Date published: 2025-9-8
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CD40 Antibody (HM40-3) - Western Blotting - Image 4821
CD40 抗体 (HM40-3): sc-20010. 小鼠外周血裂解液中 CD40 表达的 Western 印迹分析.
CD40 抗体 (HM40-3): sc-20010. 小鼠外周血裂解液中 CD40 表达的 Western 印迹分析.

CD40 抗体 (HM40-3): sc-20010

4.3(3)
寫評論提問

说明书
  • CD40 Antibody (HM40-3) is a Armenian hamster monoclonal IgM provided at 200 µg/ml
  • 针对（BALB/c x NZB）F1 mouse衍生的淋巴瘤WEHI-231细胞
  • 推荐用于 mouse 和 rat 来源的CD40 WB, IP, IF, IHC(P) 和 FCM检测
  • 还提供无叠氮化物版本，用于阻断配体结合和刺激 B 淋巴细胞，sc-20010 L，200 µg/0.1 ml
  • 关于如何获取CD40 (HM40-3): sc-20010的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成CD40 抗体 (HM40-3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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描述
基因信息
说明书与实验方案
研究信息

関連項目

CD40抗体（HM40-3）是一种仓鼠单克隆IgM抗体，可通过蛋白质印迹（WB）、免疫沉淀（IP）、免疫荧光（IF）、免疫组织化学和流式细胞术（FCM）检测小鼠和大鼠来源的CD40蛋白。抗CD40抗体（HM40-3）以非结合形式提供，为研究人员提供了研究CD40信号传导途径的多功能工具。CD40在免疫反应中起着至关重要的作用，特别是在B细胞的活化和分化中。CD40主要位于B细胞表面，与活化T细胞上表达的CD40配体（CD40L）相互作用，促进细胞与细胞之间的必要交流。这种相互作用对于B细胞活化至关重要，可导致克隆扩增和抗体产生。CD40参与免疫球蛋白类转换和IgE分泌，而免疫球蛋白类转换和IgE分泌是适应性免疫反应的关键过程，这凸显了CD40在免疫调节中的重要性。此外，CD40信号传导途径由下游效应分子（如CRAF）介导，CRAF是TRAF1和TRAF2等蛋白家族的一部分，这些蛋白家族因调控肿瘤坏死因子受体信号传导和激活NFκB转录因子而闻名。了解CD40的功能和相互作用对于阐明免疫反应机制和制定免疫相关疾病的治疗策略至关重要。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CD40 抗体 (HM40-3) 参考文献:

  1. CD40信号传导的分子机制。  |  Bishop, GA. and Hostager, BS. 2001. Arch Immunol Ther Exp (Warsz). 49: 129-37. PMID: 11348017
  2. CD40在人类口腔上皮中的作用。  |  Villarroel Dorrego, M., et al. 2007. Oral Oncol. 43: 626-33. PMID: 17307023
  3. CD40-CD40配体在氧化应激、炎症和血管疾病中的相互作用。  |  Rizvi, M., et al. 2008. Trends Mol Med. 14: 530-8. PMID: 18977174
  4. CD40L及其受体CD40在动脉粥样硬化中的多功能性。  |  Lievens, D., et al. 2009. Thromb Haemost. 102: 206-14. PMID: 19652870
  5. 心血管疾病中的CD40-CD40L系统。  |  Pamukcu, B., et al. 2011. Ann Med. 43: 331-40. PMID: 21244217
  6. 移植模型中CD40和CD154阻断的最新进展。  |  Zhang, T., et al. 2015. Immunotherapy. 7: 899-911. PMID: 26268734
  7. CD40/CD40配体二聚体及其下游效应分子ISG54在急性神经炎症与持续性、进行性脱髓鞘之间的关系。  |  Hazra, B. and Das Sarma, J. 2024. IUBMB Life. 76: 313-331. PMID: 38116887
  8. 低分化肝细胞癌细胞通过CD40-CD40配体链路与T细胞相互作用，从而避免细胞凋亡。  |  Vinh Hanh, N., et al. 2024. Am J Pathol. 194: 1230-1247. PMID: 38548267
  9. 正常、反应性和恶性淋巴造血组织中的CD40/CD40配体相互作用。  |  Gruss, HJ., et al. 1997. Leuk Lymphoma. 24: 393-422. PMID: 9086433
  10. CD40-CD40配体在自身免疫性疾病中的相互作用。  |  Datta, SK. and Kalled, SL. 1997. Arthritis Rheum. 40: 1735-45. PMID: 9336404

订购信息

产品名称产品编号规格价格数量收藏夹

CD40 抗体 (HM40-3)

sc-20010
200 µg/ml
$316.00

CD40 抗体 (HM40-3) L

sc-20010 L
200 µg/0.1 ml
$316.00

This antibody is giving another band at around 54kD (other than the 43kD band). The results are consistent. Is its another isoform of CD40, that it is detecting? Its a virus-induced CD40 expression that we are checking.

Asked by: DEBANJANA
Thank you for your question. According to the literature, CD40 is a glycoprotein that could be detected at higher molecular weights.
Answered by: Technical Support
Date published: 2019-05-25

To do the Western blot of this antibody, how can I get the HRP conjugated anti hamster IgM? Would do please tell me the order information if possible?

Asked by: Ying2626
CD40 Antibody (HM40-3) is a Armenian hamster monoclonal IgM. Unfortunately, we do not currently offer an isotype-specific secondary antibody that is anti-Armenian hamster IgM-HRP. You could try using our mouse anti-Armenian hamster IgG-HRP: sc-2789, which may also be suitable for detection of IgM.
Answered by: Technical Support
Date published: 2019-04-20

what if the antibody is kept at room temperature for more than 2 days accidentally? will it still work?

Asked by: debby
Thank you for the question. We suggest storing this antibody at +4°C - 8°C in the fridge. If stored accidentally at room temp. for 2 days the product likely will not be harmed. We still suggest evaluating the antibody with a positive control and verify the obtained data.
Answered by: Technical Support Europe
Date published: 2018-01-31

How can CD40 (HM40-3): sc-20010 mouse monoclonal antibody be used for double or triple staining, if the other primary antibodies are also raised in mouse?

Asked by: jerojero
In this case, we recommend using a directly conjugated mouse monoclonal primary antibody. This antibody is available conjugated to phycoerythrin (sc-20010 PE) or fluoroscein (sc-20010 FITC).
Answered by: Technical Support
Date published: 2017-02-25
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Rated 5 out of 5 by from Great WB data obtained using mouse peripheralGreat WB data obtained using mouse peripheral blood lysate. -SCBT QC
Date published: 2015-06-28
Rated 4 out of 5 by from Good FCM data of WEHIGood FCM data of WEHI-231 cells. -SCBT QC
Date published: 2015-05-03
Rated 4 out of 5 by from Good immunoperoxidase membrane staining inGood immunoperoxidase membrane staining in formalin fixed, paraffin-embedded mouse lung tissue. -SCBT QC
Date published: 2015-03-17
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CD40 抗体 (HM40-3) is rated 4.3 out of 5 by 3.
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