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CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1) - Western Blotting - Image 9570
CD71 抗体 (3B8 2A1): sc-32272. CCRF-CEM (A), Jurkat (B), PMA 处理的 Jurkat (C), K-562 (D) 和 IL-2 处理的 Jurkat (E) 全细胞裂解液中 CD71 表达的 Western 印迹分析.
CD71 抗体 (3B8 2A1): sc-32272. CCRF-CEM (A), Jurkat (B), PMA 处理的 Jurkat (C), K-562 (D) 和 IL-2 处理的 Jurkat (E) 全细胞裂解液中 CD71 表达的 Western 印迹分析.

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1): sc-32272

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寫評論提問

说明书
  • CD71/TFRC/Transferrin Receptor 抗体 3B8 2A1 是小鼠单克隆 IgG1 κ，CD71/TFRC/Transferrin Receptor抗体, 在66篇文献中引用，规格为200 µg/ml
  • 针对human CD71的纯化外显子域
  • CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) 推荐用于WB, IP, IF 和 FCM，检测human 来源的 CD71
  • 抗CD71/TFRC/Transferrin Receptor抗体（3B8 2A1）可与琼脂糖结合用于IP；与HRP结合用于WB、IHC(P)和ELISA；与藻红蛋白或FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594 和 Alexa Fluor® 647，用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统，例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680 和 Alexa Fluor® 790， 用于WB (NIR), IF 和 FCM; 以及用于近红外（NIR）检测系统，如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CD71/TFRC/Transferrin Receptor (3B8 2A1): sc-32272的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP和 1 BP-HRP">m-IgG1 BP-HRP是CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1) for WB applications. 的首选辅助检测试剂，这些试剂现与CD71/TFRC/Transferrin Receptor Antibody (3B8 2A1) 打包提供（请参阅下面的订购信息）。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD71抗体（3B8 2A1）是一种小鼠单克隆IgG1κ轻链抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）和流式细胞术（FCM）检测人类来源的CD71蛋白。CD71 (3B8 2A1) 抗体有非结合型和多种结合型，包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 结合物。CD71蛋白，也称为转铁蛋白受体（TFR），是一种II型膜糖蛋白，通过受体介导的内吞作用，调节细胞对转铁蛋白结合铁的摄取，在铁稳态中发挥关键作用。这一过程对于维持充足的铁含量至关重要，而充足的铁含量对于各种细胞功能（包括氧气运输和DNA合成）必不可少。CD71通常在增殖细胞、网状细胞和红细胞前体中大量表达，而在活化的淋巴细胞、单核细胞和巨噬细胞中表达上调。CD71也存在于大多数分裂细胞和脑内皮细胞中，这凸显了CD71在造血和神经生物学中的重要性。此外，第二种转铁蛋白受体TFR2也有助于摄取转铁蛋白结合的铁，主要在肝脏中表达，TFR2基因突变可导致遗传性血色素沉着病III型，这是一种铁超负荷疾病。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) 参考文献:

  1. 转铁蛋白受体对免疫突触的招募是对 TCR 参与的反应。  |  Batista, A., et al. 2004. J Immunol. 172: 6709-14. PMID: 15153487
  2. 树突状细胞成熟过程中 IgA 受体（CD89 和 CD71）的表达和功能差异。  |  Pasquier, B., et al. 2004. J Leukoc Biol. 76: 1134-41. PMID: 15371488
  3. 用抗转铁蛋白受体马巴抑制淋巴细胞活化：三种试剂的比较及其作用范围和作用机制的进一步研究。  |  Kemp, JD., et al. 1989. Cell Immunol. 122: 218-30. PMID: 2526689
  4. 转铁蛋白受体在淋巴细胞生长中的作用：针对小鼠转铁蛋白受体的大鼠 IgG 单克隆抗体可对 T 细胞和 B 细胞活化协议产生高度选择性抑制。  |  Kemp, JD., et al. 1987. J Immunol. 138: 2422-6. PMID: 2951439
  5. 单克隆抗转铁蛋白受体抗体抑制细胞生长。  |  Lesley, JF. and Schulte, RJ. 1985. Mol Cell Biol. 5: 1814-21. PMID: 3018527
  6. ESR1 通过 NEDD4L/CD71 通路抑制电离辐射诱导的乳腺癌细胞铁变态反应。  |  Liu, L., et al. 2022. Arch Biochem Biophys. 725: 109299. PMID: 35613689
  7. 阻断转铁蛋白受体功能的单克隆抗体对合成小鼠白血病体内生长的影响  |  Sauvage, CA., et al. 1987. Cancer Res. 47: 747-53. PMID: 3802079
  8. CD71表达的循环中性粒细胞可作为胰腺导管腺癌患者转移扩散和预后不良的新生物标志物。  |  Hansen, FJ., et al. 2024. Sci Rep. 14: 21164. PMID: 39256468
  9. 人类转铁蛋白受体基因：基因组组织以及从 cDNA 序列推导出的受体完整一级结构。  |  McClelland, A., et al. 1984. Cell. 39: 267-74. PMID: 6094009
  10. 转铁蛋白受体在小鼠造血祖细胞上的表达。  |  Lesley, J., et al. 1984. Cell Immunol. 83: 14-25. PMID: 6319033
  11. 转铁蛋白受体表达作为小鼠未成熟周期性胸腺细胞的标志物。  |  Brekelmans, P., et al. 1994. Cell Immunol. 159: 331-9. PMID: 7994765
  12. 转铁蛋白受体  |  Testa, U., et al. 1993. Crit Rev Oncog. 4: 241-76. PMID: 8485201

