Date published: 2025-9-7

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αA-crystallin 抗体 (B-2): sc-28306

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说明书
  • alpha A-crystallin 抗体 B-2 是小鼠单克隆 IgG2a κ,αA-crystallin抗体, 在19篇文献中引用,规格为200 µg/ml
  • 免疫1-173氨基酸,对应于human来源αA-crystallin全长
  • alpha A-crystallin 抗体 (B-2) 推荐用于 WB, IP, IF 和 ELISA,检测mouse, rat 和human 来源的 αA-crystallin; 可能与 αB-crystallin产生交联反应
  • 抗alpha A-crystallin抗体(B-2)可与琼脂糖结合用于IP;与HRP结合用于WB、IHC(P)和ELISA;与藻红蛋白FITC结合用于IF、IHC(P)和FCM
  • 还可偶联Alexa Fluor® 488, Alexa Fluor® 546, Alexa Fluor® 594Alexa Fluor® 647,用于WB (RGB), IF, IHC(P) 和 FCM, 以及用于RGB荧光成像系统,例如iBright™ FL1000, FluorChem™, Typhoon, Azure和其他类似的系统
  • 还可偶联Alexa Fluor® 680Alexa Fluor® 790, 用于WB (NIR), IF 和 FCM; 以及用于近红外(NIR)检测系统,如LI-COR®/Odyssey®, iBright™ FL1000, FluorChem™, Typhoon, Azure和类似系统
  • 关于如何获取αA-crystallin (B-2): sc-28306免费10 µg小样,联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • m-IgG Fc BP-HRP2a BP-HRP">m-IgG2a BP-HRPm-IgGκ BP-HRP是αA-crystallin Antibody (B-2) 适用于 WB 应用。 的首选辅助检测试剂。这些试剂现与αA-crystallin Antibody (B-2) 打包提供(请参阅下面的订购信息)。

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関連項目

αA-crystallin Antibody (B-2)是一种小鼠单克隆IgG2a kappa轻链抗体,可通过蛋白质印迹(WB)、免疫沉淀(IP)、免疫荧光(IF)和酶联免疫吸附测定(ELISA )。αA-crystallin 单克隆抗体(B-2)有非结合型和多种结合型,包括琼脂糖、辣根过氧化物酶(HRP)、藻红蛋白(PE)、异硫氰酸荧光素(FITC)和多种 Alexa Fluor® 结合物。αA-crystallin在维持脊椎动物眼晶状体的透明度和折射率方面起着至关重要的作用,而眼晶状体的透明度和折射率对于正常视力至关重要。作为小热休克蛋白家族的一员,αA-crystallin具有分子伴侣的功能,有助于稳定变性蛋白并防止聚集,但αA-crystallin并不促进其复性。αA-crystallin主要在晶状体中表达,αA-crystallin基因突变可导致常染色体显性遗传性先天性白内障,这凸显了αA-crystallin在晶状体完整性和功能方面的重要性。此外,αA-crystallin与其他晶状体蛋白和参与细胞应激反应的蛋白相互作用,进一步强调了αA-crystallin在维持细胞稳态和抵御蛋白毒性方面的意义。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

αA-crystallin 抗体 (B-2) 参考文献:

  1. αB-结晶素中的 R120G 突变与 desmin 相关性肌病有关,会导致结构不规则和伴侣样功能缺陷。  |  Bova, MP., et al. 1999. Proc Natl Acad Sci U S A. 96: 6137-42. PMID: 10339554
  2. α-结晶素可防止蛋白质发生不可逆变性,并与其他热休克蛋白协同作用,以依赖 ATP 的方式使稳定的部分变性蛋白质重新变性。  |  Wang, K. and Spector, A. 2000. Eur J Biochem. 267: 4705-12. PMID: 10903503
  3. 晶状体及其微生物同源物:结构, 稳定性和功能。  |  Jaenicke, R. and Slingsby, C. 2001. Crit Rev Biochem Mol Biol. 36: 435-99. PMID: 11724156
  4. α-结晶素型热休克蛋白:多伴侣网络中的社会化小伴侣。  |  Narberhaus, F. 2002. Microbiol Mol Biol Rev. 66: 64-93; table of contents. PMID: 11875128
  5. 小型热休克蛋白α-B-结晶素是一种新型的TRAIL诱导细胞凋亡抑制剂,可抑制caspase-3的活化。  |  Kamradt, MC., et al. 2005. J Biol Chem. 280: 11059-66. PMID: 15653686
  6. αA-结晶素在实验性自身免疫性葡萄膜炎中的作用。  |  Wang, L., et al. 2015. Curr Mol Med. 15: 558-64. PMID: 26238368
  7. αA-结晶素的膜插入取决于寡聚体的大小。  |  Tjondro, HC., et al. 2016. Biochem Biophys Res Commun. 473: 1-7. PMID: 26975472
  8. 外源性αA-结晶素在视网膜神经节细胞中发挥旁分泌保护作用的证据  |  Nath, M., et al. 2022. eNeuro. 9: PMID: 35168949
  9. αA-结晶素介导的视网膜神经元神经保护作用与蛋白激酶 B 无关  |  Nath, M. and Fort, PE. 2022. Front Neurosci. 16: 912757. PMID: 35669493
  10. 骨骼肌在持续收缩活动中 B-crystallin 和 Hsp27 的差异表达。与肌源性调节因子的关系  |  Neufer, PD. and Benjamin, IJ. 1996. J Biol Chem. 271: 24089-95. PMID: 8798647
  11. 与人类阿尔法晶体蛋白基因 CRYAA 的错义突变有关的常染色体显性先天性白内障。  |  Litt, M., et al. 1998. Hum Mol Genet. 7: 471-4. PMID: 9467006
  12. 小型热休克蛋白αB-结晶素具有可变的四元结构。  |  Haley, DA., et al. 1998. J Mol Biol. 277: 27-35. PMID: 9514758

