Date published: 2025-9-6
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CD8 Antibody (32-M4) - Western Blotting - Image 284200
CD8 抗体 (32-M4): sc-1177. CCRF-CEM 全细胞裂解液中 CD8 表达的 Western 印迹分析.
CD8 抗体 (32-M4): sc-1177. CCRF-CEM 全细胞裂解液中 CD8 表达的 Western 印迹分析.

CD8 抗体 (32-M4): sc-1177

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寫評論提問

说明书
  • CD8 抗体 32-M4 是小鼠单克隆 IgG2a κ，CD8抗体, 在31篇文献中引用，规格为200 µg/ml
  • 免疫human物种的CD8
  • CD8 抗体 (32-M4) 推荐用于 WB, IP, IF 和 FCM，检测mouse, rat 和human 来源的 CD8
  • 抗CD8抗体（32-M4）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 还可偶联APC 用于IF, IHC(P) 和FCM
  • 关于如何获取CD8 (32-M4): sc-1177的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD8 抗体 (32-M4)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

此外 CD8 抗体包括 CD8-α 和 CD8-β

CD8抗体（32-M4）是一种小鼠单克隆IgG2a kappa轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测小鼠、大鼠和人类来源的CD8蛋白。抗CD8抗体（32-M4）有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶（HRP）、藻红蛋白（PE）、异硫氰酸荧光素（FITC）和多种Alexa Fluor®结合物。CD8蛋白，也称为Leu 2或T8，是一种重要的细胞表面糖蛋白，可形成双链复合物（αα或αβ），通过与抗原呈递细胞（APC）呈现的I类主要组织相容性复合体（MHC）分子结合，在免疫反应中发挥重要作用。这种相互作用对于激活T细胞毒性细胞至关重要，而T细胞毒性细胞负责直接杀死受感染或癌变的细胞。CD8可增强T细胞受体（TCR）与抗原相互作用的亲和力，从而促进有效的免疫反应。表达CD8的T细胞有助于维持低水平的HIV表达，这凸显了CD8在适应性免疫和病毒控制中的重要性。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD8 抗体 (32-M4) 参考文献:

  1. 通过调节胆固醇代谢增强CD8(+) T细胞的抗肿瘤反应。  |  Yang, W., et al. 2016. Nature. 531: 651-5. PMID: 26982734
  2. CD8+ T细胞分化的表观遗传控制。  |  Henning, AN., et al. 2018. Nat Rev Immunol. 18: 340-356. PMID: 29379213
  3. 胆固醇诱导肿瘤微环境中的CD8+ T细胞衰竭。  |  Ma, X., et al. 2019. Cell Metab. 30: 143-156.e5. PMID: 31031094
  4. 转录物和表观遗传学调控CD8+ T细胞衰竭。  |  Khan, O., et al. 2019. Nature. 571: 211-218. PMID: 31207603
  5. CD8+ T细胞亚群在抗肿瘤免疫中的作用。  |  St Paul, M. and Ohashi, PS. 2020. Trends Cell Biol. 30: 695-704. PMID: 32624246
  6. 记忆性CD8+ T细胞对癌症的反应。  |  Han, J., et al. 2020. Semin Immunol. 49: 101435. PMID: 33272898
  7. 全身疫苗接种诱导CD8+ T细胞并重塑肿瘤微环境。  |  Baharom, F., et al. 2022. Cell. 185: 4317-4332.e15. PMID: 36302380
  8. CD8+ T细胞衰竭与癌症免疫疗法。  |  Wang, Q., et al. 2023. Cancer Lett. 559: 216043. PMID: 36584935
  9. mTOR信号对CD8+ T细胞记忆和衰竭的调控。  |  Chen, Y., et al. 2023. Cell Mol Immunol. 20: 1023-1039. PMID: 37582972
  10. CD8+ T细胞的昼夜肿瘤浸润和功能决定免疫疗法的疗效。  |  Wang, C., et al. 2024. Cell. 187: 2690-2702.e17. PMID: 38723627

