Date published: 2025-9-6

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Dynamin 抗体 (E-11): sc-17807

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说明书
  • Dynamin 抗体 E-11 是小鼠单克隆 IgG2a κ,Dynamin抗体, 在4篇文献中引用,规格为200 µg/ml
  • 免疫human来源Dynamin位于C-terminus 的567-866氨基酸
  • Dynamin 抗体 (E-11) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 Dynamin
  • 抗Dynamin抗体(E-11)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Dynamin (E-11): sc-17807免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是Dynamin Antibody (E-11) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与Dynamin Antibody (E-11) 打包提供(请参阅下面的订购信息)。

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関連項目

Dynamin抗体(E-11)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA)检测小鼠、大鼠和人类来源的Dynamin蛋白。抗 -Dynamin 抗体(E-11)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物。Dynamin蛋白在胞吞作用、突触传递和神经发生中起着至关重要的作用,因此Dynamin对于细胞通讯和营养吸收至关重要。具体来说,Dynamin I主要定位于中枢神经系统,而Dynamin II广泛分布于全身,在睾丸中的表达量最高。这两种同源物都含有SH3和富含脯氨酸的域,可促进与各种效应子的相互作用,包括微管和含有SH3域的蛋白,这些蛋白对于快速内吞作用至关重要。值得注意的是,Dynamin I通过与amphiphysin相互作用而被招募到包被有clathrin的凹坑中,amphiphysin是一种在大脑中高度表达的蛋白,这强调了这些相互作用在神经元功能和信号传导中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Dynamin 抗体 (E-11) 参考文献:

  1. 调节内吞过程中的动态动态平衡  |  Sundborger, AC. and Hinshaw, JE. 2014. F1000Prime Rep. 6: 85. PMID: 25374663
  2. Dynamin 功能和配体:经典机制的背后。  |  Singh, M., et al. 2017. Mol Pharmacol. 91: 123-134. PMID: 27879341
  3. Dynamin 在内吞点为超快速内吞作用提供了条件。  |  Imoto, Y., et al. 2022. Neuron. 110: 2815-2835.e13. PMID: 35809574
  4. 依赖 Dynamin 的内吞和受体贩运机制  |  Gundu, C., et al. 2022. Cells. 11: PMID: 36010634
  5. 生长因子诱导的达纳明与信号转导蛋白的结合涉及到对不同的富含脯氨酸的达纳明序列的分选。  |  Scaife, R., et al. 1994. EMBO J. 13: 2574-82. PMID: 8013457
  6. 多种动态蛋白的差异表达和调控。  |  Sontag, JM., et al. 1994. J Biol Chem. 269: 4547-54. PMID: 8308025
  7. SH3结构域的结合位点可将动态蛋白靶向涂布坑。  |  Shpetner, HS., et al. 1996. J Biol Chem. 271: 13-6. PMID: 8550547
  8. 达纳明富含脯氨酸的基本区域在 Src 同源 3 结构域结合和内吞过程中的作用  |  Okamoto, PM., et al. 1997. J Biol Chem. 272: 11629-35. PMID: 9111080
  9. 虹彩蛋白的 SH3 结构域与达能蛋白的富脯氨酸结构域结合在一个单一位点上,该位点定义了一个新的 SH3 结合共识序列。  |  Grabs, D., et al. 1997. J Biol Chem. 272: 13419-25. PMID: 9148966
  10. 虹彩蛋白 SH3 结构域对受体介导的内吞作用的抑制。  |  Wigge, P., et al. 1997. Curr Biol. 7: 554-60. PMID: 9259551
  11. PH 结构域和 SH3 结合结构域在达能素功能中的作用  |  Scaife, RM. and Margolis, RL. 1997. Cell Signal. 9: 395-401. PMID: 9376220
  12. 鉴定大鼠组织中普遍表达的同工酶达纳明 2。  |  Cook, TA., et al. 1994. Proc Natl Acad Sci U S A. 91: 644-8. PMID: 8290576

