Date published: 2025-9-6
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Epac Antibody (A-5) - Western Blotting - Image 358554
Epac 抗体 (A-5): sc-28366. 在 SK-N-MC (A), IMR-32 (B), BC3H1 (C), EOC 20 (D), PC-12 (E) 和 C6 (F) 全细胞裂解液中 Epac 表达的 Western 印迹分析.
Epac 抗体 (A-5): sc-28366. 在 SK-N-MC (A), IMR-32 (B), BC3H1 (C), EOC 20 (D), PC-12 (E) 和 C6 (F) 全细胞裂解液中 Epac 表达的 Western 印迹分析.

Epac 抗体 (A-5): sc-28366

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寫評論提問

说明书
  • Epac 抗体 A-5 是小鼠单克隆 IgG2a κ，Epac抗体, 在17篇文献中引用，规格为200 µg/ml
  • 免疫human物种的Epac的氨基酸1-70
  • Epac 抗体 (A-5) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA，检测mouse, rat, human 和hamster 来源的 Epac
  • 抗Epac抗体（A-5）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Epac (A-5): sc-28366的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRP 和 m-IgGκ BP-HRP 是 Epac 抗体 (A-5) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Epac 抗体 (A-5) 组合成套装提供。 (请参阅下面的订购信息)。

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相关产品
描述
基因信息
蛋白序列
说明书与实验方案
研究信息

関連項目

此外 Epac 抗体包括 Epac2

Epac抗体（A-5）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和酶联免疫吸附测定（ELISA）检测小鼠、大鼠、人类和仓鼠的Epac蛋白。 酶联免疫吸附试验（ELISA）。抗Epac抗体（A-5）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。Epac蛋白（又称cAMP直接激活的交换蛋白1）在细胞信号传导中起着至关重要的作用，它是一种鸟嘌呤核苷酸交换因子，能够以cAMP依赖的方式激活Ras家族GTP酶Rap 1和Rap 2。这种激活对于调节各种生理反应至关重要，包括基因表达、细胞生长、分化、分泌和神经传递，这些对于维持正常的细胞功能和对外部刺激的响应至关重要。人类Epac基因位于12q13.11号染色体上，其转录产物在肾脏和心脏中高度表达，原位杂交研究显示，在成年大鼠脑中，尤其是在新生儿发育期间的隔膜和丘脑等区域，也有表达。这些发现强调了Epac在发育和成人生理过程中的重要性，使Epac（A-5）抗体成为研究人员研究cAMP信号通路及其对健康和疾病的影响的有价值的工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Epac 抗体 (A-5) 参考文献:

  1. cAMP 依赖性 RapGEFs 的 Epac 家族的调控机制。  |  de Rooij, J., et al. 2000. J Biol Chem. 275: 20829-36. PMID: 10777494
  2. 基因 EPAC2 的特征：结构, 染色体定域, 组织表达和肝脏特异性同工酶的鉴定。  |  Ueno, H., et al. 2001. Genomics. 78: 91-8. PMID: 11707077
  3. 胰腺β细胞中的 Ca2+ 传感器 Piccolo。cAMP-GEFII.Rim2.Piccolo 复合物在 cAMP 依赖性外吞过程中的参与。  |  Fujimoto, K., et al. 2002. J Biol Chem. 277: 50497-502. PMID: 12401793
  4. Epac在心脏中的作用。  |  Fujita, T., et al. 2017. Cell Mol Life Sci. 74: 591-606. PMID: 27549789
  5. Epac和痛觉感受器敏化。  |  Huang, LY. and Gu, Y. 2017. Mol Pain. 13: 1744806917716234. PMID: 28580839
  6. 环磷酸腺苷依赖性蛋白激酶A和EPAC介导VIP和胰泌素对PAK4的刺激，并激活胰腺小管细胞中的Na+，K+-ATP酶。  |  Ramos-Alvarez, I., et al. 2019. Am J Physiol Gastrointest Liver Physiol. 316: G263-G277. PMID: 30520694
  7. 选择性小分子EPAC激活剂。  |  Luchowska-Stańska, U., et al. 2019. Biochem Soc Trans. 47: 1415-1427. PMID: 31671184
  8. 血管平滑肌细胞中的EPAC。  |  Wehbe, N., et al. 2020. Int J Mol Sci. 21: PMID: 32708284
  9. Epac在癌症进展中的作用。  |  Wehbe, N., et al. 2020. Int J Mol Sci. 21: PMID: 32899451
  10. Epac作为可治疗靶点。  |  Slika, H., et al. 2023. Eur J Pharmacol. 945: 175645. PMID: 36894048
  11. cAMP-PKA/EPAC信号传导与癌症：肿瘤微环境中的相互作用。  |  Zhang, H., et al. 2024. J Hematol Oncol. 17: 5. PMID: 38233872
  12. 直接激活 Rap1 的 cAMP 结合蛋白家族。  |  Kawasaki, H., et al. 1998. Science. 282: 2275-9. PMID: 9856955

