CREM Antibody (C-2): sc-390426

CREM Antibody (C-2) is a mouse monoclonal IgG2a kappa light chain antibody specifically developed to recognize the cAMP response element modulator (CREM). Anti-CREM antibody (C-2) targets the N-terminal region of CREM, encompassing amino acids 1-153, and is validated for use in western blotting (WB), immunoprecipitation (IP), immunofluorescence (IF), immunohistochemistry with paraffin-embedded sections (IHCP), and enzyme-linked immunosorbent assay (ELISA) across mouse, rat, and human species. CREM is a vital transcription factor within the ATF/CREB family, playing a critical role in regulating gene expression in response to cAMP signaling pathways. The functionality of CREM is significantly influenced by post-translational modifications such as phosphorylation, which are essential for modulating transcriptional activity, stability, and interactions with other proteins. These modifications are crucial for CREM′s involvement in key biological processes, including spermatogenesis, where CREM activity is indispensable for male fertility, as evidenced by infertility in male mice lacking a functional CREM gene. Additionally, CREM interacts with tissue-specific partners like ACT (activator of CREM in the testis), whose activity is regulated by the testis-specific kinesin KIF17b, highlighting the importance of post-translational regulation in function. CREM′s ability to influence cellular differentiation, proliferation, and apoptosis underscores significance in maintaining cellular homeostasis and potential impact on various physiological and pathological conditions. CREM (C-2) antibody is also available in multiple conjugated forms, including agarose, horseradish peroxidase (HRP), phycoerythrin (PE), fluorescein isothiocyanate (FITC), and various Alexa Fluor® conjugates, providing versatility for diverse experimental needs.

Alexa Fluor^® is a trademark of Molecular Probes Inc., OR., USA

LI-COR^® and Odyssey^® are registered trademarks of LI-COR Biosciences

CREM Antibody (C-2) References:

1. Specialized rules of gene transcription in male germ cells: the CREM paradigm.  |  Monaco, L., et al. 2004. Int J Androl. 27: 322-7. PMID: 15595950
2. Genetic control of spermiogenesis: insights from the CREM gene and implications for human infertility.  |  Krausz, C. and Sassone-Corsi, P. 2005. Reprod Biomed Online. 10: 64-71. PMID: 15705296
3. CREM activator and repressor isoform expression in human male germ cells.  |  Blöcher, S., et al. 2005. Int J Androl. 28: 215-23. PMID: 16048633
4. Regulation of gene expression in post-meiotic male germ cells: CREM-signalling pathways and male fertility.  |  Hogeveen, KN. and Sassone-Corsi, P. 2006. Hum Fertil (Camb). 9: 73-9. PMID: 16825108
5. Transcription factor ATF cDNA clones: an extensive family of leucine zipper proteins able to selectively form DNA-binding heterodimers.  |  Hai, TW., et al. 1989. Genes Dev. 3: 2083-90. PMID: 2516827
6. Interaction of a common cellular transcription factor, ATF, with regulatory elements in both E1a- and cyclic AMP-inducible promoters.  |  Lin, YS. and Green, MR. 1988. Proc Natl Acad Sci U S A. 85: 3396-400. PMID: 2835770
7. Identification of a cyclic-AMP-responsive element within the rat somatostatin gene.  |  Montminy, MR., et al. 1986. Proc Natl Acad Sci U S A. 83: 6682-6. PMID: 2875459
8. Quercetin upregulates CREM gene expression in cyanide-induced endocrine dysfunction.  |  Oyewopo, A., et al. 2021. Heliyon. 7: e06901. PMID: 34027151
9. Dual regulation of miR-375 and CREM genes in pancreatic beta cells.  |  Keller, DM. and Perez, IG. 2022. Islets. 14: 139-148. PMID: 35377267
10. The oncogenic properties of the EWSR1::CREM fusion gene are associated with polyamine metabolism.  |  Kaprio, H., et al. 2023. Sci Rep. 13: 4884. PMID: 36966162
11. MITF::CREM-rearranged tumor: a novel group of cutaneous tumors with melanocytic differentiation.  |  Kalmykova, A., et al. 2023. Virchows Arch. 483: 569-575. PMID: 37550584
12. Regulating the balance between differentiation and apoptosis: role of CREM in the male germ cells.  |  Sassone-Corsi, P. 1998. J Mol Med (Berl). 76: 811-7. PMID: 9846951

Monoclonal antibodies for mammalian and non-mammalian protein targets represent essentially all targets covered by polyclonal antibodies. Over 8,300 of our antibodies are offered as ImmunoCruz^® conjugates which eliminates the need for a secondary antibody.

Santa Cruz Biotechnology antibodies have over 360,000 research citations. Anti-CREM Antibody (C-2) has 8 citations in a variety of scientific publications.

Primary antibodies like Anti-CREM Antibody (C-2) for mammalian target proteins are recommended for the detection of a range of mammalian species, primarily of mouse, rat and human species. Many mammalian antibodies are also ideal for veterinary research and reactive with ovine, porcine, caprine, feline, canine, bovine and equine protein targets. Santa Cruz Biotechnology primary monoclonal antibodies are suitable for laboratory research applications and are not intended for diagnostic or therapeutic use.

CREM Antibody (C-2) Specifications

Alternate Product Name: CREM

Product Clone Name: C-2

Classification: Primary Antibody

Clonality: Monoclonal Antibody

Antibody Isotype: IgG_2a kappa light chain

Amino Acids: 1-153

Epitope Target: CREM

Epitope Species: mouse

Product Reactivity/Detection: CREM

Species Reactivity/Detection: mouse, rat, human

Additional Mammalian Reactivity: N/A

Additional Non-Mammalian Reactivity: N/A

Applications: WB, IP, IF, IHC(P), ELISA

Vialing Information: Each vial contains 200 µg IgG_2a kappa light chain in 1.0 ml of PBS with < 0.1% sodium azide and 0.1% gelatin

Research Citations: 8 citations in various scientific publications

Available Conjugates/Forms for CREM Antibody (C-2): AC: Agarose, AF488: Alexa Fluor^® 488, AF546: Alexa Fluor^® 546, AF594: Alexa Fluor^® 594, AF647: Alexa Fluor^® 647, AF680: Alexa Fluor^® 680, AF790: Alexa Fluor^® 790, FITC: Fluoroscein, HRP: Horseradish Peroxidase, PE: Phycoerythrin

The preferred CREB1 antibody is CREB1 Antibody (D-12): sc-377154.