Date published: 2025-9-11
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8-OHdG 抗体 (15A3): sc-66036

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说明书
  • 8-OHdG 抗体 15A3 是小鼠单克隆 IgG2b, 在222篇文献中引用，提供 100 µg/ml
  • 针对8-羟基鸟苷（8-OHdG）-BSA和-酪蛋白结合物
  • 推荐用于 8-OHdG (8-hydroxy-2'-deoxyguanosine, 8-hydroxyguanine and 8-hydroxyguanosine) IF, IHC(P) 和 ELISA检测
  • 看 8-OHdG (E-8): sc-393871，可查看有标记的 8-OHdG 抗体，包括AC, HRP, FITC, PE, Alexa Fluor® 488, 594, 647, 680 和 790
  • 关于如何获取8-OHdG (15A3): sc-66036的免费10 µg小样，联系我们技术服务部门 (或者您当地的代理商)了解详情。
  • 目前，我们还没有完成8-OHdG 抗体 (15A3)的首选二抗检测试剂的鉴定。这项工作正在进行中。

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研究信息

関連項目

8-OHdG抗体（15A3）是一种IgG2b型小鼠单克隆8-OHdG抗体，可通过免疫荧光（IF）、免疫组化（IHC-P）和酶联免疫吸附试验（ELISA）检测8-OHdG（8-羟基-2'-脱氧鸟苷、8-羟基鸟嘌呤和8-羟基鸟苷酸）。8-OHdG抗体（15A3）以非偶联的抗8-OHdG抗体形式提供。DNA或RNA损伤会阻碍细胞执行其功能，并可能显著增加肿瘤形成的可能性。DNA和RNA损伤的原因之一是碱基的氧化。8-羟基-2'-脱氧鸟苷、8-羟基鸟嘌呤（8-OHdG）和8-羟基鸟苷酸都是RNA和DNA氧化损伤的标志物。8-羟基-2'-脱氧鸟苷是由活性氧和氮物种产生的，包括羟基自由基和过氧亚硝酸盐。8-羟基鸟嘌呤是参与突变的主要碱基损伤之一，由电离辐射和放射模拟剂引起。8-羟基鸟苷酸在DNA中诱导G到T的颠换，这可能是致突变的。当研究这种类型损伤的影响时，DNA和RNA损伤的标志物是有用的研究工具。

仅限研究使用。不适用于诊断和治疗用途。

Alexa Fluor® 是Molecular Probes Inc., OR., USA的商标

LI-COR®和 Odyssey® 是LI-COR Biosciences的注册商标

8-OHdG 抗体 (15A3) 参考文献:

  1. 帕金森病患者脑脊液和血清中 8-羟基鸟苷浓度的变化。  |  Abe, T., et al. 2003. Neurosci Lett. 336: 105-8. PMID: 12499051
  2. 某些微卫星稳定的结直肠癌细胞存在 8-羟基鸟苷修复缺陷。  |  Parker, AR., et al. 2002. Cancer Res. 62: 7230-3. PMID: 12499263
  3. 在缺乏 8-羟基鸟嘌呤-DNA 糖基化酶的情况下，Ig 基因发生正常的体细胞超突变。  |  Winter, DB., et al. 2003. J Immunol. 170: 5558-62. PMID: 12759433
  4. 在 Myh 和 Ogg1 DNA 糖基化酶都有缺陷的肿瘤易感小鼠 DNA 中，氧化碱基病变 8-羟基鸟嘌呤的积累。  |  Russo, MT., et al. 2004. Cancer Res. 64: 4411-4. PMID: 15231648
  5. 特发性扩张型心肌病患者体内 8- 羟基-2'-脱氧鸟苷水平的意义。  |  Watanabe, E., et al. 2006. J Card Fail. 12: 527-32. PMID: 16952786
  6. 紫外线诱导的寡核苷酸中 8-羟基鸟嘌呤降解及其对酵母寡核苷酸转化试验活性的影响  |  Okugawa, Y., et al. 2004. Nucleic Acids Symp Ser (Oxf). 287-8. PMID: 17150591
  7. 金属离子结合在从核苷 2'-deoxyguanosine 和核苷酸 2'-deoxyguanosine-5'-monophosphate 生成 8-hydroxy-2'-deoxyguanosine 中的作用。  |  Noblitt, SD., et al. 2007. J Inorg Biochem. 101: 536-42. PMID: 17234269
  8. 乳腺癌患者尿液中的 8-hydroxy-2'-deoxyguanosine (8-OHdG) 和基因多态性。  |  Kuo, HW., et al. 2007. Mutat Res. 631: 62-8. PMID: 17512776
  9. 8-氧代-7,8-二氢-2'-脱氧鸟苷（8-oxodG）和8-羟基-2'-脱氧鸟苷（8-OHdG）作为妊娠糖尿病（GDM）发展的潜在生物标志物。  |  Urbaniak, SK., et al. 2020. Molecules. 25: PMID: 31947819
  10. CypD诱导的ROS输出通过8-OHdG/NLRP3/MMP9途径促进颅内动脉瘤的形成和破裂。  |  Fan, H., et al. 2023. Redox Biol. 67: 102887. PMID: 37717465
  11. 使用8-羟基-2'-脱氧鸟苷（8-OHdG）作为评估与糖尿病相关的牙周疾病的可靠生物标志物。  |  Goriuc, A., et al. 2024. Int J Mol Sci. 25: PMID: 38338704
  12. 8- 羟基鸟苷在人类乳腺癌发生过程中的预后和致病相关性。  |  Musarrat, J., et al. 1996. Eur J Cancer. 32A: 1209-14. PMID: 8758255

订购信息

产品名称产品编号规格价格数量收藏夹

8-OHdG 抗体 (15A3)

sc-66036
100 µg/ml
$316.00

I have tried 1:50 ,1:100 and 1:200 dilutions for immunofluorescence in human endometrial tissue samples. only cytoplasmic and perinuclear staining was seen. nuclear staining was not observed. please advice.