订购信息

产品名称产品编号规格价格数量收藏夹

Epac 抗体 (A-5)

sc-28366
200 µg/ml
$316.00

Epac (A-5): m-IgG Fc BP-HRP 套装

sc-528436
200 µg Ab; 10 µg BP
$354.00

Epac (A-5): m-IgGκ BP-HRP 套装

sc-520811
200 µg Ab, 40 µg BP
$354.00

Epac (A-5): m-IgG2a BP-HRP 套装

sc-547096
200 µg Ab; 10 µg BP
$354.00

Epac 抗体 (A-5) AC

sc-28366 AC
500 µg/ml, 25% agarose
$416.00

Epac 抗体 (A-5) HRP

sc-28366 HRP
200 µg/ml
$316.00

Epac 抗体 (A-5) FITC

sc-28366 FITC
200 µg/ml
$330.00

Epac 抗体 (A-5) PE

sc-28366 PE
200 µg/ml
$343.00

Epac 抗体 (A-5) Alexa Fluor® 488

sc-28366 AF488
200 µg/ml
$357.00

Epac 抗体 (A-5) Alexa Fluor® 546

sc-28366 AF546
200 µg/ml
$357.00

Epac 抗体 (A-5) Alexa Fluor® 594

sc-28366 AF594
200 µg/ml
$357.00

Epac 抗体 (A-5) Alexa Fluor® 647

sc-28366 AF647
200 µg/ml
$357.00

Epac 抗体 (A-5) Alexa Fluor® 680

sc-28366 AF680
200 µg/ml
$357.00

Epac 抗体 (A-5) Alexa Fluor® 790

sc-28366 AF790
200 µg/ml
$357.00

Can Epac (A-5): sc-28366 monoclonal antibody be used in IF or IHC with mouse tissue or cells? Will there be non-specific staining?

Asked by: Professor Griffin
Thank you for your inquiry. The use of mouse monoclonal antibodies with mouse samples is very common and typically poses no problem. To eliminate any potential cross-reactivity of an anti-mouse secondary detection reagent, Epac (A-5): sc-28366 is available in a variety of direct conjugations, such as HRP, PE, FITC and AlexaFluors.
Answered by: Technical Support
Date published: 2017-02-24

Does this recognize epac1 or epac2 or both?

Asked by: Marina1
Thank you for your question. This mouse monoclonal antibody, Epac (A-5): sc-28366, is raised against the gene product of RAPGEF3, also designated Epac or Epac1. The Uniprot accession number for the full length protein is O95398. You can find a description with links to NCBI for the gene and protein information on our product page, under the tab "Gene Info" next to "Description" about half way down the page (below the ordering information). https://www.scbt.com/scbt/product/epac-antibody-a-5
Answered by: Technical Support
Date published: 2016-11-21
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Rated 4 out of 5 by from Works well in bovine cell lysatesI tested this antibody on bovine cell lysates and it works well. There are some smaller bands that show up maybe do to protein degradation.
Date published: 2017-10-04
Rated 5 out of 5 by from Great antibodyIt really worked nicely, shown nice bands at 100 kDa at 1:500 dilution.
Date published: 2017-04-09
Rated 5 out of 5 by from Beautiful resultsThis antibody works well in standard lab applications and will provide great results in samples from several species. It has been published with several times.
Date published: 2017-01-27
Rated 5 out of 5 by from Worked better than scWorked better than sc-25632 for WB using 293 cells that over-express protein.
Date published: 2015-09-24
Rated 4 out of 5 by from This monoclonal was betterThis monoclonal was better, detecting Epac, although its strongest signal is not Epac.
Date published: 2015-07-13
Rated 4 out of 5 by from Strong band in WB in Caki and CHO whole cellStrong band in WB in Caki and CHO whole cell lysates. -SCBT QC -SCBT QC
Date published: 2015-06-30
Rated 4 out of 5 by from Good publishable data in IHC and WB in humanGood publishable data in IHC and WB in human TR4 cells. -SCBT Publication Review
Date published: 2015-05-30
Rated 5 out of 5 by from Produced beautiful immunoperoxidase cytoplasmicProduced beautiful immunoperoxidase cytoplasmic staining in formalin fixed, paraffin-embedded human salivary gland tissue. -SCBT QC
Date published: 2015-05-11
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Epac 抗体 (A-5) is rated 4.4 out of 5 by 10.
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