Date published: 2025-9-6

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CARP 抗体 (G-2): sc-365056

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说明书
  • CARP 抗体 G-2 是小鼠单克隆 IgG1 κ,CARP抗体, 在16篇文献中引用,规格为200 µg/ml
  • 免疫原氨基酸序列1-120位于human物种的CARP的N-terminus
  • CARP 抗体 (G-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 CARP
  • 抗CARP抗体(G-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取CARP (G-2): sc-365056免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 1 BP-HRP">m-IgG1 BP-HRP是CARP Antibody (G-2) 适用于 WB 应用。 的首选辅助检测试剂。该试剂现与CARP Antibody (G-2) 打包提供(请参阅下面的订购信息)。

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関連項目

CARP 抗体 (G-2) 是一种小鼠单克隆 IgG1 kappa 轻链抗体,可通过免疫印迹 (WB)、免疫沉淀 (IP)、免疫荧光 (IF) 和酶联免疫吸附 (ELISA) 检测小鼠、大鼠和人 CARP 蛋白。CARP (G-2) 抗体有非共轭型和多种共轭型,包括琼脂糖、辣根过氧化物酶 (HRP)、藻红蛋白 (PE)、异硫氰酸荧光素 (FITC) 和多种 Alexa Fluor® 共轭物。心脏强蛋白重复蛋白(CARP)在调节心脏基因表达方面起着至关重要的作用,尤其是在应对心脏肥大等应激条件时。CARP 主要位于细胞核中,对于调节心脏功能相关基因的转录活性至关重要。在腹主动脉收缩的大鼠和终末期心力衰竭患者等病理情况下,CARP 的表达会明显上调,这凸显了 CARP 作为心脏肥大遗传标记的潜力。值得注意的是,CARP 与心脏同源基因 Nkx2.5 相互作用,抑制其对心房利钠因子启动子的转录激活,而心房利钠因子对维持正常心脏功能至关重要。此外,CARP 的表达还受到钙离子结合蛋白的影响,钙离子结合蛋白是心脏肌质网中的一种主要钙结合蛋白,这可能进一步将 CARP 与心脏肥大和纤维化的发展联系起来。此外,研究还发现转化生长因子β(TGFβ)可诱导血管平滑肌细胞中 CARP 的表达,这表明 CARP 可能在介导 TGFβ 对细胞增殖的抑制作用方面发挥作用,从而促进心血管健康。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

CARP 抗体 (G-2) 参考文献:

  1. 心脏杏仁蛋白重复蛋白是心肌肥大的新型标记物:启动子中 M-CAT 元件的作用  |  Aihara, Y., et al. 2000. Hypertension. 36: 48-53. PMID: 10904011
  2. 转化生长因子-β/Smads 信号通过 CAGA 矩阵在血管平滑肌细胞中诱导细胞类型受限的 ankyrin 重复蛋白 CARP 基因转录。  |  Kanai, H., et al. 2001. Circ Res. 88: 30-6. PMID: 11139470
  3. Myopalladin是一种新型的145千道尔顿肉瘤蛋白,在Z-盘和I-带蛋白组装中具有多重作用。  |  Bang, ML., et al. 2001. J Cell Biol. 153: 413-27. PMID: 11309420
  4. 心肌杏仁蛋白重复蛋白是心脏基因表达的负调控因子,在人类心力衰竭中会增加。  |  Zolk, O., et al. 2002. Biochem Biophys Res Commun. 293: 1377-82. PMID: 12054667
  5. 转基因小鼠心脏过表达钙离子拮抗剂的基因表达调控。  |  Ihara, Y., et al. 2002. Cell Calcium. 32: 21-9. PMID: 12127059
  6. CARP 是一种心肌钝蛋白重复蛋白,在伤口愈合过程中会上调,并诱导实验性肉芽组织中的血管生成。  |  Shi, Y., et al. 2005. Am J Pathol. 166: 303-12. PMID: 15632022
  7. 多功能蛋白质:心脏杏仁蛋白重复蛋白。  |  Zhang, N., et al. 2016. J Zhejiang Univ Sci B. 17: 333-41. PMID: 27143260
  8. 心脏强蛋白重复蛋白通过上调 Bcl-2 的表达减轻心肌细胞凋亡。  |  Zhang, N., et al. 2016. Biochim Biophys Acta. 1863: 3040-3049. PMID: 27713078
  9. 心力衰竭中心脏杏仁蛋白重复蛋白的调节改变  |  Kempton, A., et al. 2018. Heliyon. 4: e00514. PMID: 29560432
  10. 心脏锚蛋白重复蛋白导致扩张型心肌病和心力衰竭。  |  Zhang, N., et al. 2021. FASEB J. 35: e21488. PMID: 33734499
  11. CARP是一种心脏锚蛋白重复蛋白,是Nkx2-5同源染色体基因通路的下游。  |  Zou, Y., et al. 1997. Development. 124: 793-804. PMID: 9043061
  12. 多柔比星的新型心脏限制靶点。CARP:心脏祖细胞和心肌细胞基因表达的核调节器。  |  Jeyaseelan, R., et al. 1997. J Biol Chem. 272: 22800-8. PMID: 9278441

