CEACAM1抗体 (E-1) 推荐用于 human 来源的CEACAM1 WB, IP, IF, IHC(P) 和 ELISA检测

CEACAM1 抗体 (E-1): sc-166453

CEACAM1抗体 (E-1) is rated 4.8 out of 5 by 5.
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说明书

    • 抗-CEACAM1 抗体 E-1 是小鼠单克隆 IgG2b κ,CEACAM1抗体, 在1篇文献中引用,规格为200 µg/ml
    • raised against amino acids 391-526 mapping at the C-terminus of CEACAM1 of human origin
    • CEACAM1 抗体 (E-1) 推荐用于WB, IP, IF, IHC(P) 和 ELISA,检测human 来源的 CEACAM1
    • 抗-CEACAM1 抗体 (E-1) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 关于如何获取CEACAM1 (E-1): sc-166453免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) is the preferred secondary detection reagent for CEACAM1 Antibody (E-1) for WB and IHC(P) applications. This reagent is now offered in a bundle with CEACAM1 Antibody (E-1) (see ordering information below). For additional m-IgGκ BP conjugates see our complete list of Mouse IgG Binding Proteins.

What is the recommended fixation method for immunofluorescence staining with CEACAM1 (E-1): sc-166453 monoclonal antibody?

Asked by: Cweed
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 5 out of 5 by from Strong band in WB I was interested in evaluating the expression of CEACAM-1 in whole lysates of primary human colon cancer stem cells (CSC. 25 micrograms/lane). The E1 mAb yielded a very strong, broad band in the 60-80 kD range (dilution 1:200 in 3% skim milk, incubation O/n at 4°C). The companion antibody GM8G5 (sc-59812) gave no signal (see accompanying picture)
Date published: 2020-10-27
Rated 5 out of 5 by from Positive results in WB Band for several epithelial cell lines in whole cell lysates
Date published: 2017-11-24
Rated 4 out of 5 by from Positive results in western blot analysis Positive results in western blot analysis of CEACAM1 expression in SW480 and T84 whole cell lysates. -SCBT QC
Date published: 2015-04-18
Rated 5 out of 5 by from WB: robust band in Hep G2 whole cell lysate WB: robust band in Hep G2 whole cell lysate. -SCBT QC
Date published: 2015-04-13
Rated 5 out of 5 by from Superb immunofluorescence intracellular staining Superb immunofluorescence intracellular staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-01-23
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