Date published: 2025-9-6
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cPLA<sub>2</sub> Antibody (4-4B-3C) - Western Blotting - Image 354723
cPLA2 抗体 (4-4B-3C): sc-454. RPMI2650 (A), RAW 264. 7 (B), 3T3-L1 (C) 和 NRK (D) 全细胞裂解液中 cPLA2 表达的 Western 印迹分析.
cPLA2 抗体 (4-4B-3C): sc-454. RPMI2650 (A), RAW 264. 7 (B), 3T3-L1 (C) 和 NRK (D) 全细胞裂解液中 cPLA2 表达的 Western 印迹分析.

cPLA2 抗体 (4-4B-3C): sc-454

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说明书
  • cPLA2抗体(4-4B-3C)是小鼠单克隆IgG2b κ, 在135篇文献中引用，规格为200 µg/ml
  • 免疫human来源cPLA2位于N-terminus 的1-216氨基酸
  • 抗-cPLA2 抗体 (4-4B-3C) 推荐用于 WB, IP, IF, IHC(P) 和 FCM，检测mouse, rat 和human 来源的 cytosolic PLA2
  • 抗cPLA2抗体（4-4B-3C）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取cPLA2 (4-4B-3C): sc-454的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG2b BP-HRP 和 m-IgGκ BP-HRP 是 cPLA2 抗体 (4-4B-3C) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与cPLA2 抗体 (4-4B-3C) 组合成套装提供。 (请参阅下面的订购信息)。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

cPLA 抗体

cPLA2抗体（4-4B-3C）是一种小鼠单克隆IgG2b kappa轻链抗体，可通过多种应用检测小鼠、大鼠和人类样本中的细胞质磷脂酶A2（cPLA2），包括蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF 、免疫组化以及流式细胞术（FCM）等多种应用中检测小鼠、大鼠和人类样本中的细胞质磷脂酶A2（cPLA2）。cPLA2单克隆抗体（4-4B-3C）有非缀合和多种缀合形式，如琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异 异氰酸酯（FITC）以及各种Alexa Fluor®偶联物，可满足不同实验需求。cPLA2通过水解甘油磷脂中的sn-2-酰基酯键，从而释放出包括花生四烯酸在内的游离脂肪酸，在脂质代谢中发挥着至关重要的作用。这个过程至关重要，因为花生四烯酸是合成前列腺素和白三烯等类花生酸的前体，而前列腺素和白三烯是炎症反应的关键介质。cPLA2能够从膜磷脂中优先释放花生四烯酸，这凸显了其在调节炎症过程和细胞信号通路中的重要性。cPLA2 (4-4B-3C)抗体使研究人员能够有效地研究cPLA2在各种生物学环境中的动态变化，从而加深我们对其在健康和疾病中的作用的理解。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

cPLA2 抗体 (4-4B-3C) 参考文献:

  1. 细胞外磷脂酶 A2 活性的调节：对炎症性疾病的影响。  |  Mukherjee, AB., et al. 1992. DNA Cell Biol. 11: 233-43. PMID: 1567556
  2. 人 Ca(2+) 敏感性细胞膜磷脂酶 A2 的分子克隆和表达。  |  Sharp, JD., et al. 1991. J Biol Chem. 266: 14850-3. PMID: 1869522
  3. 磷脂酶 A2 表面识别的新型双功能机制。  |  Heinrikson, RL. and Kézdy, FJ. 1990. Adv Exp Med Biol. 279: 37-47. PMID: 2096699
  4. 从人类单核细胞白血病 U937 细胞中纯化磷脂酶 A2。钙依赖性激活和膜结合。  |  Diez, E. and Mong, S. 1990. J Biol Chem. 265: 14654-61. PMID: 2201689
  5. 从人类单核细胞系 U937 中纯化出 110 千道尔顿的细胞膜磷脂酶 A2。  |  Clark, JD., et al. 1990. Proc Natl Acad Sci U S A. 87: 7708-12. PMID: 2217203
  6. PLA2G4A/cPLA2- 介导的溶酶体膜损伤导致自噬抑制和脑外伤后的神经退行性变。  |  Sarkar, C., et al. 2020. Autophagy. 16: 466-485. PMID: 31238788
  7. 巨噬细胞系 RAW 264.7 中花生四烯酸水解磷脂酶 A2 的特性和纯化。  |  Leslie, CC., et al. 1988. Biochim Biophys Acta. 963: 476-92. PMID: 3143418
  8. 通过 cPLA2-MAVS 对星形胶质细胞致病活性的代谢控制  |  Chao, CC., et al. 2019. Cell. 179: 1483-1498.e22. PMID: 31813625
  9. 抑制PLA2G4E/cPLA2通过减轻溶酶体膜通透性诱导的坏死，促进随机皮肤皮瓣的存活。  |  Lou, J., et al. 2022. Autophagy. 18: 1841-1863. PMID: 34872436
  10. HDAC5 缺失会增强磷脂衍生的花生四烯酸的生成并使胰腺癌对 cPLA2 抑制更敏感  |  Pan, P., et al. 2022. Cancer Res. 82: 4542-4554. PMID: 36102738
  11. 来自 Crotalus adamanteus 毒液的磷脂酶 A2-α 的氨基酸序列。基于结构决定因素的磷脂酶 A2 新分类。  |  Heinrikson, RL., et al. 1977. J Biol Chem. 252: 4913-21. PMID: 873920