订购信息

产品名称产品编号规格价格数量收藏夹

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1)

sc-32272
200 µg/ml
$316.00

CD71/TFRC/Transferrin Receptor (3B8 2A1): m-IgG Fc BP-HRP 套装

sc-526671
200 µg Ab; 10 µg BP
$354.00

CD71/TFRC/Transferrin Receptor (3B8 2A1): m-IgG1 BP-HRP 套装

sc-532044
200 µg Ab; 20 µg BP
$354.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) AC

sc-32272 AC
500 µg/ml, 25% agarose
$416.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) HRP

sc-32272 HRP
200 µg/ml
$316.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) FITC

sc-32272 FITC
200 µg/ml
$330.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) PE

sc-32272 PE
200 µg/ml
$343.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) Alexa Fluor® 488

sc-32272 AF488
200 µg/ml
$357.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) Alexa Fluor® 546

sc-32272 AF546
200 µg/ml
$357.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) Alexa Fluor® 594

sc-32272 AF594
200 µg/ml
$357.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) Alexa Fluor® 647

sc-32272 AF647
200 µg/ml
$357.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) Alexa Fluor® 680

sc-32272 AF680
200 µg/ml
$357.00

CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) Alexa Fluor® 790

sc-32272 AF790
200 µg/ml
$357.00

Does this antibody [(3B8 2A1), sc-32272] block binding of Transferrin to CD71?

Asked by: PRCH
Thank you for your question. This antibody has not been tested for functional or blocking activity.
Answered by: Tech Service
Date published: 2019-11-04

What is the recommended fixation method for IF with CD71 Antibody (3B8 2A1): sc-32272?

Asked by: jenni
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10ºC methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support 3
Date published: 2017-01-09
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Rated 5 out of 5 by from Reliable productThe Hela cell lysate worked as described is perfect as a control for western blots.
Date published: 2021-05-24
Rated 2 out of 5 by from It does not work on mouse cells.It does not work on mouse cells (A20), but it is fair, as Santa Cruz recommended this antibody for detection of CD71 of human origin. I just gave it a try anyway.
Date published: 2018-03-08
Rated 3 out of 5 by from Good Flow, Poor IFAntibody worked well for FC at 1ug/1 million cells. Seemed to show some surface staining of T cells via IF at a 1:100 dilution.
Date published: 2017-11-20
Rated 5 out of 5 by from Good FeatureUsed for IF on human tissue, clean signal, good antibody.
Date published: 2017-10-05
Rated 5 out of 5 by from WB for TFRCVery clear western blot data; strong signal with low background；test at 1:300
Date published: 2017-09-14
Rated 5 out of 5 by from good featuresuse in NB4 cells ,the trips is very abvious,thank you for your free sample
Date published: 2017-09-06
Rated 4 out of 5 by from 还可以我用的rat来源的细胞样品，此抗体有两三条条带，但是抗体效价可以，条带也清晰，推荐大家使用！！！！！
Date published: 2017-08-01
Rated 5 out of 5 by from TFRCThe TFRC Antibody works very well for Western blot applications, showing strong and clear banding with minimal background noise present
Date published: 2017-07-31
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CD71/TFRC/Transferrin Receptor 抗体 (3B8 2A1) is rated 4.6 out of 5 by 17.
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