订购信息

产品名称产品编号规格价格数量收藏夹

αA-crystallin 抗体 (B-2)

sc-28306
200 µg/ml
$316.00

αA-crystallin (B-2): m-IgG Fc BP-HRP 套装

sc-528415
200 µg Ab; 10 µg BP
$354.00

αA-crystallin (B-2): m-IgGκ BP-HRP 套装

sc-520789
200 µg Ab, 40 µg BP
$354.00

αA-crystallin (B-2): m-IgG2a BP-HRP 套装

sc-547090
200 µg Ab; 10 µg BP
$354.00

αA-crystallin 抗体 (B-2) AC

sc-28306 AC
500 µg/ml, 25% agarose
$416.00

αA-crystallin 抗体 (B-2) HRP

sc-28306 HRP
200 µg/ml
$316.00

αA-crystallin 抗体 (B-2) FITC

sc-28306 FITC
200 µg/ml
$330.00

αA-crystallin 抗体 (B-2) PE

sc-28306 PE
200 µg/ml
$343.00

αA-crystallin 抗体 (B-2) Alexa Fluor® 488

sc-28306 AF488
200 µg/ml
$357.00

αA-crystallin 抗体 (B-2) Alexa Fluor® 546

sc-28306 AF546
200 µg/ml
$357.00

αA-crystallin 抗体 (B-2) Alexa Fluor® 594

sc-28306 AF594
200 µg/ml
$357.00

αA-crystallin 抗体 (B-2) Alexa Fluor® 647

sc-28306 AF647
200 µg/ml
$357.00

αA-crystallin 抗体 (B-2) Alexa Fluor® 680

sc-28306 AF680
200 µg/ml
$357.00

αA-crystallin 抗体 (B-2) Alexa Fluor® 790

sc-28306 AF790
200 µg/ml
$357.00

What reagents are used to dilute the αA-crystallin(B-2) antibodies?

Asked by: Anonymous
Thank you for your question. For information specific to your application, please refer to the link below and select the appropriate protocol. https://www.scbt.com/resources/protocols
Answered by: Santa Cruz TS
Date published: 2021-07-10

What is the recommended fixation method for immunofluorescence staining with αA-crystallin (B-2): sc-28306 monoclonal antibody?

Asked by: Randy McDonald
Thank you for your inquiry. We recommend fixing cells for 5 minutes in -10°C methanol and allowing to air dry. The full protocol can be viewed here: https://www.scbt.com/scbt/resources/protocols/immunofluorescence-cell-staining
Answered by: Technical Support
Date published: 2017-02-24
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Rated 1 out of 5 by from Non funzionaleAbbiamo testato questo anticorpo sia per biochimica che per immunofluorescenza, impiegando come controllo la proteina over-espressa. Il prodotto sc-28306 non sembra essere specifico.
Date published: 2020-07-09
Rated 5 out of 5 by from Strong and reliable for IHC and WBVery strong and specific, this alpha A-crystallin is a great choice for WB and IF applications. Clear staining in IF and nice solid bands are reliably seen in WB.
Date published: 2017-01-13
Rated 5 out of 5 by from Used it in WB at 1:500 dilution and worked fineUsed it in WB at 1:500 dilution and worked fine.
Date published: 2015-07-07
Rated 5 out of 5 by from Large amount of signal observed in WB inLarge amount of signal observed in WB in mouse eye and rat eye tissue extracts. -SCBT QC
Date published: 2015-05-25
Rated 5 out of 5 by from Published data for WB in mouse corneasPublished data for WB in mouse corneas. -SCBT Publication Review
Date published: 2015-03-14
Rated 5 out of 5 by from Positive result in WB in human eye tissue extractPositive result in WB in human eye tissue extract. -SCBT QC
Date published: 2015-02-13
Rated 5 out of 5 by from Band of interest observed in WB in both nonBand of interest observed in WB in both non-transfected and human B-crystallin transfected 293 whole cell lysates. -SCBT QC
Date published: 2015-01-31
Rated 5 out of 5 by from Published data for WB in rat neural retinasPublished data for WB in rat neural retinas. -SCBT Publication Review
Date published: 2015-01-23
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αA-crystallin 抗体 (B-2) is rated 4.6 out of 5 by 10.
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