the result isn't good as before.
Date published: 2021-12-21
Rated 4 out of 5 by from ChIPWorking well so far for my current ChIP experiments.
Date published: 2020-12-28
Rated 3 out of 5 by from good antibodygood antibody for western blotting and also immunofluorescence
Date published: 2019-01-21
Rated 2 out of 5 by from Unsure of molecular weight in Western blotA very faint band showed up at the correct molecular weight. A brighter band showed up at the incorrect molecular weight. Western Blot (1:100) in rat white adipose tissue.
Date published: 2018-09-12
Rated 4 out of 5 by from Good antibodyI tried it on cultured human cells on IF (1:100, 1:200), both concentration worked.
Date published: 2018-04-07
Rated 5 out of 5 by from GreatI had great signal for ICC on Human breast cancer cell lines.
Date published: 2018-02-27
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Estrogen Receptor alpha 抗体 (D-12) is rated 3.9 out of 5 by 24.
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