CD3-zeta抗体 (6B10.2) 推荐用于 mouse, rat 和 human 来源的CD3-ζ WB, IP, IF, IHC(P) 和 FCM检测

CD3-ζ 抗体 (6B10.2): sc-1239

CD3-ζ抗体 (6B10.2) is rated 5.0 out of 5 by 21.
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说明书

    • 抗-CD3-zeta 抗体 6B10.2 是小鼠单克隆 IgG1 κ,CD3-ζ抗体, 在245篇文献中引用,规格为200 µg/ml
    • raised against amino acids 36-54 mapping within an N-terminal domain of CD3-ζ of human origin
    • 抗-CD3-zeta 抗体 (6B10.2) 推荐用于 WB, IP, IF, IHC(P) 和 FCM,检测mouse, rat 和human 来源的 CD3-ζ
    • 抗-CD3-zeta 抗体 (6B10.2) 可偶联 琼脂糖 用于 IP; HRP 用于 WB, IHC(P) 和 ELISA; 还可偶联 藻红蛋白 或者 FITC 用于 IF, IHC(P) 和 FCM
    • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
    • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
    • 还可偶联PerCP , PerCP-Cy5.5 以及 Alexa Fluor® 405 用于IF, IHC(P) and FCM
    • 关于如何获取CD3-ζ (6B10.2): sc-1239免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
    • m-IgG Fc BP-HRP (mouse IgG Fc binding protein-HRP) and m-IgGκ BP-HRP (mouse IgGκ binding protein-HRP) are the preferred secondary detection reagents for CD3-ζ Antibody (6B10.2) for WB and IHC(P) applications. These reagents are now offered in bundles with CD3-ζ Antibody (6B10.2) (see ordering information below).

In the description "raised against amino acids 36-54 mapping within an N-terminal domain of CD3-ζ of human origin" However, aa 36-54 is almost in the TM domain (31-51). How do you define aa 36-54? with or without leader? can it detect cytoplasmic one

Asked by: Leo_
Thank you for your question. The exact epitope recognized by this mouse monoclonal antibody has not yet been determined. We do have IHC(P) data on our website using this antibody to detect membrane and cytoplasmic staining in formalin-fixed paraffin-embedded tissue sections.
Answered by: Technical Support
Date published: 2020-10-23

Is the agarose-conjugated CD3-ζ Antibody (6B10.2): sc-1239 provided as 500µg/mL, unlike the other formats that come as 200µg/mL? The recommended concentration for IP is 1-2µg/mL--does this mean that you would only need 2-4µL agarose-conj. Ab per 1mL IP?

Asked by: eleonard
Thank you for your question. For the agarose conjugated antibody, sc-1239 AC, we recommend using ~ 20uL of a 25% slurry, which equals 10 ug of ab/5 uL of agarose beads. Each vial contains 500 ug of antibody/250 uL of agarose in 1 mL of slurry (PBS + Sodium Azide + conjugated antibodies).
Answered by: Technical Support
Date published: 2019-01-26

What is the recommended dilution for IHC/P application?

Asked by: UB948
Thank you for your question. The recommended starting dilution for IHC(P) as described in the antibody’s datasheet is 1:50 with a dilution range of 1:50-1:500.
Answered by: SCBT Heidelberg Support
Date published: 2019-01-06

I am using the LiCor/Odyssey system. Which secondary antibody do you recommend using with CD3-ζ Antibody (6B10.2): sc-1239?

Asked by: AbPolly
We recommend using one of our our exclusive Mouse IgG Binding Proteins (m-IgGκ) conjugated to CFL 680 or CFL 790. They are suitable for use with Near-Infrared (NIR) detection systems, such as LI-COR/Odyssey and other comparable systems. A complete list of available binding proteins is available on our website here: https://www.scbt.com/scbt/browse/support-products-mouse-igg-binding-proteins/_/N-ecrety
Answered by: Technical Support 12
Date published: 2017-01-10

I am going to be performing IHC in mouse tissues. How can I expect this antibody to work?

Asked by: Cweed
We recommend using a directly conjugated primary antibody. This product, sc-1239, is available conjugated to FITC, AF647, and AF488.
Answered by: TechService7
Date published: 2017-01-04
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Rated 5 out of 5 by from 特異的な反応が見られた ウエスタンブロットにおいて他社製品の抗CD3ζ抗体で検出出来なかったタンパク質が、この製品では検出することが出来ました。
Date published: 2020-05-29
Rated 5 out of 5 by from anti-CD3z Antibody The antibody usualy comes in very quickly; however, this order took a while because they were on backorder. Still, no problem. The antibody works great, with very clear blotting. Highly recommend!
Date published: 2018-08-29
Rated 5 out of 5 by from 16 KDa band This antibody is very good quality , 1:1000, still strong signal. I got only one specific band at 16 KDa
Date published: 2017-11-21
Rated 5 out of 5 by from Great product! I got this product two months ago. It did a great job when i used it to detect the CD3+ T cells by FCM. The high purity and specificity makes this antibody works well!
Date published: 2017-09-10
Rated 5 out of 5 by from we used it to detect the cd3ζ of CAR T cells I bought this a year ago and used it to detect the cd3ζ of CAR T cells by WB.As it turned out, this product is enable for this experiment.
Date published: 2017-09-04
Rated 5 out of 5 by from Specific band We used at 1:2000 for detection in Jurkat cells, and got only one specific band at 15kd
Date published: 2017-08-18
Rated 5 out of 5 by from good result used in WB I always had good result s with this CD3-ζ detection use this antibody.1:600 diluted the antibody.and use the goat anti-mouse secondary from Jackson.I suggest use ECL system from Santa cruz,it suiable for weak expression detection.
Date published: 2017-07-22
Rated 5 out of 5 by from strong and specific signal I used this antibody on breast cancer cell MCF7 and 231. The western band is clear and bright.
Date published: 2017-05-13
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