订购信息

产品名称产品编号规格价格数量收藏夹

cPLA2 抗体 (4-4B-3C)

sc-454
200 µg/ml
$316.00

cPLA2 (4-4B-3C): m-IgGκ BP-HRP 套装

sc-520410
200 µg Ab, 40 µg BP
$354.00

cPLA2 (4-4B-3C): m-IgG2b BP-HRP 套装

sc-548826
200 µg Ab; 10 µg BP
$354.00

cPLA2 抗体 (4-4B-3C) AC

sc-454 AC
500 µg/ml, 25% agarose
$416.00

cPLA2 抗体 (4-4B-3C) HRP

sc-454 HRP
200 µg/ml
$316.00

cPLA2 抗体 (4-4B-3C) FITC

sc-454 FITC
200 µg/ml
$330.00

cPLA2 抗体 (4-4B-3C) PE

sc-454 PE
200 µg/ml
$343.00

cPLA2 抗体 (4-4B-3C) Alexa Fluor® 488

sc-454 AF488
200 µg/ml
$357.00

cPLA2 抗体 (4-4B-3C) Alexa Fluor® 546

sc-454 AF546
200 µg/ml
$357.00

cPLA2 抗体 (4-4B-3C) Alexa Fluor® 594

sc-454 AF594
200 µg/ml
$357.00

cPLA2 抗体 (4-4B-3C) Alexa Fluor® 647

sc-454 AF647
200 µg/ml
$357.00

cPLA2 抗体 (4-4B-3C) Alexa Fluor® 680

sc-454 AF680
200 µg/ml
$357.00

cPLA2 抗体 (4-4B-3C) Alexa Fluor® 790

sc-454 AF790
200 µg/ml
$357.00

Is this antibody specific for cPLA2 alpha? One of the publications listed here (Biochemical and Biophysical Research Communications Volume 496, Issue 1, 29 January 2018, Pages 153-158) uses it to detect cPLA2 alpha.

Asked by: AlanDB
Thank you for your question. It would be helpful if you could call 800-457-3801, allowing for a more interactive discussion of this and other related questions.
Answered by: Technical Service
Date published: 2023-03-01

Buenas tardes, cu&aacute;l es la diluci&oacute;n recomendada para este anticuerpo con el objetivo de realizar inmunofluorescencia en cultivo primario de astrocitos de rata? Muchas gracias.

Asked by: AlejandrocPLA2
Gracias por su pregunta. El rango de diluci&oacute;n recomendado para inmunofluorescencia es 1:50 - 1:100. Thank you for your question. The recommended dilution range for immunofluorescence is 1:50 - 1:100.
Answered by: Tech Support Europe
Date published: 2021-01-25

HI, I am a graduate student in the at University of Michigan. I am interested in buying the cPLA2 antibody (catalog#sc-454 and sc-376618). Would it be possible to get a sample of the antibody to test it out? Please advise. Sincerely, Fatima Javed

Asked by: javed
Thank you for your question. Please contact Technical Service for information on how to obtain a sample.
Answered by: Technical Support
Date published: 2018-12-14

Can cPLA2 Antibody (4-4B-3C): sc-454 be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: sweetums
Yes, cPLA2 Antibody (4-4B-3C): sc-454 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support 13
Date published: 2017-01-12

Will this monoclonal antibody detect the bovine cPLA2 protein?

Asked by: sweetums
Please contact our Technical Service Department and we would be happy to perform sequence analysis and review our data to answer any questions you may have about additional species reactivity.
Answered by: Technical Support 13
Date published: 2017-01-12
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Rated 5 out of 5 by from Fabulous features!We use this antibody to characterize the features of cPLA2 in mouse preadipocytes, and the effect is very good.
Date published: 2021-07-08
Rated 5 out of 5 by from The primary antibody is good.The primary antibody is available for IHC in human cancer sample.
Date published: 2020-06-08
Rated 5 out of 5 by from works great for immunoflouresenceworks great for immunoflouresence for murine DCs. recommended.
Date published: 2020-03-12
Rated 5 out of 5 by from This antibody is excellentWe use this antibody to characterize the spatial features of cPLA2 in tumor tissue, and the effect is very good.
Date published: 2019-11-05
Rated 5 out of 5 by from Worked great on WBI bought the trial version few weeks back and it work great on detecting cPLA2 over-expression in my transfected cell line.
Date published: 2018-01-30
Rated 4 out of 5 by from Nice primary antibodyThe primary antibody is available for Western blotting in mice sample.
Date published: 2017-06-13
Rated 4 out of 5 by from Works pretty wellI tried this antibody on mouse brain NP40 homogenates. I saw an intense band at ~115 kDa and a weaker band above it (phospho-cPLA2). I also saw a couple of additional weak bands (a 50 kDa and 35 kDa). I also tried it for mouse IHC using PFA-fixed coronal brain sections but did not detect any signal.
Date published: 2017-05-01
Rated 5 out of 5 by from WB: Worked well in mouse brain tissue samplesGreat WB signal obtained using this antibody sample at 1:500 dilultion against mouse brain tissue lysates. Clear band around 100kD and better than abcam ab58375. Good and quick service. Thank you.
Date published: 2017-04-17
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cPLA 2 抗体 (4-4B-3C) is rated 4.7 out of 5 by 20.
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