Date published: 2025-9-7

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Cdc34 抗体 (H-4): sc-28381

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说明书
  • Cdc34 抗体 H-4 是小鼠单克隆 IgG2a κ,Cdc34抗体, 在2篇文献中引用,规格为200 µg/ml
  • 免疫human物种的Cdc34的氨基酸144-224
  • Cdc34 抗体 (H-4) 推荐用于 WB, IP, IF, IHC(P) 和 ELISA,检测mouse, rat 和human 来源的 Cdc34
  • 抗Cdc34抗体(H-4)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取Cdc34 (H-4): sc-28381免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP, m-IgG2a BP-HRPm-IgGκ BP-HRP 是 Cdc34 抗体 (H-4) 在 WB and IHC(P) 应用中的首选二抗检测试剂。这些试剂现与Cdc34 抗体 (H-4) 组合成套装提供。 (请参阅下面的订购信息)。

快捷链接

関連項目

Cdc34抗体(H-4)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)、免疫组织化学和酶联免疫吸附测定(ELISA )。抗Cdc34抗体(H-4)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种Alexa Fluor®结合物。Cdc34 (H-4) 抗体可识别一种在细胞周期中发挥关键作用的蛋白质,该蛋白质作为泛素结合酶,对 G1 到 S 期的过渡至关重要。这一过程对于正常的细胞分裂和增殖至关重要,因为Cdc34可调节细胞周期蛋白的降解和细胞周期蛋白依赖性激酶(Cdks)的激活。Cdc34 (H-4) 单克隆抗体靶向一种参与细胞周期蛋白B泛素化的蛋白质,后者是一种关键调节因子,当其降解时,细胞可从有丝分裂进入细胞周期的下一阶段。Cdc34与其它蛋白(如Cdc27和Cdc16)的相互作用进一步强调了Cdc34在维持细胞周期完整性和防止细胞无序生长方面的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

Cdc34 抗体 (H-4) 参考文献:

  1. 蛋白激酶 CK2 介导的 Cdc34 磷酸化控制蛋氨酸生物合成基因。  |  Barz, T., et al. 2006. Cell Mol Life Sci. 63: 2183-90. PMID: 16952051
  2. 泛素-蛋白酶体系统是决定对甲基汞敏感性的一个因素。  |  Hwang, GW. 2007. Yakugaku Zasshi. 127: 463-8. PMID: 17329932
  3. cdc25 是一种特异性酪氨酸磷酸酶,可直接激活 p34cdc2。  |  Gautier, J., et al. 1991. Cell. 67: 197-211. PMID: 1913817
  4. 酵母(Saccharomyces cerevisiae)cdc突变体中人工染色体的有丝分裂传递。  |  Palmer, RE., et al. 1990. Genetics. 125: 763-74. PMID: 2204582
  5. 细胞周期的新转折  |  Barinaga, M. 1995. Science. 269: 631-2. PMID: 7624789
  6. 含有 CDC27 和 CDC16 的 20S 复合物可催化泛素与细胞周期蛋白 B 的有丝分裂特异性连接。  |  King, RW., et al. 1995. Cell. 81: 279-88. PMID: 7736580
  7. 通过酵母互补克隆出 CDC34 细胞周期基因的人类同源物。  |  Plon, SE., et al. 1993. Proc Natl Acad Sci U S A. 90: 10484-8. PMID: 8248134
  8. 哺乳动物的 p50Cdc37 是 Hsp90 的蛋白激酶靶向亚基,能与 Cdk4 结合并使其稳定。  |  Stepanova, L., et al. 1996. Genes Dev. 10: 1491-502. PMID: 8666233
  9. 一种与酵母 Cdc6p 相关的人类蛋白质。  |  Williams, RS., et al. 1997. Proc Natl Acad Sci U S A. 94: 142-7. PMID: 8990175

订购信息

产品名称产品编号规格价格数量收藏夹

Cdc34 抗体 (H-4)

sc-28381
200 µg/ml
$316.00

Cdc34 (H-4): m-IgG Fc BP-HRP 套装

sc-528439
200 µg Ab; 10 µg BP
$354.00

Cdc34 (H-4): m-IgGκ BP-HRP 套装

sc-520817
200 µg Ab, 40 µg BP
$354.00

Cdc34 (H-4): m-IgG2a BP-HRP 套装

sc-547099
200 µg Ab; 10 µg BP
$354.00

Cdc34 抗体 (H-4) AC

sc-28381 AC
500 µg/ml, 25% agarose
$416.00

Cdc34 抗体 (H-4) HRP

sc-28381 HRP
200 µg/ml
$316.00

Cdc34 抗体 (H-4) FITC

sc-28381 FITC
200 µg/ml
$330.00

Cdc34 抗体 (H-4) PE

sc-28381 PE
200 µg/ml
$343.00

Cdc34 抗体 (H-4) Alexa Fluor® 488

sc-28381 AF488
200 µg/ml
$357.00

Cdc34 抗体 (H-4) Alexa Fluor® 546

sc-28381 AF546
200 µg/ml
$357.00

Cdc34 抗体 (H-4) Alexa Fluor® 594

sc-28381 AF594
200 µg/ml
$357.00

Cdc34 抗体 (H-4) Alexa Fluor® 647

sc-28381 AF647
200 µg/ml
$357.00

Cdc34 抗体 (H-4) Alexa Fluor® 680

sc-28381 AF680
200 µg/ml
$357.00

Cdc34 抗体 (H-4) Alexa Fluor® 790

sc-28381 AF790
200 µg/ml
$357.00

Can Cdc34 (H-4): sc-28381 monoclonal antibody be used to stain formalin-fixed, paraffin-embedded (FFPE) tissue sections?

Asked by: Cweed
Thank you for your question. Yes, Cdc34 (H-4): sc-28381 has been validated for use in IHC with paraffin-embedded sections. We recommend performing antigen retrieval with sodium citrate buffer (pH 6) and heat. The full protocol can be found here: https://www.scbt.com/scbt/resources/protocols/immunoperoxidase-staining
Answered by: Technical Support
Date published: 2017-02-27
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Rated 5 out of 5 by from ottimo funzionamentosono molto soddisfatto. Usato per Western blot diluito 1: 1000 in TBST + 5%BSA
Date published: 2023-12-06
Rated 4 out of 5 by from Potent and fairly specific antibodyThis monoclonal antibody was independently tested in-house by Kinexus Bioinformatics Corporation and validated for use for Western blotting. There was medium detection of the ~34 kDa target protein in mouse tissues (brain/testes/thymus/muscle mix). No major cross-reactivities were observed at 2 µg/ml antibody concentration with off-target proteins in the mouse tissues tested, but a higher background and some weak cross-reactive proteins were evident.
Date published: 2016-11-10
Rated 5 out of 5 by from Worked with human transfected lysate by WBWorked with human transfected lysate by WB -SCBT QC
Date published: 2015-04-25
Rated 5 out of 5 by from Good specificity and signal strength forGood specificity and signal strength for WB in HeLa whole cell lysate and HeLa, K-562 and A-431 nuclear extracts. -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Worked with mouse transfected lysates by WBWorked with mouse transfected lysates by WB -SCBT QC
Date published: 2015-01-20
Rated 5 out of 5 by from Robust nuclear and cytoplasmic staining inRobust nuclear and cytoplasmic staining in paraffin-embedded human small intestine. -SCBT QC
Date published: 2014-05-04
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Cdc34 抗体 (H-4) is rated 4.8 out of 5 by 6.
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