订购信息

产品名称产品编号规格价格数量收藏夹

CD8 抗体 (32-M4)

sc-1177
200 µg/ml
$316.00

CD8 抗体 (32-M4) AC

sc-1177 AC
500 µg/ml, 25% agarose
$416.00

CD8 抗体 (32-M4) HRP

sc-1177 HRP
200 µg/ml
$316.00

CD8 抗体 (32-M4) FITC

sc-1177 FITC
200 µg/ml
$330.00

CD8 抗体 (32-M4) PE

sc-1177 PE
200 µg/ml
$343.00

CD8 抗体 (32-M4) Alexa Fluor® 488

sc-1177 AF488
200 µg/ml
$357.00

CD8 抗体 (32-M4) Alexa Fluor® 546

sc-1177 AF546
200 µg/ml
$357.00

CD8 抗体 (32-M4) Alexa Fluor® 594

sc-1177 AF594
200 µg/ml
$357.00

CD8 抗体 (32-M4) Alexa Fluor® 647

sc-1177 AF647
200 µg/ml
$357.00

CD8 抗体 (32-M4) Alexa Fluor® 680

sc-1177 AF680
200 µg/ml
$357.00

CD8 抗体 (32-M4) Alexa Fluor® 790

sc-1177 AF790
200 µg/ml
$357.00

CD8 抗体 (32-M4) APC

sc-1177 APC
100 tests in 2 ml
$351.00

can it be used to assess the immunohistochemical expression of CD8 in visceral leishmaniasis?

Asked by: ban jasim
Thank you for your question. No, not to our knowledge. This antibody is recommended for detection of CD8 of mouse, rat and human origin.
Answered by: Technical Support
Date published: 2018-12-10

Does this antibody recognize alpha or beta chain of CD8? Thank you for your reply.

Asked by: Matea
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody, CD8 (32-M4): sc-1177, has not yet been determined. And therefore we do not know if the antibody is specific for the alpha or beta chain of CD8.
Answered by: Technical Support
Date published: 2017-07-18

If I want to use this antibody for Flow Cytometry, what starting dilution do you recommend I use?

Asked by: TinTin
We recommend a starting dilution of 1µg of antibody per million cells. Here is a link to our recommended protocol. https://www.scbt.com/scbt/resources/protocols/flow-cytometry
Answered by: Tech Service
Date published: 2017-01-27
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Rated 1 out of 5 by from Didn't work for WB under the conditions I usedUnfortunately this antibody did not work on my WB.
Date published: 2018-02-13
Rated 5 out of 5 by from nice antibodywork well in WB experiment and IHC experiment , the band is very clear
Date published: 2018-01-08
Rated 4 out of 5 by from Great features!The antibody is work well in WB at 1:100,skeletal muscle cells.
Date published: 2017-09-01
Rated 5 out of 5 by from Well characterized CD8 monoclonal ab for IF & IHCLots of citations in primary literature for variety of applications, WB, IP, IF and IHC on human and mouse samples. Stong clean band for WB on HuT 78 and CCRF-CEM whole cell lysate. Clean FCM results in the third decade for SUP-T1 cells
Date published: 2016-12-23
Rated 4 out of 5 by from Great FCM data of SUPGreat FCM data of SUP-T1 cells. -SCBT QC
Date published: 2015-06-01
Rated 4 out of 5 by from Publishable data in IHC using mouse lungPublishable data in IHC using mouse lung cell lines. -SCBT Publication Review
Date published: 2015-05-19
Rated 4 out of 5 by from Various publishable data in IF using humanVarious publishable data in IF using human monocytes and t cells. -SCBT Publication Review
Date published: 2015-05-03
Rated 4 out of 5 by from Band of interest seen in WB using HuT 78Band of interest seen in WB using HuT 78 whole cell lysate. -SCBT QC
Date published: 2015-04-06
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CD8 抗体 (32-M4) is rated 3.9 out of 5 by 11.
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