IL-10 Antibody (2G101H7): sc-57245

IL-10 Antibody (2G101H7) is a mouse monoclonal IgG1 antibody that detects interleukin-10 in mouse and rat samples through applications such as western blotting (WB), flow cytometry (FCM), and enzyme-linked immunosorbent assay (ELISA). IL-10 is a crucial cytokine primarily secreted by TH2 clones, playing a significant role in immune regulation by suppressing cytokine production from TH1 clones in response to antigens presented by monocyte and macrophage antigen presenting cells. This suppression is vital for maintaining immune homeostasis and preventing excessive inflammatory responses, which can lead to tissue damage. Additionally, IL-10 inhibits the activation of cytokine-targeted cells, potentially through the downregulation of CD25, the IL-2 receptor, on macrophages and B lymphocytes. Notably, human and murine IL-10 share a high degree of sequence identity, with 81% similarity at the amino acid level, indicating conserved functions across species. The biological activity of IL-10 is mediated through IL-10R, a glycoprotein that can be induced in cultured macrophages and fibroblasts by lipopolysaccharide stimulation. Elevated levels of IL-10 have been observed in individuals infected with HIV-1, suggesting involvement in disease progression, making anti-IL-10 antibody (2G101H7) an essential tool for researchers studying immune responses and related pathologies.

The preferred IL-10 antibody is IL-10 Antibody (E-10): sc-8438.