Asked by: swati
Thank you for your question. For troubleshooting advice, please contact our technical service team directly.
Answered by: Tech Support Europe
Date published: 2025-01-16

Can u tell me what dilution range should i use to obtain nuclei staining in breast carcinoma cell? Bcs I've already tried 1:50,100,200 still stains the cytoplasm, not nuclei. Myb there is a step-by-step guide that I can read. Thanks

Asked by: fsplays16
Thank you for your question. We recommend a starting dilution of 1:50-1:500. https://www.scbt.com/resources/protocols/immunofluorescence-cell-staining
Answered by: BlakeJ
Date published: 2024-03-28

Please advice me. I use 8-OHdG Antibody (15A3) sc-66036 to perform IHC on breast carcinoma cell (PE). Can you tell me where 8-OHdG should stain the cell, is it in nuclei or cytoplasm? Because I've tried many dilution range but still in cytoplasm.

Asked by: fsplays16
Thank you for your question. 8-OHdG is found in the nuclei .
Answered by: BlakeJ
Date published: 2025-01-03

Does sc-66036 detect 8-oxo G in RNA? I wish to use it form doing IF in S. cerevisiae cells? Can you recommend protocol for same?

Asked by: Purusharth I Rajyaguru
Thank you for your question. You can find our recommended protocols under this link: https://www.scbt.com/resources/protocols
Answered by: Tech Support Europe
Date published: 2022-08-03

Hello. I have mouse brain sections made in vibratome and my question is, Which is the ideal antibody to detect 8-OHdG in this kind of samples? Can i use this one?

Asked by: Marc111
Thank you for your question. This antibody is not species specific and should work in mouse samples.
Answered by: Tech Support Europe
Date published: 2022-05-10

Hello, I am using sc-66036 for western blot: what is the expected molecular weight of the band? And what does it tell me (is it genomic DNA? oxidized proteins?)? Thank you.

Asked by: Anonymous
Thank you for your question. 8-oxo-dG is a marker for oxidative stress in a cell. This antibody is not recommended for western blotting, as there would be no distinct protein this antibody detects. It is designed for IF, IHC(P) and ELISA applications.
Answered by: Tech Support Europe
Date published: 2021-11-22

Hi, in how many patients tissues  can we use one 8-OHdG Antibody (15A3): sc-66036 for the estimation of number of sample size in study?

Asked by: heraa
Thank you for your question. The antibody is provided as 200µg in 1ml. We recommend a starting dilution of 1:100 for Western blot, and 1:50 for IHC applications, so it's very hard to estimate how many experiments you can perform with one vial.
Answered by: Tech Service
Date published: 2021-11-22

Can I use 8-OHdG Antibody (15A3)sc-66036 to perform immunohistochemistry/immunofluroscence on zebrafish brain and also suggest the secoundry antibody for the same.

Asked by: abhishekjain
Thank you for your question. Yes, this antibody is suitable for IF/IHC(P), and there is no specific species reactivity since this antibody detects 8-OHdG, a marker of oxidative damage to RNA and DNA. We are aware of one product citation using this antibody in zebrafish samples. Please refer to PMID: 28550782 for the details. We would recommend using one of our fluorescently conjugated mouse IgG kappa binding proteins as a secondary antibody for detection. A complete list of avaialble conjugates is available below. https://www.scbt.com/scbt/browse/Secondary-Antibodies/_/N-1hoaxk4
Answered by: Technical Support
Date published: 2019-07-13
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Rated 4 out of 5 by from GreatThe wonderful IHC data were produced using this antibody.
Date published: 2024-10-16
Rated 4 out of 5 by from Good resultsDilution 1:250 IHC rat kidney tissues. Very strong and staining.
Date published: 2021-06-16
Rated 4 out of 5 by from 抗体機能が良いmtDNA damageの研究に使える抗体を探していました。 此の度サンプルとして頂きました。 早速試したところWBもIPも可能なことが判明しました。
Date published: 2021-02-03
Rated 3 out of 5 by from RPE immunofluorescence stainingClear perinuclear immunofluorescence taining in formalin-fixed, paraffin-embedded retinal pigment epithelium of retinal rat new born. 1/50 dilution
Date published: 2020-05-07
Rated 5 out of 5 by from 免疫组化DAB染色很不错先使用了sample，效果很好。又购买了一支，染色结果很漂亮。 fffff
Date published: 2019-12-24
Rated 4 out of 5 by from a nice marker for DNA oxidationI did IHC (P) study with colon tissue, the results are pretty good.
Date published: 2018-11-08
Rated 5 out of 5 by from for IHCWorks well for IHC with human paraffin-embedded kidney tissue
Date published: 2017-05-13
Rated 4 out of 5 by from Good stainingMultiple publications cite this antibody for quality staining in IHC, including paraffin-embedded sections.
Date published: 2017-01-10
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8-OHdG 抗体 (15A3) is rated 4.1 out of 5 by 14.
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