订购信息

产品名称产品编号规格价格数量收藏夹

Dynamin 抗体 (E-11)

sc-17807
200 µg/ml
$316.00

Dynamin (E-11): m-IgG Fc BP-HRP 套装

sc-528320
200 µg Ab; 10 µg BP
$354.00

Dynamin (E-11): m-IgGκ BP-HRP 套装

sc-520665
200 µg Ab, 40 µg BP
$354.00

Dynamin (E-11): m-IgG2a BP-HRP 套装

sc-547071
200 µg Ab; 10 µg BP
$354.00

Dynamin 抗体 (E-11) AC

sc-17807 AC
500 µg/ml, 25% agarose
$416.00

Dynamin 抗体 (E-11) HRP

sc-17807 HRP
200 µg/ml
$316.00

Dynamin 抗体 (E-11) FITC

sc-17807 FITC
200 µg/ml
$330.00

Dynamin 抗体 (E-11) PE

sc-17807 PE
200 µg/ml
$343.00

Dynamin 抗体 (E-11) Alexa Fluor® 488

sc-17807 AF488
200 µg/ml
$357.00

Dynamin 抗体 (E-11) Alexa Fluor® 546

sc-17807 AF546
200 µg/ml
$357.00

Dynamin 抗体 (E-11) Alexa Fluor® 594

sc-17807 AF594
200 µg/ml
$357.00

Dynamin 抗体 (E-11) Alexa Fluor® 647

sc-17807 AF647
200 µg/ml
$357.00

Dynamin 抗体 (E-11) Alexa Fluor® 680

sc-17807 AF680
200 µg/ml
$357.00

Dynamin 抗体 (E-11) Alexa Fluor® 790

sc-17807 AF790
200 µg/ml
$357.00

Hii. just wanted to let you know, if i can get free sample of Dynamin antibody. Thanks

Asked by: mumtaz
For a limited time, we are offering a free 10 µg sample of this antibody. Please contact our Technical Service Department if you would like to test the antibody before placing your order. Please call us toll-free at (800) 457-3801 option 2, e-mail <scbt@scbt.com>, or use the live chat on our website.
Answered by: Technical Support
Date published: 2017-10-31

What is the recommended fixation method for immunofluorescence staining with Dynamin (E-11): sc-17807 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from ENDOGENOUS DYNAMIN DETECTION (HEK-293T cells)sc-17807 specifically recognizes endogenous dynamin in HEK-293T cells. Detection was made using 1:500 antibody dilution for 16 hours at 4C to probe samples originating from HEK-293T whole cell lysates. Samples were analyzed on 12% SDS-PAGE. IRDye 800WC was used as a secondary fluorescent antibody captured on LiCOR Odyssey XL.
Date published: 2022-09-07
Rated 4 out of 5 by from clear bands in WBAntibody sample has been used for WB of HeLa cell lysate. Incubation O/N at 4°C, dilution 1/200. Worked very nice, had a clear band at 100 kDa, no unspecific bands.
Date published: 2018-05-11
Rated 5 out of 5 by from Works as expected in WBgood, pretty clean bands for WB in human brain and rat hippocampus tissue extracts at a 1:200 dilution
Date published: 2017-02-08
Rated 5 out of 5 by from Strong band of interest detected in WB in SKStrong band of interest detected in WB in SK-N-SH, IMR-32 and U-87 MG whole cell lysates. -SCBT QC
Date published: 2015-05-24
Rated 5 out of 5 by from Positive result in WB in mouse brain tissuePositive result in WB in mouse brain tissue extract. -SCBT QC
Date published: 2015-02-26
Rated 5 out of 5 by from Published data for WB in monkey COS7 cellsPublished data for WB in monkey COS7 cells. -SCBT Publication Review
Date published: 2015-02-22
Rated 5 out of 5 by from Produced excellent immunofluorescence cytoplasmicProduced excellent immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2013-05-04
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Dynamin 抗体 (E-11) is rated 4.9 out of 5 by 7.
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