订购信息

产品名称产品编号规格价格数量收藏夹

CARP 抗体 (G-2)

sc-365056
200 µg/ml
$316.00

CARP (G-2): m-IgG1 BP-HRP 套装

sc-531698
200 µg Ab; 20 µg BP
$354.00

CARP 抗体 (G-2) AC

sc-365056 AC
500 µg/ml, 25% agarose
$416.00

CARP 抗体 (G-2) HRP

sc-365056 HRP
200 µg/ml
$316.00

CARP 抗体 (G-2) FITC

sc-365056 FITC
200 µg/ml
$330.00

CARP 抗体 (G-2) PE

sc-365056 PE
200 µg/ml
$343.00

CARP 抗体 (G-2) Alexa Fluor® 488

sc-365056 AF488
200 µg/ml
$357.00

CARP 抗体 (G-2) Alexa Fluor® 546

sc-365056 AF546
200 µg/ml
$357.00

CARP 抗体 (G-2) Alexa Fluor® 594

sc-365056 AF594
200 µg/ml
$357.00

CARP 抗体 (G-2) Alexa Fluor® 647

sc-365056 AF647
200 µg/ml
$357.00

CARP 抗体 (G-2) Alexa Fluor® 680

sc-365056 AF680
200 µg/ml
$357.00

CARP 抗体 (G-2) Alexa Fluor® 790

sc-365056 AF790
200 µg/ml
$357.00

Dear support team, would it be possible to receive a free sample of CARP Antibody (G-2): sc-365056 to test it before ordering larger amounts of antibody? Best wishes, Blaž

Asked by: Blaž
Thank you for your question. Please contact Technical Service or your local distributor for information on how to receive a free 10 ug sample of this monoclonal antibody.
Answered by: Tech Service
Date published: 2020-02-04

What is the recommended fixation method for immunofluorescence staining with CARP (G-2): sc-365056 monoclonal antibody?

Asked by: jenniferc
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2018-08-27
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Rated 4 out of 5 by from Good antibodyThe antibody works fine. It gives a strong signal at 43kDa in the WBs with the lysates processed from rat myocytes.
Date published: 2018-06-19
Rated 1 out of 5 by from Didn't work at all!I have got this antibody and I have used it for western blot on primary human fibroblast with several serial dilution (from 1:1000 to 1:200). I couldn't even see a single tiny band. Very bad!
Date published: 2018-01-26
Rated 4 out of 5 by from cardiac ankyrin repeat protein (CARP)good results in immunocytochemistry, shows sarcomere striation and nuclear labeling in ipsc-derived human cardiomyocytes
Date published: 2017-07-25
Rated 5 out of 5 by from Excellent!This antibody works great in western blot analysis of CARP expression in human heart tissue extract and Sol8 and NIH/3T3 nuclear extracts.
Date published: 2017-01-18
Rated 4 out of 5 by from Good bands seen in WB using human heart tissueGood bands seen in WB using human heart tissue extract and Sol8 and NIH/3T3 nuclear extracts. -SCBT QC
Date published: 2015-05-08
Rated 4 out of 5 by from Band of interest seen in WB using VEGF treated HUVBand of interest seen in WB using VEGF treated HUV-EC-C whole cell lysate. -SCBT QC
Date published: 2015-04-20
Rated 5 out of 5 by from Exceptional immunofluorescence cytoplasmicExceptional immunofluorescence cytoplasmic staining in methanol-fixed HeLa cells. -SCBT QC
Date published: 2015-03-04
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CARP 抗体 (G-2) is rated 3.9 out of 5 by 7.
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